Table 1. Unliganded gating constants for... | Rockefeller University Press

Table 1.

Unliganded gating constants for loop-C glycine constructs

Construct	f₀	±SE	b₀	±SE	E₀	±SE	E₀^mut/wt	n
WT loop C	234	7	1,931	189	0.123	0.01	—	3
11gly	438	24	2,536	151	0.173	0.01	1.4	3
9gly	464	24	2,578	488	0.194	0.04	1.6	3
7gly	547	62	2,314	612	0.281	0.09	2.3	3
5gly	423	94	3,196	813	0.204	0.08	1.7	5
3gly	464	58	1,877	18	0.246	0.03	2.0	3

Construct	f₀	±SE	b₀	±SE	E₀	±SE	E₀^mut/wt	n
WT loop C	234	7	1,931	189	0.123	0.01	—	3
11gly	438	24	2,536	151	0.173	0.01	1.4	3
9gly	464	24	2,578	488	0.194	0.04	1.6	3
7gly	547	62	2,314	612	0.281	0.09	2.3	3
5gly	423	94	3,196	813	0.204	0.08	1.7	5
3gly	464	58	1,877	18	0.246	0.03	2.0	3

f₀ and b₀, unliganded opening and closing rate constants (s⁻¹); E₀, unliganded gating equilibrium constant (=f₀/b₀). Background: αA96H + εS450W (net fold increase in E₀ = 1.7 × 10⁵); n, number of patches.

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