Exponential fits comparing the activation and deactivation kinetics of KCa3.1
| Channel | [Ca2+]i | On | Off | n | PCMBS | ||
| On | Off | n | |||||
| μM | ms | ms | ms | ms | |||
| KCa3.1 (paired) | 0.5 | 224 ± 22 | 61 ± 5 | 13 | 337 ± 29 | 402 ± 31 | 13 |
| 0.7 | 185 ± 11 | 60 ± 5 | 13 | 250 ± 14 | 414 ± 30 | 13 | |
| 1.0 | 147 ± 9 | 65 ± 4 | 13 | 224 ± 12 | 428 ± 30 | 13 | |
| 10 | 123 ± 11 | 59 ± 4 | 13 | 206 ± 13 | 411 ± 34 | 13 | |
| KCa3.1 C276A | 0.5 | 211 ± 20 | 48 ± 4 | 7 | 191 ± 12 | 60 ± 8 | 7 |
| 0.7 | 186 ± 15 | 46 ± 4 | 7 | 154 ± 13 | 57 ± 6 | 7 | |
| 1.0 | 142 ± 15 | 56 ± 3 | 7 | 134 ± 12 | 61 ± 6 | 7 | |
| 10 | 120 ± 11 | 43 ± 3 | 7 | 103 ± 6 | 59 ± 6 | 7 | |
| KCa3.1 C277A | 0.5 | 244 ± 10 | 54 ± 6 | 6 | 318 ± 20 | 377 ± 45 | 5 |
| 0.7 | 186 ± 9 | 59 ± 6 | 6 | 262 ± 18 | 336 ± 46 | 5 | |
| 1.0 | 152 ± 8 | 59 ± 5 | 6 | 223 ± 19 | 365 ± 44 | 5 | |
| 10 | 129 ± 12 | 59 ± 7 | 6 | 194 ± 14 | 332 ± 39 | 5 | |
| KCa3.1 | 0.5 | 209 ± 9 | 57 ± 2 | 51 | |||
| 0.7 | 170 ± 5 | 57 ± 2 | 51 | ||||
| 1.0 | 141 ± 5 | 59 ± 2 | 51 | ||||
| 10 | 114 ± 5 | 56 ± 2 | 51 | ||||
| KCa3.1 L281W | 0.5 | 149 ± 12 | 186 ± 11 | 23 | 175 ± 20 | 267 ± 20 | 8 |
| 0.7 | 130 ± 9 | 202 ± 11 | 23 | 142 ± 17 | 278 ± 24 | 8 | |
| 1.0 | 119 ± 10 | 198 ± 9 | 23 | 119 ± 13 | 293 ± 27 | 8 | |
| 10 | 92 ± 7 | 197 ± 9 | 23 | 100 ± 10 | 288 ± 27 | 8 | |
| KCa3.1 V282W | 0.5 | 161 ± 11 | 59 ± 3 | 13 | |||
| 0.7 | 131 ± 8 | 59 ± 2 | 13 | ||||
| 1.0 | 113 ± 8 | 59 ± 3 | 13 | ||||
| 10 | 84 ± 5 | 58 ± 3 | 13 | ||||
| Channel | [Ca2+]i | On | Off | n | PCMBS | ||
| On | Off | n | |||||
| μM | ms | ms | ms | ms | |||
| KCa3.1 (paired) | 0.5 | 224 ± 22 | 61 ± 5 | 13 | 337 ± 29 | 402 ± 31 | 13 |
| 0.7 | 185 ± 11 | 60 ± 5 | 13 | 250 ± 14 | 414 ± 30 | 13 | |
| 1.0 | 147 ± 9 | 65 ± 4 | 13 | 224 ± 12 | 428 ± 30 | 13 | |
| 10 | 123 ± 11 | 59 ± 4 | 13 | 206 ± 13 | 411 ± 34 | 13 | |
| KCa3.1 C276A | 0.5 | 211 ± 20 | 48 ± 4 | 7 | 191 ± 12 | 60 ± 8 | 7 |
| 0.7 | 186 ± 15 | 46 ± 4 | 7 | 154 ± 13 | 57 ± 6 | 7 | |
| 1.0 | 142 ± 15 | 56 ± 3 | 7 | 134 ± 12 | 61 ± 6 | 7 | |
| 10 | 120 ± 11 | 43 ± 3 | 7 | 103 ± 6 | 59 ± 6 | 7 | |
| KCa3.1 C277A | 0.5 | 244 ± 10 | 54 ± 6 | 6 | 318 ± 20 | 377 ± 45 | 5 |
| 0.7 | 186 ± 9 | 59 ± 6 | 6 | 262 ± 18 | 336 ± 46 | 5 | |
| 1.0 | 152 ± 8 | 59 ± 5 | 6 | 223 ± 19 | 365 ± 44 | 5 | |
| 10 | 129 ± 12 | 59 ± 7 | 6 | 194 ± 14 | 332 ± 39 | 5 | |
| KCa3.1 | 0.5 | 209 ± 9 | 57 ± 2 | 51 | |||
| 0.7 | 170 ± 5 | 57 ± 2 | 51 | ||||
| 1.0 | 141 ± 5 | 59 ± 2 | 51 | ||||
| 10 | 114 ± 5 | 56 ± 2 | 51 | ||||
| KCa3.1 L281W | 0.5 | 149 ± 12 | 186 ± 11 | 23 | 175 ± 20 | 267 ± 20 | 8 |
| 0.7 | 130 ± 9 | 202 ± 11 | 23 | 142 ± 17 | 278 ± 24 | 8 | |
| 1.0 | 119 ± 10 | 198 ± 9 | 23 | 119 ± 13 | 293 ± 27 | 8 | |
| 10 | 92 ± 7 | 197 ± 9 | 23 | 100 ± 10 | 288 ± 27 | 8 | |
| KCa3.1 V282W | 0.5 | 161 ± 11 | 59 ± 3 | 13 | |||
| 0.7 | 131 ± 8 | 59 ± 2 | 13 | ||||
| 1.0 | 113 ± 8 | 59 ± 3 | 13 | ||||
| 10 | 84 ± 5 | 58 ± 3 | 13 | ||||
Time constants (τ) from single exponential fits to channel activation or deactivation records from the four [Ca2+]i concentrations examined.