Parameters of chimeras and mutants for the KCNE1 study
| Chimera/mutant name | Without KCNE1 | With KCNE1 | ||||||
| n | I (μA) | V1/2 (mV) | z | n | I (μA) | V1/2 (mV) | z | |
| Human KCNQ1 | 29 | 0.4 ± 0.0 | −22.7 ± 0.7 | 2.8 ± 0.1 | 24 | 6.1 ± 0.7 | 35.2 ± 2.1 | 1.4 ± 0.0 |
| Ci-KCNQ1 | 15 | 3.9 ± 0.4 | −4.2 ± 0.9 | 3.1 ± 0.1 | 7 | 6.5 ± 1.1 | −29.4 ± 2.1 | 1.9 ± 0.1 |
| Chimeras | ||||||||
| hQ1ciQ1(C-term) | 5 | 0.8 ± 0.2 | −15.7 ± 0.8 | 2.7 ± 0.0 | 5 | 7.8 ± 1.5 | 43.8 ± 1.6 | 1.3 ± 0.0 |
| ciQ1(N-S3)hQ1(S4-C) | 6 | 1.0 ± 0.6 | −25.4 ± 1.1 | 2.4 ± 0.1 | 10 | 7.9 ± 1.5 | 29.8 ± 2.1 | 1.3 ± 0.0 |
| ciQ1hQ1(S4-S6) | 5 | 0.9 ± 0.2 | −17.1 ± 0.7 | 2.3 ± 0.0 | 5 | 8.0 ± 1.5 | 44.1 ± 3.5 | 1.5 ± 0.0 |
| ciQ1hQ1(S5P-S6) | 19 | 3.6 ± 0.3 | −12.1 ± 0.4 | 2.9 ± 0.0 | 18 | 11.0 ± 0.9 | −8.3 ± 2.0 | 1.7 ± 0.1 |
| ciQ1hQ1(S4-S5P) | 3 | 1.5 ± 1.0 | −18.1 ± 0.3 | 2.9 ± 0.2 | 6 | 7.6 ± 2.1 | −5.8 ± 4.7 | 1.7 ± 0.2 |
| ciQ1hQ1(S5P) | 13 | 4.2 ± 0.3 | −19.8 ± 0.3 | 3.0 ± 0.1 | 16 | 10.7 ± 1.1 | −30.0 ± 1.1 | 2.1 ± 0.1 |
| ciQ1hQ1(286-297) mutants | ||||||||
| Y175F | 8 | 5.0 ± 0.6 | −17.6 ± 0.5 | 3.2 ± 0.1 | 15 | 12.0 ± 0.9 | −21.0 ± 1.1 | 2.0 ± 0.1 |
| A191G | 18 | 2.6 ± 0.5 | −6.3 ± 1.4 | 2.8 ± 0.0 | 21 | 8.1 ± 0.6 | −2.2 ± 0.8 | 1.8 ± 0.0 |
| H242K/I245Q | 8 | 1.5 ± 0.2 | −16.0 ± 0.9 | 3.2 ± 0.1 | 8 | 5.3 ± 1.1 | −27.7 ± 2.9 | 1.9 ± 0.1 |
| L248V | 18 | 1.2 ± 0.1 | −17.1 ± 0.4 | 3.0 ± 0.0 | 18 | 5.2 ± 0.5 | 13.7 ± 2.5 | 1.4 ± 0.0 |
| V251T | 9 | 2.5 ± 0.3 | −21.6 ± 0.7 | 3.2 ± 0.1 | 9 | 7.0 ± 2.1 | −15.5 ± 2.7 | 1.7 ± 0.0 |
| I258V | 17 | 1.4 ± 0.1 | −19.9 ± 0.6 | 3.0 ± 0.1 | 17 | 5.9 ± 0.4 | −0.5 ± 2.5 | 1.5 ± 0.0 |
| A191G/L248V | 15 | 4.4 ± 0.5 | −9.5 ± 0.7 | 2.5 ± 0.1 | 19 | 10.6 ± 0.9 | 8.4 ± 2.9 | 1.7 ± 0.1 |
| A191G/I258V | 17 | 1.5 ± 0.2 | −17.3 ± 0.6 | 2.6 ± 0.1 | 17 | 6.9 ± 0.4 | 31.0 ± 2.6 | 1.3 ± 0.0 |
| L248V/I258V | 19 | 4.1 ± 0.6 | −16.0 ± 0.6 | 3.0 ± 0.1 | 19 | 15.6 ± 1.1 | 3.6 ± 1.1 | 1.6 ± 0.0 |
| A191G/L248V/I258V | 19 | 2.4 ± 0.5 | −9.6 ± 0.8 | 2.5 ± 0.0 | 19 | 14.4 ± 1.3 | 31.9 ± 2.4 | 1.3 ± 0.0 |
| Ci-KCNQ1 mutants | ||||||||
| A191G | 10 | 0.2 ± 0.0 | −1.4 ± 0.6 | 3.4 ± 0.2 | 10 | 2.3 ± 0.5 | −12.6 ± 1.5 | 2.5 ± 0.1 |
| L248V | 10 | 4.8 ± 0.6 | 1.2 ± 0.7 | 2.8 ± 0.1 | 10 | 5.9 ± 0.7 | −14.7 ± 1.3 | 2.4 ± 0.1 |
| I258V | 10 | 2.9 ± 0.4 | −6.8 ± 0.6 | 3.2 ± 0.1 | 10 | 6.7 ± 1.0 | −18.9 ± 1.4 | 2.6 ± 0.1 |
| hKCNQ1 mutants | ||||||||
| F256Y | 6 | 0.6 ± 0.1 | −27.0 ± 0.5 | 2.5 ± 0.1 | 6 | 11.5 ± 1.0 | 27.3 ± 1.7 | 1.4 ± 0.0 |
| G272A | 15 | 1.3 ± 0.2 | −19.6 ± 0.4 | 3.6 ± 0.1 | 18 | 8.8 ± 1.1 | 10.4 ± 2.5 | 1.5 ± 0.1 |
| V324L | 10 | 0.6 ± 0.1 | −29.5 ± 0.7 | 3.0 ± 0.1 | 10 | 12.9 ± 1.4 | 17.3 ± 1.9 | 1.6 ± 0.1 |
| T327V | 6 | 0.3 ± 0.1 | −28.5 ± 0.9 | 2.4 ± 0.0 | 6 | 8.6 ± 0.4 | 37.9 ± 2.0 | 1.4 ± 0.0 |
| V334I | 10 | 2.5 ± 0.2 | −18.8 ± 0.5 | 3.1 ± 0.1 | 10 | 16.8 ± 1.4 | 3.6 ± 2.5 | 1.5 ± 0.1 |
| G272A/I334L | 6 | 1.6 ± 0.2 | −17.4 ± 0.3 | 2.9 ± 0.1 | 6 | 15.6 ± 1.8 | −0.1 ± 1.8 | 1.0 ± 0.1 |
| Chimera/mutant name | Without KCNE1 | With KCNE1 | ||||||
| n | I (μA) | V1/2 (mV) | z | n | I (μA) | V1/2 (mV) | z | |
| Human KCNQ1 | 29 | 0.4 ± 0.0 | −22.7 ± 0.7 | 2.8 ± 0.1 | 24 | 6.1 ± 0.7 | 35.2 ± 2.1 | 1.4 ± 0.0 |
| Ci-KCNQ1 | 15 | 3.9 ± 0.4 | −4.2 ± 0.9 | 3.1 ± 0.1 | 7 | 6.5 ± 1.1 | −29.4 ± 2.1 | 1.9 ± 0.1 |
| Chimeras | ||||||||
| hQ1ciQ1(C-term) | 5 | 0.8 ± 0.2 | −15.7 ± 0.8 | 2.7 ± 0.0 | 5 | 7.8 ± 1.5 | 43.8 ± 1.6 | 1.3 ± 0.0 |
| ciQ1(N-S3)hQ1(S4-C) | 6 | 1.0 ± 0.6 | −25.4 ± 1.1 | 2.4 ± 0.1 | 10 | 7.9 ± 1.5 | 29.8 ± 2.1 | 1.3 ± 0.0 |
| ciQ1hQ1(S4-S6) | 5 | 0.9 ± 0.2 | −17.1 ± 0.7 | 2.3 ± 0.0 | 5 | 8.0 ± 1.5 | 44.1 ± 3.5 | 1.5 ± 0.0 |
| ciQ1hQ1(S5P-S6) | 19 | 3.6 ± 0.3 | −12.1 ± 0.4 | 2.9 ± 0.0 | 18 | 11.0 ± 0.9 | −8.3 ± 2.0 | 1.7 ± 0.1 |
| ciQ1hQ1(S4-S5P) | 3 | 1.5 ± 1.0 | −18.1 ± 0.3 | 2.9 ± 0.2 | 6 | 7.6 ± 2.1 | −5.8 ± 4.7 | 1.7 ± 0.2 |
| ciQ1hQ1(S5P) | 13 | 4.2 ± 0.3 | −19.8 ± 0.3 | 3.0 ± 0.1 | 16 | 10.7 ± 1.1 | −30.0 ± 1.1 | 2.1 ± 0.1 |
| ciQ1hQ1(286-297) mutants | ||||||||
| Y175F | 8 | 5.0 ± 0.6 | −17.6 ± 0.5 | 3.2 ± 0.1 | 15 | 12.0 ± 0.9 | −21.0 ± 1.1 | 2.0 ± 0.1 |
| A191G | 18 | 2.6 ± 0.5 | −6.3 ± 1.4 | 2.8 ± 0.0 | 21 | 8.1 ± 0.6 | −2.2 ± 0.8 | 1.8 ± 0.0 |
| H242K/I245Q | 8 | 1.5 ± 0.2 | −16.0 ± 0.9 | 3.2 ± 0.1 | 8 | 5.3 ± 1.1 | −27.7 ± 2.9 | 1.9 ± 0.1 |
| L248V | 18 | 1.2 ± 0.1 | −17.1 ± 0.4 | 3.0 ± 0.0 | 18 | 5.2 ± 0.5 | 13.7 ± 2.5 | 1.4 ± 0.0 |
| V251T | 9 | 2.5 ± 0.3 | −21.6 ± 0.7 | 3.2 ± 0.1 | 9 | 7.0 ± 2.1 | −15.5 ± 2.7 | 1.7 ± 0.0 |
| I258V | 17 | 1.4 ± 0.1 | −19.9 ± 0.6 | 3.0 ± 0.1 | 17 | 5.9 ± 0.4 | −0.5 ± 2.5 | 1.5 ± 0.0 |
| A191G/L248V | 15 | 4.4 ± 0.5 | −9.5 ± 0.7 | 2.5 ± 0.1 | 19 | 10.6 ± 0.9 | 8.4 ± 2.9 | 1.7 ± 0.1 |
| A191G/I258V | 17 | 1.5 ± 0.2 | −17.3 ± 0.6 | 2.6 ± 0.1 | 17 | 6.9 ± 0.4 | 31.0 ± 2.6 | 1.3 ± 0.0 |
| L248V/I258V | 19 | 4.1 ± 0.6 | −16.0 ± 0.6 | 3.0 ± 0.1 | 19 | 15.6 ± 1.1 | 3.6 ± 1.1 | 1.6 ± 0.0 |
| A191G/L248V/I258V | 19 | 2.4 ± 0.5 | −9.6 ± 0.8 | 2.5 ± 0.0 | 19 | 14.4 ± 1.3 | 31.9 ± 2.4 | 1.3 ± 0.0 |
| Ci-KCNQ1 mutants | ||||||||
| A191G | 10 | 0.2 ± 0.0 | −1.4 ± 0.6 | 3.4 ± 0.2 | 10 | 2.3 ± 0.5 | −12.6 ± 1.5 | 2.5 ± 0.1 |
| L248V | 10 | 4.8 ± 0.6 | 1.2 ± 0.7 | 2.8 ± 0.1 | 10 | 5.9 ± 0.7 | −14.7 ± 1.3 | 2.4 ± 0.1 |
| I258V | 10 | 2.9 ± 0.4 | −6.8 ± 0.6 | 3.2 ± 0.1 | 10 | 6.7 ± 1.0 | −18.9 ± 1.4 | 2.6 ± 0.1 |
| hKCNQ1 mutants | ||||||||
| F256Y | 6 | 0.6 ± 0.1 | −27.0 ± 0.5 | 2.5 ± 0.1 | 6 | 11.5 ± 1.0 | 27.3 ± 1.7 | 1.4 ± 0.0 |
| G272A | 15 | 1.3 ± 0.2 | −19.6 ± 0.4 | 3.6 ± 0.1 | 18 | 8.8 ± 1.1 | 10.4 ± 2.5 | 1.5 ± 0.1 |
| V324L | 10 | 0.6 ± 0.1 | −29.5 ± 0.7 | 3.0 ± 0.1 | 10 | 12.9 ± 1.4 | 17.3 ± 1.9 | 1.6 ± 0.1 |
| T327V | 6 | 0.3 ± 0.1 | −28.5 ± 0.9 | 2.4 ± 0.0 | 6 | 8.6 ± 0.4 | 37.9 ± 2.0 | 1.4 ± 0.0 |
| V334I | 10 | 2.5 ± 0.2 | −18.8 ± 0.5 | 3.1 ± 0.1 | 10 | 16.8 ± 1.4 | 3.6 ± 2.5 | 1.5 ± 0.1 |
| G272A/I334L | 6 | 1.6 ± 0.2 | −17.4 ± 0.3 | 2.9 ± 0.1 | 6 | 15.6 ± 1.8 | −0.1 ± 1.8 | 1.0 ± 0.1 |
Number of experiments (n), average maximum tail current amplitudes (μA), and parameters deduced from the Boltzmann fitting for the chimeras and pore mutants of Ciona and human KCNQ1.