Table II.

Single-channel parameters of wild-type and Kir6.2-G334D/SUR1 channels in nucleotide-free solution

Channel τC1 aC1 τC2 aC2 τC3 aC3 τC4 aC4 
 ms  ms  ms  ms  
Wild-type 0.40 ± 0.02 0.923 ± 0.014 15.2 ± 3.3 0.025 ± 0.003 114 ± 41 0.045 ± 0.014 253 ± 55 0.007 ± 0.002 
Kir6.2-G334D/SUR1 0.40 ± 0.02 0.943 ± 0.024 12.5 ± 2.4 0.019 ± 0.007 72 ± 34 0.026 ± 0.008 299 ± 88 0.010 ± 0.004 
Channel τC1 aC1 τC2 aC2 τC3 aC3 τC4 aC4 
 ms  ms  ms  ms  
Wild-type 0.40 ± 0.02 0.923 ± 0.014 15.2 ± 3.3 0.025 ± 0.003 114 ± 41 0.045 ± 0.014 253 ± 55 0.007 ± 0.002 
Kir6.2-G334D/SUR1 0.40 ± 0.02 0.943 ± 0.024 12.5 ± 2.4 0.019 ± 0.007 72 ± 34 0.026 ± 0.008 299 ± 88 0.010 ± 0.004 

τCi, ai, closed times and their corresponding amplitudes in the distribution of closed times (i =1–4). Data are expressed as mean ± SEM (n = 3 each).

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