Table 3. Activation rate and equilibrium... | Rockefeller University Press

Table 3.

Activation rate and equilibrium constants for mutant AChRs

Mutant	k₊	k₋	K_d	J_d
	mM⁻¹s⁻¹	s⁻¹	M	M
GlyB2^a	2,484	2,501	1 E-3	0.95E-6
A^a	24,300	2,776	0.11 E-3	9.6 E-8
S^a	34,300	3,135	0.09 E-3	7.9 E-8
R^a	43,308	1,365	0.03 E-3	2.6 E-8
E^a	47,111	1,234	0.03 E-3	2.6 E-8
P^a	104,001	3,891	0.04 E-3	3.5 E-8
TrpD^b	106,108	17,614	0.16 E-3	2.65E-8
Y^b	37,357	10,460	0.28 E-3	4.65E-8
F^b	26,612	16,500	0.62 E-3	10.3 E-8
I^b	19,972	66,310	0.33 E-4	0.5 E-8
V^b	19,641	30,640	0.15 E-4	0.2 E-8
A^b	4,783	17,890	0.37 E-4	0.6 E-8

Mutant	k₊	k₋	K_d	J_d
	mM⁻¹s⁻¹	s⁻¹	M	M
GlyB2^a	2,484	2,501	1 E-3	0.95E-6
A^a	24,300	2,776	0.11 E-3	9.6 E-8
S^a	34,300	3,135	0.09 E-3	7.9 E-8
R^a	43,308	1,365	0.03 E-3	2.6 E-8
E^a	47,111	1,234	0.03 E-3	2.6 E-8
P^a	104,001	3,891	0.04 E-3	3.5 E-8
TrpD^b	106,108	17,614	0.16 E-3	2.65E-8
Y^b	37,357	10,460	0.28 E-3	4.65E-8
F^b	26,612	16,500	0.62 E-3	10.3 E-8
I^b	19,972	66,310	0.33 E-4	0.5 E-8
V^b	19,641	30,640	0.15 E-4	0.2 E-8
A^b	4,783	17,890	0.37 E-4	0.6 E-8

GlyB2 (αG153) mutants were activated by nicotine, and TrpD (εW55) mutants were activated by ACh.

Unpublished data.

Bafna et al., 2009.