Effects of mutations on unliganded gating
| Construct | f0 | ±SE | b0 | ±SE | E0 | ±SE | Fold change E0 | Fold change E2choline | n |
| βδ9′S | 351 | 34 | 5,021 | 534 | 0.074 | 0.01 | – | – | 9 |
| +αN217K | 1,262 | 62 | 223 | 24 | 5.9 | 0.90 | 81 | 43 | 4 |
| +αL279W | 1,855 | 102 | 147 | 21 | 13.4 | 2.66 | 182 | 156 | 3 |
| +αC418W | 2,402 | 205 | 286 | 43 | 8.7 | 0.76 | 117 | 110 | 4 |
| +α(N217T,C418Y) | 1,942 | 128 | 79 | 8 | 24.9 | 1.15 | 338 | 440 | 3 |
| +αS269I | 1,708 | 94 | 266 | 6 | 6.4 | 0.22 | 87 | 115 | 2 |
| +αP272A | 1,656 | 336 | 209 | 28 | 7.9 | 0.83 | 107 | 237 | 3 |
| DYS | 187 | 48 | 3,994 | 11,106 | 0.047 | 0.008 | – | – | 8 |
| εP121La | 229 | 17 | 5,068 | 285 | 0.046 | 0.01 | 1 | – | 5 |
| DYS+βL269D | 532 | 39 | 2,739 | 232 | 0.19 | 0.003 | – | – | 3 |
| εP121Rb | 23 | 4 | 5,633 | 255 | 0.0042 | 0.001 | 0.02 | – | 3 |
| Construct | f0 | ±SE | b0 | ±SE | E0 | ±SE | Fold change E0 | Fold change E2choline | n |
| βδ9′S | 351 | 34 | 5,021 | 534 | 0.074 | 0.01 | – | – | 9 |
| +αN217K | 1,262 | 62 | 223 | 24 | 5.9 | 0.90 | 81 | 43 | 4 |
| +αL279W | 1,855 | 102 | 147 | 21 | 13.4 | 2.66 | 182 | 156 | 3 |
| +αC418W | 2,402 | 205 | 286 | 43 | 8.7 | 0.76 | 117 | 110 | 4 |
| +α(N217T,C418Y) | 1,942 | 128 | 79 | 8 | 24.9 | 1.15 | 338 | 440 | 3 |
| +αS269I | 1,708 | 94 | 266 | 6 | 6.4 | 0.22 | 87 | 115 | 2 |
| +αP272A | 1,656 | 336 | 209 | 28 | 7.9 | 0.83 | 107 | 237 | 3 |
| DYS | 187 | 48 | 3,994 | 11,106 | 0.047 | 0.008 | – | – | 8 |
| εP121La | 229 | 17 | 5,068 | 285 | 0.046 | 0.01 | 1 | – | 5 |
| DYS+βL269D | 532 | 39 | 2,739 | 232 | 0.19 | 0.003 | – | – | 3 |
| εP121Rb | 23 | 4 | 5,633 | 255 | 0.0042 | 0.001 | 0.02 | – | 3 |
Backgrounds: βδ9′S, βL262S + δL265S; fold change E0 is relative to the background, and fold change E2choline is relative to the WT. The additional mutations have similar effects on the unliganded versus diliganded gating equilibrium constant. Fold change E2choline values are from Cadugan and Auerbach (2007; αL279W), Mitra et al. (2004; αC418W/Y [αC418Y alone increases E2choline by 71.3-fold]), Mitra et al. (2005; αS269I), and Jha et al. (2007; αP272A). Values for αN217K and αN217T were calculated from new measurements: f2choline = 2,543 ± 132; n > 3 patches; b2choline = 1,331 ± 109; E2: 1.96; and f2choline = 375 ± 44; b2choline = 1,319 ± 162; E2choline: 0.29. For α (N217T + C418Y) the fold change in E2choline was the product for the value of the individual mutants (6.15 × 71.3 = 440).
DYS, α(D97A + Y127F + S269I) (Purohit and Auerbach, 2009).
DYS + βL269D.