Table 1.
Parameters of Boltzmann fits to GV, FV, SSI, and SSI-FV curves
Channel variantGV/FVSSI/SSI-FV
V1/2kPVPkV1/2kPVPk
 mV mV    mV mV   
GV and SSI          
WT −35.3 ± 2.5 8.5 ± 0.5    −83.6 ± 1.6 7.3 ± 0.4   
WT-LFS −36.0 ± 2.1 8.6 ± 0.4    −88.5 ± 1.3 6.4 ± 0.2   
DI-LFS −57.7 ± 1.7 7.5 ± 1.4    −78.0 ± 1.4 6.5 ± 0.2   
DII-LFS −36.4 ± 1.7 8.7 ± 1.1    −82.0 ± 1.5 7.7 ± 1.0   
DIII-LFS −39.9 ± 2.2 9.5 ± 0.4    −76.2 ± 1.2 6.7 ± 0.2   
DIV-LFS −33.4 ± 2.5 9.3 ± 0.4    −71.4 ± 1.0 10.0 ± 0.6   
IQM-LFS −30.9 ± 2.0 9.2 ± 0.2 0.1428 0.2269  −52.0 ± 2.1 7.3 ± 1.9 3.8 × 10−6 0.1770 
A1330P −36.0 ± 1.9 7.7 ± 0.4 0.8062 0.2825  −74.6 ± 1.1 6.6 ± 0.5 0.0017 0.2597 
N1325S −42.5 ± 0.3 2.6 ± 0.7 0.0689 3.2 × 10−4  −68.3 ± 3.7 5.4 ± 0.2 0.0045 0.0102 
N1659A −23.7 ± 2.4 9.7 ± 0.3 0.0085 0.1639  −52.6 ± 1.5 7.5 ± 1.0 1.6 × 10−7 0.7790 
DI-IQM −48.7 ± 4.5 11.4 ± 0.5 0.0921 0.0528  −66.2 ± 2.0 3.7 ± 1.0 0.0023 0.0136 
DII-IQM −32.4 ± 0.8 9.3 ± 0.3 0.0546 0.6054  −49.3 ± 0.8 4.1 ± 0.5 4.4 × 10−7 0.0547 
DIII-IQM −41.0 ± 2.3 10.8 ± 0.9 0.9502 0.0537  −59.0 ± 1.2 4.0 ± 0.6 1.5 × 10−5 1.9 × 10−4 
DIV-IQM −39.3 ± 2.6 11.1 ± 1.0 0.1367 0.0413  −58.8 ± 2.6 3.4 ± 0.7 0.0183 4.8 × 10−4 
DIII-N1325S −52.1 ± 1.0 10.2 ± 1.6 0.0059 0.2699  −79.9 ± 3.6 7.7 ± 0.7 0.2755 0.1921 
DIII-A1330P −51.1 ± 2.7 9.8 ± 0.6 0.0081 0.9331  −79.4 ± 3.9 11.6 ± 0.6 0.3619 1.4 × 10−7 
DIII-N1659A −37.6 ± 5.9 10.8 ± 0.5 0.5977 0.9349  −42.5 ± 1.4 5.0 ± 0.5 1.1 × 10−10 2.3 × 10−5 
DIV-N1659A −4.9 ± 1.1 11.4 ± 0.2 9.5 × 10−8 4.6 × 10−5  −48.6 ± 4.0 7.0 ± 0.5 0.0046 9.5 × 10−4 
FV and SSI-FV          
DI-LFS −100.4 ± 4.1 18.6 ± 1.0    −83.4 ± 2.6 15.6 ± 1.3   
DII-LFS −48.6 ± 3.3 20.9 ± 1.6    −48.8 ± 3.5 18.0 ± 0.9   
DIII-LFS −106.1 ± 3.2 21.7 ± 0.9    −113.6 ± 2.6 15.2 ± 0.6   
DIV-LFS −61.8 ± 3.6 16.9 ± 1.1    −75.0 ± 2.9 10.0 ± 1.3   
DI-IQM −91.7 ± 6.1 18.9 ± 3.4 0.2733 0.7766  −91.6 ± 4.2 26.1 ± 2.0 0.1189 0.0074 
DII-IQM −50.8 ± 5.0 29.2 ± 4.0 0.6618 0.0833  −57.2 ± 4.0 17.2 ± 3.4 0.2505 0.9400 
DIII-IQM −106.4 ± 5.2 21.7 ± 1.9 0.7518 0.4405  −92.6 ± 2.5 23.1 ± 1.5 0.0021 0.0014 
DIV-IQM −48.9 ± 3.5 10.8 ± 2.8 0.0335 0.1183  −59.2 ± 2.4 5.8 ± 1.2 0.0032 0.0299 
DIII-N1325S −125.1 ± 1.9 20.7 ± 0.7 2.6 × 10−4 0.1545  −106.3 ± 2.9 13.9 ± 0.7 0.1328 0.0644 
DIII-A1330P −123.8 ± 3.4 23.6 ± 1.5 0.0018 0.8074  −110.9 ± 2.8 15.8 ± 1.3 0.5992 0.9248 
DIII-N1659A −97.0 ± 7.5 24.9 ± 2.8 0.2059 0.6136  −85.0 ± 5.7 23.3 ± 1.5 0.0019 0.0015 
DIV-N1659A −44.3 ± 2.8 10.6 ± 1.1 0.0087 0.0089  −48.7 ± 2.3 14.2 ± 2.2 1.4 × 10−4 0.2343 
Channel variantGV/FVSSI/SSI-FV
V1/2kPVPkV1/2kPVPk
 mV mV    mV mV   
GV and SSI          
WT −35.3 ± 2.5 8.5 ± 0.5    −83.6 ± 1.6 7.3 ± 0.4   
WT-LFS −36.0 ± 2.1 8.6 ± 0.4    −88.5 ± 1.3 6.4 ± 0.2   
DI-LFS −57.7 ± 1.7 7.5 ± 1.4    −78.0 ± 1.4 6.5 ± 0.2   
DII-LFS −36.4 ± 1.7 8.7 ± 1.1    −82.0 ± 1.5 7.7 ± 1.0   
DIII-LFS −39.9 ± 2.2 9.5 ± 0.4    −76.2 ± 1.2 6.7 ± 0.2   
DIV-LFS −33.4 ± 2.5 9.3 ± 0.4    −71.4 ± 1.0 10.0 ± 0.6   
IQM-LFS −30.9 ± 2.0 9.2 ± 0.2 0.1428 0.2269  −52.0 ± 2.1 7.3 ± 1.9 3.8 × 10−6 0.1770 
A1330P −36.0 ± 1.9 7.7 ± 0.4 0.8062 0.2825  −74.6 ± 1.1 6.6 ± 0.5 0.0017 0.2597 
N1325S −42.5 ± 0.3 2.6 ± 0.7 0.0689 3.2 × 10−4  −68.3 ± 3.7 5.4 ± 0.2 0.0045 0.0102 
N1659A −23.7 ± 2.4 9.7 ± 0.3 0.0085 0.1639  −52.6 ± 1.5 7.5 ± 1.0 1.6 × 10−7 0.7790 
DI-IQM −48.7 ± 4.5 11.4 ± 0.5 0.0921 0.0528  −66.2 ± 2.0 3.7 ± 1.0 0.0023 0.0136 
DII-IQM −32.4 ± 0.8 9.3 ± 0.3 0.0546 0.6054  −49.3 ± 0.8 4.1 ± 0.5 4.4 × 10−7 0.0547 
DIII-IQM −41.0 ± 2.3 10.8 ± 0.9 0.9502 0.0537  −59.0 ± 1.2 4.0 ± 0.6 1.5 × 10−5 1.9 × 10−4 
DIV-IQM −39.3 ± 2.6 11.1 ± 1.0 0.1367 0.0413  −58.8 ± 2.6 3.4 ± 0.7 0.0183 4.8 × 10−4 
DIII-N1325S −52.1 ± 1.0 10.2 ± 1.6 0.0059 0.2699  −79.9 ± 3.6 7.7 ± 0.7 0.2755 0.1921 
DIII-A1330P −51.1 ± 2.7 9.8 ± 0.6 0.0081 0.9331  −79.4 ± 3.9 11.6 ± 0.6 0.3619 1.4 × 10−7 
DIII-N1659A −37.6 ± 5.9 10.8 ± 0.5 0.5977 0.9349  −42.5 ± 1.4 5.0 ± 0.5 1.1 × 10−10 2.3 × 10−5 
DIV-N1659A −4.9 ± 1.1 11.4 ± 0.2 9.5 × 10−8 4.6 × 10−5  −48.6 ± 4.0 7.0 ± 0.5 0.0046 9.5 × 10−4 
FV and SSI-FV          
DI-LFS −100.4 ± 4.1 18.6 ± 1.0    −83.4 ± 2.6 15.6 ± 1.3   
DII-LFS −48.6 ± 3.3 20.9 ± 1.6    −48.8 ± 3.5 18.0 ± 0.9   
DIII-LFS −106.1 ± 3.2 21.7 ± 0.9    −113.6 ± 2.6 15.2 ± 0.6   
DIV-LFS −61.8 ± 3.6 16.9 ± 1.1    −75.0 ± 2.9 10.0 ± 1.3   
DI-IQM −91.7 ± 6.1 18.9 ± 3.4 0.2733 0.7766  −91.6 ± 4.2 26.1 ± 2.0 0.1189 0.0074 
DII-IQM −50.8 ± 5.0 29.2 ± 4.0 0.6618 0.0833  −57.2 ± 4.0 17.2 ± 3.4 0.2505 0.9400 
DIII-IQM −106.4 ± 5.2 21.7 ± 1.9 0.7518 0.4405  −92.6 ± 2.5 23.1 ± 1.5 0.0021 0.0014 
DIV-IQM −48.9 ± 3.5 10.8 ± 2.8 0.0335 0.1183  −59.2 ± 2.4 5.8 ± 1.2 0.0032 0.0299 
DIII-N1325S −125.1 ± 1.9 20.7 ± 0.7 2.6 × 10−4 0.1545  −106.3 ± 2.9 13.9 ± 0.7 0.1328 0.0644 
DIII-A1330P −123.8 ± 3.4 23.6 ± 1.5 0.0018 0.8074  −110.9 ± 2.8 15.8 ± 1.3 0.5992 0.9248 
DIII-N1659A −97.0 ± 7.5 24.9 ± 2.8 0.2059 0.6136  −85.0 ± 5.7 23.3 ± 1.5 0.0019 0.0015 
DIV-N1659A −44.3 ± 2.8 10.6 ± 1.1 0.0087 0.0089  −48.7 ± 2.3 14.2 ± 2.2 1.4 × 10−4 0.2343 

Midpoints and slope factors from Boltzmann fits y/ymax = 1/(1 + exp[(V − V1/2)/k)] to steady-state activation and SSI data of ionic currents and fluorescence changes. Mean ± SEM of each parameter for sets of three to seven cells are shown. P-values from two sample t tests for mutants compare mutant parameters with corresponding WT parameters. Significant p-values <0.005 are in bold.

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