Response properties of rod bipolar cells and AIIACs in different mouse lines
| Rod bipolar | ||||||
| Gαo+/+ | Gαo+/− | Gαo−/− | Gαo1+/+ | Gαo1+/− | Gαo1−/− | |
| Imax (pA) | 420 ± 38 (14)b | 250 ± 22(15)bd | −0.3 ± 0.3 (10)d | 490 ± 36 (16)b | 350 ± 28(17)bd | 5.3 ± 0.8 (9)d |
| Idark (pA) | −33 ± 4.2 (14) | −28 ± 3.3 (15) | −27 ± 2.2 (10) | −25 ± 3.0 (16) | −27 ± 2.4 (17) | −33 ± 5.0 (9) |
| σ2 (pA2) | 14 ± 3.1 (13)a | 6.4 ± 1.6 (15)ac | 2.2 ± 0.3 (10)c | 9.6 ± 1.3 (14) | 12 ± 2.0 (16)c | 4.7 ± 0.6 (9)c |
| I1/2 (Rh*/rod) | 2.5 ± 0.13 (14) | 2.5 ± 0.17 (15) | 2.8 ± 0.12 (16) | 2.5 ± 0.15 (17) | ||
| n | 1.5 ± 0.04 (14) | 1.6 ± 0.06 (15) | 1.4 ± 0.05 (16) | 1.5 ± 0.07 (17) | ||
| τint (ms) | 120 ± 10 (9.1) | 100 ± 8 (10.0) | 110 ± 6 (11.7) | 120 ± 6 (10.3) | ||
| Tpeak (ms) | 120 ± 4 (9.1)a | 130 ± 5 (10.0)a | 120 ± 3 (11.7) | 120 ± 4 (10.3) | ||
| % Isat | 16 ± 1.2 (9.1) | 15 ± 2.0 (10.0) | 16 ± 1.4 (11.7) | 16 ± 1.7 (10.3) | ||
| Rod bipolar | AIIAC | |||||
| Gαo2+/+ | Gαo2−/− | Gαo1+/+ Cx36−/− | Gαo1−/− Cx36−/− | |||
| Imax (pA) | 430 ± 44 (15) | 370 ± 37 (16) | 210 ± 44 (10)a | 100 ± 22 (9)a | ||
| Idark (pA) | −30 ± 4.3 (16) | −29 ± 2.7 (16) | ||||
| σ2 (pA2) | 12 ± 2.0 (14) | 13 ± 1.8 (16) | ||||
| I1/2 (Rh*/rod) | 2.2 ± 0.15 (15)a | 2.6 ± 0.19 (16)a | 0.17 ± 0.01(10)b | 2.6 ± 0.13 (9)b | ||
| n | 1.5 ± 0.02 (15) | 1.6 ± 0.05 (16) | 1.6 ± 0.09 (10) | 1.5 ± 0.09 (9) | ||
| τint (ms) | 120 ± 7 (9.2) | 130 ± 11 (8.0) | ||||
| Tpeak (ms) | 120 ± 4 (9.2) | 130 ± 8 (8.0) | ||||
| % Isat | 19 ± 1.8 (9.2)a | 14 ± 1.6 (8.0)a | ||||
| Rod bipolar | ||||||
| Gαo+/+ | Gαo+/− | Gαo−/− | Gαo1+/+ | Gαo1+/− | Gαo1−/− | |
| Imax (pA) | 420 ± 38 (14)b | 250 ± 22(15)bd | −0.3 ± 0.3 (10)d | 490 ± 36 (16)b | 350 ± 28(17)bd | 5.3 ± 0.8 (9)d |
| Idark (pA) | −33 ± 4.2 (14) | −28 ± 3.3 (15) | −27 ± 2.2 (10) | −25 ± 3.0 (16) | −27 ± 2.4 (17) | −33 ± 5.0 (9) |
| σ2 (pA2) | 14 ± 3.1 (13)a | 6.4 ± 1.6 (15)ac | 2.2 ± 0.3 (10)c | 9.6 ± 1.3 (14) | 12 ± 2.0 (16)c | 4.7 ± 0.6 (9)c |
| I1/2 (Rh*/rod) | 2.5 ± 0.13 (14) | 2.5 ± 0.17 (15) | 2.8 ± 0.12 (16) | 2.5 ± 0.15 (17) | ||
| n | 1.5 ± 0.04 (14) | 1.6 ± 0.06 (15) | 1.4 ± 0.05 (16) | 1.5 ± 0.07 (17) | ||
| τint (ms) | 120 ± 10 (9.1) | 100 ± 8 (10.0) | 110 ± 6 (11.7) | 120 ± 6 (10.3) | ||
| Tpeak (ms) | 120 ± 4 (9.1)a | 130 ± 5 (10.0)a | 120 ± 3 (11.7) | 120 ± 4 (10.3) | ||
| % Isat | 16 ± 1.2 (9.1) | 15 ± 2.0 (10.0) | 16 ± 1.4 (11.7) | 16 ± 1.7 (10.3) | ||
| Rod bipolar | AIIAC | |||||
| Gαo2+/+ | Gαo2−/− | Gαo1+/+ Cx36−/− | Gαo1−/− Cx36−/− | |||
| Imax (pA) | 430 ± 44 (15) | 370 ± 37 (16) | 210 ± 44 (10)a | 100 ± 22 (9)a | ||
| Idark (pA) | −30 ± 4.3 (16) | −29 ± 2.7 (16) | ||||
| σ2 (pA2) | 12 ± 2.0 (14) | 13 ± 1.8 (16) | ||||
| I1/2 (Rh*/rod) | 2.2 ± 0.15 (15)a | 2.6 ± 0.19 (16)a | 0.17 ± 0.01(10)b | 2.6 ± 0.13 (9)b | ||
| n | 1.5 ± 0.02 (15) | 1.6 ± 0.05 (16) | 1.6 ± 0.09 (10) | 1.5 ± 0.09 (9) | ||
| τint (ms) | 120 ± 7 (9.2) | 130 ± 11 (8.0) | ||||
| Tpeak (ms) | 120 ± 4 (9.2) | 130 ± 8 (8.0) | ||||
| % Isat | 19 ± 1.8 (9.2)a | 14 ± 1.6 (8.0)a | ||||
All the values are given as mean ± SEM (n). The effective number of cells was used to calculate the SEM of τint, Tpeak, and % Isat (see Sampath et al., 2005). Imax is the maximal response amplitude. Idark and σ2 are the mean and the variance of holding current measured in the first 5 s after the establishment of whole-cell configuration. I1/2 is the half-maximal flash strength. n is the exponent in the Hill Equation fit to flash strength vs. normalized response amplitude curves. τint and Tpeak are the integration time and the time-to-peak of dim flash responses. % Isat is the fractional amplitude of a dim flash response to an average flash strength of 1 Rh*/rod.
P < 0.05, significant difference between littermates.
P < 0.01, significant difference between littermates.
P < 0.05, significant difference between Gαo−/− or Gαo1−/− rod bipolar cells compared to their heterozygote.
P < 0.01, significant difference between Gαo−/− or Gαo1−/− rod bipolar cells compared to their heterozygote.