Table 2.
Primers used to synthesize ptf1α sgRNA
PrimerSequence (5′ to 3′)
Forward primer 1 TGT​AAT​ACG​ACT​CAC​TAT​AGG​TAG​TCG​GAT​ATC​ACA​GGA​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 2 TGT​AAT​ACG​ACT​CAC​TAT​AGG​GGC​TTA​CTG​AAG​AGG​CGG​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 3 TGT​AAT​ACG​ACT​CAC​TAT​AGG​CAG​TCG​GAC​ATG​CCA​ATT​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 4 TGT​AAT​ACG​ACT​CAC​TAT​AGG​AAG​TCG​TCT​GTG​TCC​AAG​GTT​TTA​GAG​CTA​GAA​AT 
Reverse primer AAA​AAA​AGC​ACC​GAC​TCG​GTG​CCA​C 
PrimerSequence (5′ to 3′)
Forward primer 1 TGT​AAT​ACG​ACT​CAC​TAT​AGG​TAG​TCG​GAT​ATC​ACA​GGA​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 2 TGT​AAT​ACG​ACT​CAC​TAT​AGG​GGC​TTA​CTG​AAG​AGG​CGG​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 3 TGT​AAT​ACG​ACT​CAC​TAT​AGG​CAG​TCG​GAC​ATG​CCA​ATT​GTT​TTA​GAG​CTA​GAA​AT 
Forward primer 4 TGT​AAT​ACG​ACT​CAC​TAT​AGG​AAG​TCG​TCT​GTG​TCC​AAG​GTT​TTA​GAG​CTA​GAA​AT 
Reverse primer AAA​AAA​AGC​ACC​GAC​TCG​GTG​CCA​C 
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