Single-channel parameters of wild-type and Kir6.2-G334D/SUR1 channels in nucleotide-free solution
| Channel | PO(0) | τO | τO,COR | τB1 | aB1 | τB2 | aB2 | τB3 | aB3 | τB |
| ms | ms | ms | ms | ms | ms | |||||
| Wild-type | 0.37 ± 0.07 | 2.9 ± 0.3 | 2.1 ± 0.2 | 77 ± 19 | 0.55 ± 0.05 | 28 ± 6 | 0.30 ± 0.04 | 1.4 ± 0.6 | 0.12 ± 0.01 | 52 ± 11 |
| Kir6.2-G334D/SUR1 | 0.34 ± 0.04 | 2.8 ± 0.3 | 1.9 ± 0.2 | 63 ± 10 | 0.69 ± 0.08 | 17 ± 5 | 0.21 ± 0.06 | 1.2 ± 0.4 | 0.13 ± 0.02 | 45 ± 5 |
| Channel | PO(0) | τO | τO,COR | τB1 | aB1 | τB2 | aB2 | τB3 | aB3 | τB |
| ms | ms | ms | ms | ms | ms | |||||
| Wild-type | 0.37 ± 0.07 | 2.9 ± 0.3 | 2.1 ± 0.2 | 77 ± 19 | 0.55 ± 0.05 | 28 ± 6 | 0.30 ± 0.04 | 1.4 ± 0.6 | 0.12 ± 0.01 | 52 ± 11 |
| Kir6.2-G334D/SUR1 | 0.34 ± 0.04 | 2.8 ± 0.3 | 1.9 ± 0.2 | 63 ± 10 | 0.69 ± 0.08 | 17 ± 5 | 0.21 ± 0.06 | 1.2 ± 0.4 | 0.13 ± 0.02 | 45 ± 5 |
PO(0), intrinsic (ligand-free) single-channel open probability; τO, mean open time; τO,COR, mean open time corrected for missed events (Proks et al., 2001); τBi and aBi, burst durations and their corresponding amplitudes in the distribution of burst lengths (i =1–3); τB, mean burst duration. Data are expressed as mean ± SEM (n = 3 each).