Prevention of Colitis Is Independent of IL-10 Production by CD45RBhigh CD4+ T Cells
| Phenotype of CD4+ T cells injected . | No or minimal colitis (0–1) . | Mild colitis (2) . | Severe colitis (3–5) . |
|---|---|---|---|
| 4 × 105 IL-10−/− CD45RBhigh | 3/25 (12.0%) | 5/25 | 17/25 (68.0%) |
| 4 × 105 IL-10−/− CD45RBhigh | |||
| + 2 × 105 CD45RBlow | 26/32 (81.2%) | 2/32 | 4/32 (12.5%) |
| Phenotype of CD4+ T cells injected . | No or minimal colitis (0–1) . | Mild colitis (2) . | Severe colitis (3–5) . |
|---|---|---|---|
| 4 × 105 IL-10−/− CD45RBhigh | 3/25 (12.0%) | 5/25 | 17/25 (68.0%) |
| 4 × 105 IL-10−/− CD45RBhigh | |||
| + 2 × 105 CD45RBlow | 26/32 (81.2%) | 2/32 | 4/32 (12.5%) |
RAG-2−/− mice were reconstituted with sorted CD4+ T cell subsets. 10–12 wk after reconstitution, mice were killed and colonic pathology was graded. Data are pooled from four to six independent experiments.