Boltzmann fits to Kv1.2 and Shaker G-V and F-V relationships from Fig. 3
| Kv1.2 | Shaker | ||||||||
| Construct | G-V | F-V | Construct | G-V | F-V | ||||
| V1/2 | k | V1/2 | k | V1/2 | k | V1/2 | k | ||
| mV | mV | mV | mV | mV | mV | mV | mV | ||
| M288C | −12.4 ± 1.1 | 23.0 ± 0.4 | −56.9 ± 3.5 | 26.2 ± 1.6 | M356C | −0.5 ± 1.5 | 17.3 ± 0.7 | −41.8 ± 2.6 | 20.0 ± 1.7 |
| S289C (1) | 9.8 ± 3.3 | 18.1 ± 0.7 | −105.0 ± 2.8 | −9.3 ± 2.6 | S357C | 5.6 ± 2.3 | 18.6 ± 1.1 | −42.9 ± 4.4 | 9.7 ± 1.2 |
| S289C (2) | n/a | n/a | 41.7 ± 9.2 | −33.3 ± 2.0 | − | n/a | n/a | n/a | n/a |
| L290C | −39.3 ± 4.7 | 26.5 ± 4.7 | −91.9 ± 2.4 | 19.7 ± 1.7 | L358C | −26.2 ± 4.2 | 18.2 ± 1.8 | −59.5 ± 0.5 | 21.6 ± 1.7 |
| A291C | −12.6 ± 1.9 | 26.1 ± 1.4 | −62.5 ± 1.3 | 23.3 ± 1.0 | A359C | −14.6 ± 1.7 | 19.8 ± 1.4 | −38.8 ± 1.9 | 22.6 ± 1.4 |
| Kv1.2 | Shaker | ||||||||
| Construct | G-V | F-V | Construct | G-V | F-V | ||||
| V1/2 | k | V1/2 | k | V1/2 | k | V1/2 | k | ||
| mV | mV | mV | mV | mV | mV | mV | mV | ||
| M288C | −12.4 ± 1.1 | 23.0 ± 0.4 | −56.9 ± 3.5 | 26.2 ± 1.6 | M356C | −0.5 ± 1.5 | 17.3 ± 0.7 | −41.8 ± 2.6 | 20.0 ± 1.7 |
| S289C (1) | 9.8 ± 3.3 | 18.1 ± 0.7 | −105.0 ± 2.8 | −9.3 ± 2.6 | S357C | 5.6 ± 2.3 | 18.6 ± 1.1 | −42.9 ± 4.4 | 9.7 ± 1.2 |
| S289C (2) | n/a | n/a | 41.7 ± 9.2 | −33.3 ± 2.0 | − | n/a | n/a | n/a | n/a |
| L290C | −39.3 ± 4.7 | 26.5 ± 4.7 | −91.9 ± 2.4 | 19.7 ± 1.7 | L358C | −26.2 ± 4.2 | 18.2 ± 1.8 | −59.5 ± 0.5 | 21.6 ± 1.7 |
| A291C | −12.6 ± 1.9 | 26.1 ± 1.4 | −62.5 ± 1.3 | 23.3 ± 1.0 | A359C | −14.6 ± 1.7 | 19.8 ± 1.4 | −38.8 ± 1.9 | 22.6 ± 1.4 |
n/a, not applicable. Mean half-activation (V1/2) and slope (k) data were calculated from single Boltzmann fits to the data, with the exception of the F-V relationship for S289C, which was best fit with a double Boltzmann function.