[3H]Ouabain binding sites in soleus, EDL, and tibialis anterior muscles of WT and mutant mice
| Muscle | [3H]Ouabain binding | P-value |
| pmol/g wet wt | ||
| WT soleus | 746 ± 28 (6) | <0.004 |
| Mutant soleus | 952 ± 43 (10) | |
| WT EDL | 647 ± 14 (4) | <0.001 |
| Mutant EDL | 1,047 ± 37 (4) | |
| WT tibialis anterior | 417 ± 28 (8) | <0.001 |
| Mutant tibialis anterior | 554 ± 22 (8) |
| Muscle | [3H]Ouabain binding | P-value |
| pmol/g wet wt | ||
| WT soleus | 746 ± 28 (6) | <0.004 |
| Mutant soleus | 952 ± 43 (10) | |
| WT EDL | 647 ± 14 (4) | <0.001 |
| Mutant EDL | 1,047 ± 37 (4) | |
| WT tibialis anterior | 417 ± 28 (8) | <0.001 |
| Mutant tibialis anterior | 554 ± 22 (8) |
[3H]Ouabain binding in 12.5-wk-old WT and mutant mice. Whole frozen soleus and EDL muscles or biopsies of tibialis anterior muscles were incubated for 2 h at 37°C in Tris-vanadate buffer containing 10−6 M [3H]ouabain. After the incubation, the muscles were washed four times for 30 min in ice-cold Tris-vanadate buffer to remove unbound [3H]ouabain, blotted, and taken for counting and calculation of specific [3H]ouabain binding as described in the Materials and methods section.