Summary of the values of passive force, maximal active force, pCa50, and nH in PLV under various conditions
| SL | Passive force | Maximal force | pCa50 | ΔpCa50 | nH | |
| µm | mN/mm2 | mN/mm2 | ||||
| Without sTn reconstitution | ||||||
| Control | 1.9 | ∼0 | 57.25 ± 2.29 | 5.56 ± 0.01 | 3.86 ± 0.13 | |
| 2.3 | 9.40 ± 0.50 | 73.79 ± 3.72 | 5.80 ± 0.01 | 0.24 ± 0.01 | 3.17 ± 0.24 | |
| +ADP | 1.9 | ∼0 | 67.19 ± 1.39a (78.41 ± 2.97) | 5.82 ± 0.01b | 3.44 ± 0.10b | |
| 2.3 | 8.10 ± 0.67 | 75.61 ± 2.64 | 5.93 ± 0.01b | 0.10 ± 0.01b | 3.06 ± 0.16 | |
| +Pi | 1.9 | ∼0 | 21.66 ± 1.39ab (44.58 ± 5.53) | 5.29 ± 0.03b | 3.74 ± 0.31 | |
| 2.3 | 9.84 ± 0.95 | 38.53 ± 2.48b | 5.60 ± 0.03b | 0.31 ± 0.02b | 4.18 ± 0.33b | |
| With sTn reconstitution | ||||||
| Control | 1.9 | ∼0 | 51.83 ± 1.84 | 5.79 ± 0.01b | 3.20 ± 0.18b | |
| 2.3 | 10.23 ± 0.82 | 58.76 ± 3.14b | 5.93 ± 0.02b | 0.14 ± 0.02b | 3.00 ± 0.08 | |
| +ADP | 1.9 | ∼0 | 44.75 ± 5.61 (48.07 ± 4.49) | 5.94 ± 0.02bc | 3.38 ± 0.18 | |
| 2.3 | 8.16 ± 0.49 | 49.90 ± 4.76b | 6.01 ± 0.02bc | 0.07 ± 0.01bc | 3.52 ± 0.19c | |
| +Pi | 1.9 | ∼0 | 22.36 ± 1.19abc (57.17 ± 2.70) | 5.50 ± 0.04c | 2.39 ± 0.29bc | |
| 2.3 | 9.49 ± 0.13 | 44.96 ± 2.32b | 5.74 ± 0.04c | 0.24 ± 0.02c | 2.93 ± 0.30 | |
| SL | Passive force | Maximal force | pCa50 | ΔpCa50 | nH | |
| µm | mN/mm2 | mN/mm2 | ||||
| Without sTn reconstitution | ||||||
| Control | 1.9 | ∼0 | 57.25 ± 2.29 | 5.56 ± 0.01 | 3.86 ± 0.13 | |
| 2.3 | 9.40 ± 0.50 | 73.79 ± 3.72 | 5.80 ± 0.01 | 0.24 ± 0.01 | 3.17 ± 0.24 | |
| +ADP | 1.9 | ∼0 | 67.19 ± 1.39a (78.41 ± 2.97) | 5.82 ± 0.01b | 3.44 ± 0.10b | |
| 2.3 | 8.10 ± 0.67 | 75.61 ± 2.64 | 5.93 ± 0.01b | 0.10 ± 0.01b | 3.06 ± 0.16 | |
| +Pi | 1.9 | ∼0 | 21.66 ± 1.39ab (44.58 ± 5.53) | 5.29 ± 0.03b | 3.74 ± 0.31 | |
| 2.3 | 9.84 ± 0.95 | 38.53 ± 2.48b | 5.60 ± 0.03b | 0.31 ± 0.02b | 4.18 ± 0.33b | |
| With sTn reconstitution | ||||||
| Control | 1.9 | ∼0 | 51.83 ± 1.84 | 5.79 ± 0.01b | 3.20 ± 0.18b | |
| 2.3 | 10.23 ± 0.82 | 58.76 ± 3.14b | 5.93 ± 0.02b | 0.14 ± 0.02b | 3.00 ± 0.08 | |
| +ADP | 1.9 | ∼0 | 44.75 ± 5.61 (48.07 ± 4.49) | 5.94 ± 0.02bc | 3.38 ± 0.18 | |
| 2.3 | 8.16 ± 0.49 | 49.90 ± 4.76b | 6.01 ± 0.02bc | 0.07 ± 0.01bc | 3.52 ± 0.19c | |
| +Pi | 1.9 | ∼0 | 22.36 ± 1.19abc (57.17 ± 2.70) | 5.50 ± 0.04c | 2.39 ± 0.29bc | |
| 2.3 | 9.49 ± 0.13 | 44.96 ± 2.32b | 5.74 ± 0.04c | 0.24 ± 0.02c | 2.93 ± 0.30 | |
Data are for Figs. 2 and 3. Maximal force was obtained by activating muscle at pCa 4.5 before construction of the force–pCa curve at each SL (passive force was measured just before activation at pCa 4.5). Numbers in parentheses indicate maximal force values obtained before ADP or Pi application. Maximal force obtained before sTn reconstitution at SL 1.9 µm: 54.36 ± 2.80, 48.65 ± 3.66, and 61.35 ± 2.08 mN/mm2 for the control, ADP-, and Pi-treated group, respectively (P > 0.05 compared with the value after sTn reconstitution).
P < 0.05 compared with the prior value.
P < 0.05 compared with the corresponding values in control group without sTn reconstitution.
P < 0.05 compared with the corresponding values in control group with sTn reconstitution.