Effects of 10−6 M salbutamol and 10−6 M rCGRP on total 86Rb uptake and total Na+ contents in WT and mutant mouse soleus
| Genotype | 86Rb uptake | P-value | Total Na+ content | P-value |
| nmol/g wet wt/min | µmol/g wet wt | |||
| WT | 697 ± 27 (6) | <0.02 | 42.6 ± 2.1 (6) | >0.5 |
| WT + salbutamol | 821 ± 36 (6) | 41.1 ± 0.9 (6) | ||
| Mutant | 783 ± 15 (6) | <0.005 | 49.5 ± 0.9 (6) | <0.001 |
| Mutant + salbutamol | 858 ± 14 (6) | 41.8 ± 1.3 (6) | ||
| WT | 650 ± 24 (4) | <0.02 | 45.1 ± 2.1 (4) | >0.15 |
| WT + rCGRP | 776 ± 28 (4) | 40.9 ± 1.6 (4) | ||
| Mutant | 815 ± 17 (8) | >0.9 | 45.4 ± 1.4 (8) | >0.08 |
| Mutant + rCGRP | 818 ± 30 (8) | 41.9 ± 1.4 (8) |
| Genotype | 86Rb uptake | P-value | Total Na+ content | P-value |
| nmol/g wet wt/min | µmol/g wet wt | |||
| WT | 697 ± 27 (6) | <0.02 | 42.6 ± 2.1 (6) | >0.5 |
| WT + salbutamol | 821 ± 36 (6) | 41.1 ± 0.9 (6) | ||
| Mutant | 783 ± 15 (6) | <0.005 | 49.5 ± 0.9 (6) | <0.001 |
| Mutant + salbutamol | 858 ± 14 (6) | 41.8 ± 1.3 (6) | ||
| WT | 650 ± 24 (4) | <0.02 | 45.1 ± 2.1 (4) | >0.15 |
| WT + rCGRP | 776 ± 28 (4) | 40.9 ± 1.6 (4) | ||
| Mutant | 815 ± 17 (8) | >0.9 | 45.4 ± 1.4 (8) | >0.08 |
| Mutant + rCGRP | 818 ± 30 (8) | 41.9 ± 1.4 (8) |
The muscles were mounted at resting length on stimulation electrodes, equilibrated for 30 min in KR with 10−5 M tubocurarine, transferred to KR with 0.2 µCi/ml 86Rb, incubated for 40 min with or without 10−6 M salbutamol or 10−6 M rCGRP, blotted, weighed, and taken for counting of 86Rb and measurement of Na+ content. All muscles were stimulated every 10 min with 2-s 120-Hz trains of 0.2-ms pulses of 7 V. All values are given as means with SEM and the number of muscles in parentheses. Controls are compared with contralateral muscles exposed to salbutamol or to rCGRP.