| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-HA mouse | Covance/BioLegend | MMS101R |
| Anti-HA rabbit | Cell Signaling Technologies | 3724 |
| Anti–β-Actin mouse | Sigma | A1978 |
| Anti-MAP2 chicken | Encor | CPCA MAP2 |
| Anti-MAP2 mouse | Sigma | M1406 |
| Anti–PSD-95 mouse | Synaptic Systems | 124011 |
| Anti-vGLUT1 guinea pig | Millipore | AB5905 |
| Anti-Homer1 rabbit | Synaptic Systems | 160003 |
| Anti-Homer1 rabbit | Millipore | ABN37 |
| Anti-gephyrin mouse | Synaptic Systems | 147111 |
| Goat anti-mouse Alexa Fluor 488 | Thermo Fisher Scientific | A11001 |
| Goat anti-mouse Alexa Fluor 546 | Thermo Fisher Scientific | A11003 |
| Goat anti-mouse Alexa Fluor 647 | Thermo Fisher Scientific | A21236 |
| Goat anti-rabbit Alexa Fluor 488 | Thermo Fisher Scientific | A11034 |
| Goat anti-rabbit Alexa Fluor 546 | Thermo Fisher Scientific | A11010 |
| Goat anti-rabbit Alexa Fluor 647 | Thermo Fisher Scientific | A21245 |
| Goat anti-chicken Alexa Fluor 647 | Thermo Fisher Scientific | A21449 |
| Goat anti–guinea pig Alexa Fluor 647 | Thermo Fisher Scientific | A21450 |
| Anti-mouse CF568 | Biotium | 20105-1mg |
| Donkey anti-mouse 800CW | Licor | 92632212 |
| Donkey anti-rabbit 800CW | Licor | 92632213 |
| Bacterial and virus strains | ||
| DH10β | Thermo Fisher Scientific | 18297010 |
| Lentiviral VSVG | Commonly available plasmid | N/A |
| Adeno-associated virus DJ capsid | Commonly available plasmid | N/A |
| Biological samples | ||
| Primary hippocampal cultures | CD1 mice from Charles River Laboratories | N/A |
| Hippocampal brain tissue | C57/Bl6 mice | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Streptavidin Alexa Fluor 488 | Thermo Fisher Scientific | S32354 |
| Streptavidin Alexa Fluor 555 | Invitrogen | S21381 |
| Alexa Fluor 546 phalloidin | Invitrogen | A22283 |
| Cytosine arabinofuranoside | Sigma | C6645 |
| QX-314 | Tocris | 1014 |
| Picrotoxin | Tocris | 1128 |
| Tetrodotoxin | Tocris | 1069 |
| D-AP5 | Tocris | 0106 |
| CNQX | Tocris | 1045 |
| Benzonase nuclease | Sigma | E1014 |
| PFA | Electron Microscopy Science | 15714 |
| 2,2,2-tribromoethanol | Sigma | T48402 |
| Biocytin | Sigma | B4261 |
| Glucose oxidase | Sigma | G2133 |
| Catalase | Sigma | C40 |
| DAPI | Roche | 10236276001 |
| Critical commercial assays | ||
| N/A | ||
| Deposited data | ||
| All raw numerical data within figures are submitted with manuscript | ||
| Experimental models: cell lines | ||
| HEK293T | ATCC | CRL-11268 |
| Experimental models: organisms/strains | ||
| CD-1 | Charles River | N/A |
| C57/BL6 | The Jackson Laboratory | 000664 |
| Oligonucleotides | ||
| Various cloning primers | IDT | N/A |
| Recombinant DNA | ||
| Pink Flamindo cAMP reporter | Addgene | 102356 |
| pAAV CAG FLEX TVA-mCherry | Addgene | 48332 |
| pAAV CAG FLEX RG | Addgene | 48333 |
| Lenti HA-PDE7b | This paper | N/A |
| Lenti Syn PDE7b-Hr1 | This paper | N/A |
| Lenti EF1a PDE7b-Hr1 | This paper | N/A |
| Lenti Syn PDE7b*-Hr1 | This paper | N/A |
| Lenti Syn ER-PDE7b | This paper | N/A |
| Lenti Syn mitoPDE7b | This paper | N/A |
| Lenti Syn NLS-PDE7b | This paper | N/A |
| Lenti EF1a PKI-Hr1 | This paper | N/A |
| Lenti Syn Hr1 Nanobody7-PDE7b | This paper | N/A |
| Lenti Syn Hr1 Nanobody25-PDE7b | This paper | N/A |
| Lenti Syn Hr1 Nanobody7-YFP | Dong et al., 2019; ELife | N/A |
| Lenti Syn Hr1 Nanobody25-YFP | Dong et al., 2019; ELife | N/A |
| Lenti Syn HA-Homer1 | This paper | N/A |
| Lenti Syn GFP p2a PDE7b-Hr1 | This paper | N/A |
| Lenti Syn GFP p2a PDE7b*-Hr1 | This paper | N/A |
| Lenti EF1a PKI-Hr1 p2a tdTomato | This paper | N/A |
| Lenti EF1a Hr1 Nanobody7-PDE7b p2a tdTomato | This paper | N/A |
| pAAV CAG PDE7b-Hr1 | This paper | N/A |
| pAAV CAG PDE7b*-Hr1 | This paper | N/A |
| pAAV CAG PDE7b | This paper | N/A |
| Lenti Syn GFP-Homer1 | This paper | N/A |
| Software | ||
| SnapGene | GSL Biotech | |
| pClamp10 | Molecular Devices | |
| Clampfit 10 | Molecular Devices | |
| NIS-Elements AR | Nikon | |
| ImageJ | National Institutes of Health | |
| Adobe Photoshop | Adobe | |
| Adobe Illustrator | Adobe | |
| GraphPad Prism 8.0 | GraphPad | |
| Neurolucida360 | MBF Bioscience | |
| Vutara SRX | Bruker Vutara | |
| NeuronStudio | CNIC | |
| Other | ||
| N/A | ||
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Anti-HA mouse | Covance/BioLegend | MMS101R |
| Anti-HA rabbit | Cell Signaling Technologies | 3724 |
| Anti–β-Actin mouse | Sigma | A1978 |
| Anti-MAP2 chicken | Encor | CPCA MAP2 |
| Anti-MAP2 mouse | Sigma | M1406 |
| Anti–PSD-95 mouse | Synaptic Systems | 124011 |
| Anti-vGLUT1 guinea pig | Millipore | AB5905 |
| Anti-Homer1 rabbit | Synaptic Systems | 160003 |
| Anti-Homer1 rabbit | Millipore | ABN37 |
| Anti-gephyrin mouse | Synaptic Systems | 147111 |
| Goat anti-mouse Alexa Fluor 488 | Thermo Fisher Scientific | A11001 |
| Goat anti-mouse Alexa Fluor 546 | Thermo Fisher Scientific | A11003 |
| Goat anti-mouse Alexa Fluor 647 | Thermo Fisher Scientific | A21236 |
| Goat anti-rabbit Alexa Fluor 488 | Thermo Fisher Scientific | A11034 |
| Goat anti-rabbit Alexa Fluor 546 | Thermo Fisher Scientific | A11010 |
| Goat anti-rabbit Alexa Fluor 647 | Thermo Fisher Scientific | A21245 |
| Goat anti-chicken Alexa Fluor 647 | Thermo Fisher Scientific | A21449 |
| Goat anti–guinea pig Alexa Fluor 647 | Thermo Fisher Scientific | A21450 |
| Anti-mouse CF568 | Biotium | 20105-1mg |
| Donkey anti-mouse 800CW | Licor | 92632212 |
| Donkey anti-rabbit 800CW | Licor | 92632213 |
| Bacterial and virus strains | ||
| DH10β | Thermo Fisher Scientific | 18297010 |
| Lentiviral VSVG | Commonly available plasmid | N/A |
| Adeno-associated virus DJ capsid | Commonly available plasmid | N/A |
| Biological samples | ||
| Primary hippocampal cultures | CD1 mice from Charles River Laboratories | N/A |
| Hippocampal brain tissue | C57/Bl6 mice | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Streptavidin Alexa Fluor 488 | Thermo Fisher Scientific | S32354 |
| Streptavidin Alexa Fluor 555 | Invitrogen | S21381 |
| Alexa Fluor 546 phalloidin | Invitrogen | A22283 |
| Cytosine arabinofuranoside | Sigma | C6645 |
| QX-314 | Tocris | 1014 |
| Picrotoxin | Tocris | 1128 |
| Tetrodotoxin | Tocris | 1069 |
| D-AP5 | Tocris | 0106 |
| CNQX | Tocris | 1045 |
| Benzonase nuclease | Sigma | E1014 |
| PFA | Electron Microscopy Science | 15714 |
| 2,2,2-tribromoethanol | Sigma | T48402 |
| Biocytin | Sigma | B4261 |
| Glucose oxidase | Sigma | G2133 |
| Catalase | Sigma | C40 |
| DAPI | Roche | 10236276001 |
| Critical commercial assays | ||
| N/A | ||
| Deposited data | ||
| All raw numerical data within figures are submitted with manuscript | ||
| Experimental models: cell lines | ||
| HEK293T | ATCC | CRL-11268 |
| Experimental models: organisms/strains | ||
| CD-1 | Charles River | N/A |
| C57/BL6 | The Jackson Laboratory | 000664 |
| Oligonucleotides | ||
| Various cloning primers | IDT | N/A |
| Recombinant DNA | ||
| Pink Flamindo cAMP reporter | Addgene | 102356 |
| pAAV CAG FLEX TVA-mCherry | Addgene | 48332 |
| pAAV CAG FLEX RG | Addgene | 48333 |
| Lenti HA-PDE7b | This paper | N/A |
| Lenti Syn PDE7b-Hr1 | This paper | N/A |
| Lenti EF1a PDE7b-Hr1 | This paper | N/A |
| Lenti Syn PDE7b*-Hr1 | This paper | N/A |
| Lenti Syn ER-PDE7b | This paper | N/A |
| Lenti Syn mitoPDE7b | This paper | N/A |
| Lenti Syn NLS-PDE7b | This paper | N/A |
| Lenti EF1a PKI-Hr1 | This paper | N/A |
| Lenti Syn Hr1 Nanobody7-PDE7b | This paper | N/A |
| Lenti Syn Hr1 Nanobody25-PDE7b | This paper | N/A |
| Lenti Syn Hr1 Nanobody7-YFP | Dong et al., 2019; ELife | N/A |
| Lenti Syn Hr1 Nanobody25-YFP | Dong et al., 2019; ELife | N/A |
| Lenti Syn HA-Homer1 | This paper | N/A |
| Lenti Syn GFP p2a PDE7b-Hr1 | This paper | N/A |
| Lenti Syn GFP p2a PDE7b*-Hr1 | This paper | N/A |
| Lenti EF1a PKI-Hr1 p2a tdTomato | This paper | N/A |
| Lenti EF1a Hr1 Nanobody7-PDE7b p2a tdTomato | This paper | N/A |
| pAAV CAG PDE7b-Hr1 | This paper | N/A |
| pAAV CAG PDE7b*-Hr1 | This paper | N/A |
| pAAV CAG PDE7b | This paper | N/A |
| Lenti Syn GFP-Homer1 | This paper | N/A |
| Software | ||
| SnapGene | GSL Biotech | |
| pClamp10 | Molecular Devices | |
| Clampfit 10 | Molecular Devices | |
| NIS-Elements AR | Nikon | |
| ImageJ | National Institutes of Health | |
| Adobe Photoshop | Adobe | |
| Adobe Illustrator | Adobe | |
| GraphPad Prism 8.0 | GraphPad | |
| Neurolucida360 | MBF Bioscience | |
| Vutara SRX | Bruker Vutara | |
| NeuronStudio | CNIC | |
| Other | ||
| N/A | ||
ATCC, American Type Culture Collection; N/A, not applicable; pAAV - plasmid adeno-associated virus; VSVG, vesicular stomatitis virus.