Sequences of primers used for qPCR and ORF subcloning
| Primers . | 5′–3′ sequences . |
|---|---|
| qPCR | |
| RPLPO forward | GTGATGTGCAGCTGATCAAGA |
| RPLPO reverse | GATGACCAGCCCAAAGGAGA |
| β2-microglobulin forward | TTCACCCCCACTGAAAAAGAT |
| β2-microglobulin reverse | GGCATCTTCAAACCTCCATGAT |
| meloe forward | GTCCTCCCCAGCACCAGAGT |
| meloe reverse | AGCCTGCCATCTGCAATCCT |
| ORF subcloning | |
| (285–404) forward | aaaGAATTCgccaccATGAGTGAAAATGCAGGAGG |
| (285–404) reverse | aaaCTCGAGTCACTGGCACAGTGCAG |
| (486–689) forward | aaaGAATTCgccaccATGTCCGTAGGGAGACGC |
| (486–689) reverse | aaaCTCGAGTCACCCTAGAGCTGCCAAG |
| (1,105–1,194) forward | aaaGAATTCgccaccATGAAATTTGAATTAATTTGCAGAAC |
| (1,105–1,194) reverse | aaaCTCGAGTTAATTCCTAAGGCATTCATTC |
| (1,230–1,370) forward | aaaGAATTCgccaccATGAGTTGTGTAGGTTATCC |
| (1,230–1,370) reverse | aaaCTCGAGTCACAGGGATGCCGGCC |
| Primers . | 5′–3′ sequences . |
|---|---|
| qPCR | |
| RPLPO forward | GTGATGTGCAGCTGATCAAGA |
| RPLPO reverse | GATGACCAGCCCAAAGGAGA |
| β2-microglobulin forward | TTCACCCCCACTGAAAAAGAT |
| β2-microglobulin reverse | GGCATCTTCAAACCTCCATGAT |
| meloe forward | GTCCTCCCCAGCACCAGAGT |
| meloe reverse | AGCCTGCCATCTGCAATCCT |
| ORF subcloning | |
| (285–404) forward | aaaGAATTCgccaccATGAGTGAAAATGCAGGAGG |
| (285–404) reverse | aaaCTCGAGTCACTGGCACAGTGCAG |
| (486–689) forward | aaaGAATTCgccaccATGTCCGTAGGGAGACGC |
| (486–689) reverse | aaaCTCGAGTCACCCTAGAGCTGCCAAG |
| (1,105–1,194) forward | aaaGAATTCgccaccATGAAATTTGAATTAATTTGCAGAAC |
| (1,105–1,194) reverse | aaaCTCGAGTTAATTCCTAAGGCATTCATTC |
| (1,230–1,370) forward | aaaGAATTCgccaccATGAGTTGTGTAGGTTATCC |
| (1,230–1,370) reverse | aaaCTCGAGTCACAGGGATGCCGGCC |
The italicized letters correspond to the enzyme restriction sites. The lowercased letters correspond to the Kozak sequence, which is described in the Materials and methods.