Table 1.
Plasmids
NameDescriptionPromoterInsertTagsSelection
MS03 SHH Expression Plasmid CMV HsSHH None Bsd 
GP734 Flag-Ub CMV Ub Flag, Ub Bsd 
PD19 Flag-UbnoK (7 Lys mutated to Arg) CMV UbnoK Flag, UbnoK Bsd 
PD14 HA-Ub CMV Ub HA, Ub Puro 
PD13 HA-UbnoK (7 Lys mutated to Arg) CMV UbnoK HA, UbnoK Puro 
PD22 Smo-Flag CMV Smo Flag Bsd 
GP736 Smo-Flag-Ub CMV Smo Flag, Ub Bsd 
GP777 SmonoK-Flag CMV SmonoK Flag Bsd 
GP806 SmonoK-Flag-Ub CMV SmonoK Flag, Ub Bsd 
GP807 SmonoK-Flag-UbnoK CMV SmonoK Flag, UbnoK Bsd 
PD23 SmoM2-Flag CMV SmoW539L Flag Bsd 
GP735 SmoM2-Flag-Ub CMV SmoW539L Flag, Ub Bsd 
GP938 SmoPi-Flag CMV SmoR455A Flag Bsd 
GP940 SmoPi-Flag-Ub CMV SmoR455A Flag, Ub Bsd 
GP757 Sstr3-Flag CMV Sstr3 Flag Bsd 
GP758 Sstr3-Flag-Ub CMV Sstr3 Flag, Ub Bsd 
PD29 Drd1-Flag CMV Drd1 Flag Bsd 
PD30 Drd1-Flag-Ub CMV Drd1 Flag, Ub Bsd 
GP799 Smo-Flag GgCryD1 Smo Flag Bsd 
GP805 Like GP799 but with the 21 cytoplasmic lysines of Smo mutated to Arg 
GP802 Like GP799 but with the two lysines in loop two of Smo mutated to Arg  
GP803 Like GP799 but with the three lysines (KKK to RRR) in loop three of Smo mutated to Arg 
GP804 Like GP799 but with the 16 lysines in C-terminal tail of Smo mutated to Arg 
GP824 Like GP799 but with two lysines (KKK to RRK) in loop three of Smo mutated to Arg 
GP825 Like GP799 but with two lysines (KKK to RKR) in loop three of Smo mutated to Arg 
GP826 Like GP799 but with two lysines (KKK to KRR) in loop three of Smo mutated to Arg 
GP821 Like GP799 but with one lysine (KKK to RKK) in loop three of Smo mutated to Arg 
GP822 Like GP799 but with one lysine (KKK to KRK) in loop three of Smo mutated to Arg 
GP823 Like GP799 but with one lysine (KKK to KKR) in loop three of Smo mutated to Arg 
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