Table 1.
List of constructs used in this work, together with specifications about internal identification number (when it applies), vector, expression system, protein encoded, and reference study
Identification no.aVectorExpression systemEncodingReference/source
SMC1178 pBIG1b Sf9 (10xHis-TEVcs-)ATG5, ATG12 (synthetic gene) This study 
SMC1179 pBIG1a Sf9 ATG7, ATG10 (synthetic gene) This study 
SMC1099 pGBdest Sf9 ATG12, (10xHis-TEVcs-)ATG5, 10xHis-TEVcs-ATG16L1-TEVcs-StrepII, ATG7, ATG10 (synthetic genes) This study 
SMC1100 pGBdest Sf9 ATG12, (10xHis-TEVcs-ATG5), 10xHis-TEVcs-ATG16L1-GFP-TEVcs-StrepII, ATG7, ATG10 (synthetic genes) This study 
SMC911 pFast BacHT(B) Sf9 Mouse 6xHis-TEVcs-ATG7 Noor Gammoh laboratory 
SMC861 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-ATG3 This study 
SMC893 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-LC3B-Gly(Δ5C) This study 
SMC948 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-LC3B-Gly(Δ5C) Zaffagnini et al., 2018  
SMC1199 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-WIPI2d This study 
SMC1200 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-WIPI2d This study 
SMC872 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-Atg21 This study 
SMC929 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-Atg21 This study 
SMC1397 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-WIPI2d R108,125E This study 
SMC1392 pGEX4T-1 E. coli Rosetta pLysS GST-Thrcs-ATG4B This study 
Addgene 99329 pLEXm HEKGnTi GST-TEV-ATG14 (synthetic gene) Baskaran et al., 2014  
Addgene 99328 pCAG HEKGnTi OSF-TEV-BECN1 (synthetic gene) Baskaran et al., 2014  
Addgene 99327 pCAG HEKGnTi OSF-TEV-VPS34 (synthetic gene) Baskaran et al., 2014  
N/A pCAG HEKGnTi VPS15 (synthetic gene) Stjepanovic et al., 2017  
N/A pCAG HEKGnTi GST-TEV-GFP-ATG14 (synthetic gene) This study 
N/A pCAG HEKGnTi GST-TEV-mCherry-WIPI3 (synthetic gene) This study 
N/A pCAG HEKGnTi GST-TEV-WIPI3 (synthetic gene) This study 
N/A pCAG HEKGnTi WIPI2d-TEV-Strep This study 
N/A pCAG HEKGnTi WIPI2d-TEV-Strep (FRRG to AAAA) This study 
N/A pCAG HEKGnTi mCherry-WIPI2d-TEV-Strep This study 
N/A pCAG HEKGnTi mCherry-WIPI2d-TEV-Strep (FRRG to AAAA) This study 
N/A pLEXm HEKGnTi GST-TEV-mCherry-WIPI4 (synthetic gene) This study 
N/A pGST2 E. coli BL21 DE3 GST-TEV-mCherry-FYVE This study 
Identification no.aVectorExpression systemEncodingReference/source
SMC1178 pBIG1b Sf9 (10xHis-TEVcs-)ATG5, ATG12 (synthetic gene) This study 
SMC1179 pBIG1a Sf9 ATG7, ATG10 (synthetic gene) This study 
SMC1099 pGBdest Sf9 ATG12, (10xHis-TEVcs-)ATG5, 10xHis-TEVcs-ATG16L1-TEVcs-StrepII, ATG7, ATG10 (synthetic genes) This study 
SMC1100 pGBdest Sf9 ATG12, (10xHis-TEVcs-ATG5), 10xHis-TEVcs-ATG16L1-GFP-TEVcs-StrepII, ATG7, ATG10 (synthetic genes) This study 
SMC911 pFast BacHT(B) Sf9 Mouse 6xHis-TEVcs-ATG7 Noor Gammoh laboratory 
SMC861 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-ATG3 This study 
SMC893 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-LC3B-Gly(Δ5C) This study 
SMC948 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-LC3B-Gly(Δ5C) Zaffagnini et al., 2018  
SMC1199 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-WIPI2d This study 
SMC1200 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-WIPI2d This study 
SMC872 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-Atg21 This study 
SMC929 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-mCherry-Atg21 This study 
SMC1397 pET Duet-1 E. coli Rosetta pLysS 6xHis-TEVcs-WIPI2d R108,125E This study 
SMC1392 pGEX4T-1 E. coli Rosetta pLysS GST-Thrcs-ATG4B This study 
Addgene 99329 pLEXm HEKGnTi GST-TEV-ATG14 (synthetic gene) Baskaran et al., 2014  
Addgene 99328 pCAG HEKGnTi OSF-TEV-BECN1 (synthetic gene) Baskaran et al., 2014  
Addgene 99327 pCAG HEKGnTi OSF-TEV-VPS34 (synthetic gene) Baskaran et al., 2014  
N/A pCAG HEKGnTi VPS15 (synthetic gene) Stjepanovic et al., 2017  
N/A pCAG HEKGnTi GST-TEV-GFP-ATG14 (synthetic gene) This study 
N/A pCAG HEKGnTi GST-TEV-mCherry-WIPI3 (synthetic gene) This study 
N/A pCAG HEKGnTi GST-TEV-WIPI3 (synthetic gene) This study 
N/A pCAG HEKGnTi WIPI2d-TEV-Strep This study 
N/A pCAG HEKGnTi WIPI2d-TEV-Strep (FRRG to AAAA) This study 
N/A pCAG HEKGnTi mCherry-WIPI2d-TEV-Strep This study 
N/A pCAG HEKGnTi mCherry-WIPI2d-TEV-Strep (FRRG to AAAA) This study 
N/A pLEXm HEKGnTi GST-TEV-mCherry-WIPI4 (synthetic gene) This study 
N/A pGST2 E. coli BL21 DE3 GST-TEV-mCherry-FYVE This study 
a

N/A, not applicable.

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