| Interaction . | Method . | Sources . |
|---|---|---|
| D1R-GluN1 | CoIP of rat brain tissue and hippocampal cultures | Lee et al., 2002; Fiorentini et al., 2003 |
| BRET in COS cells expressing the subunits | Fiorentini et al., 2003 | |
| CoIP using rat hippocampal extract | Pei et al., 2004 | |
| CoIP after disruption of interaction using peptide in mouse striatum neurons and slices | Cahill et al., 2014 | |
| SPT in rat hippocampal neurons | Ladepeche et al., 2013a | |
| D1R-GluN2A | CoIP of rat brain tissue, hippocampal cultures, and transfected heterologous cells | Lee et al., 2002; Fiorentini et al., 2003 |
| D2R-GluN2B | CoIP of GST-fused subunits using striatal extracts | Liu et al., 2006 |
| mGluR1-GluN1 | BRET in transfected HEK293 cells | Perroy et al., 2008 |
| mGluR1-GluN2A | CoIP with rat brain hippocampal lysates | Lai et al., 2019 |
| mGluR5-GluN1 | BRET in transfected HEK293 cells | Perroy et al., 2008 |
| SPT in rat hippocampal neurons | Aloisi et al., 2017 | |
| EphB2-GluN1 | CoIP of transfected HEK293 cells | Dalva et al., 2000 |
| CoIP of brain lysates; cultured neurons | Nolt et al., 2011; Hanamura et al., 2017 | |
| SPT in rat hippocampal neurons | Mikasova et al., 2012 | |
| ICC in synaptosomes from mouse brain (colocalization) and PLA | Washburn et al., 2020 | |
| µOR-GluN1 | CoIP assays with mice brain synaptosomes; BiFC approach in live transfected CHO cells | Rodríguez-Muñoz et al., 2012 |
| ApoE-GluN1 | CoIP of transfected COS7 cells and CoIP of mouse brain lysates | Hoe et al., 2006 |
| BKα R–GluN1 | CoIP of rodent brain | Zhang et al., 2018 |
| α7 nAchR-GluN2A | IP of rat hippocampal homogenate | Li et al., 2012 |
| Sig1R-GluN1 | CoIP, AFM, PLA | Balasuriya et al., 2013 |
| NL1–GluN1 | PLA in COS7 cells | Budreck et al., 2013 |
| H3R-GluN1, H3R-GluN2A | CoIP, PLA, BRET, BiFC in heterologous cells, and/or rodent brain tissue | Rodríguez-Ruiz et al., 2017 |
| IL1R-GluN1, IL1R-GluN2B | CoIP of rat hippocampi | Gardoni et al., 2011 |
| P2X-GluN1 | Electrophysiology experiments on exogenously expressed receptors in oocytes | Rodriguez et al., 2020 |
| TRPM4-GluN2A, TRPM4-GluN2B | CoIP of rodent brain tissue | Yan et al., 2020 |
| Interaction . | Method . | Sources . |
|---|---|---|
| D1R-GluN1 | CoIP of rat brain tissue and hippocampal cultures | Lee et al., 2002; Fiorentini et al., 2003 |
| BRET in COS cells expressing the subunits | Fiorentini et al., 2003 | |
| CoIP using rat hippocampal extract | Pei et al., 2004 | |
| CoIP after disruption of interaction using peptide in mouse striatum neurons and slices | Cahill et al., 2014 | |
| SPT in rat hippocampal neurons | Ladepeche et al., 2013a | |
| D1R-GluN2A | CoIP of rat brain tissue, hippocampal cultures, and transfected heterologous cells | Lee et al., 2002; Fiorentini et al., 2003 |
| D2R-GluN2B | CoIP of GST-fused subunits using striatal extracts | Liu et al., 2006 |
| mGluR1-GluN1 | BRET in transfected HEK293 cells | Perroy et al., 2008 |
| mGluR1-GluN2A | CoIP with rat brain hippocampal lysates | Lai et al., 2019 |
| mGluR5-GluN1 | BRET in transfected HEK293 cells | Perroy et al., 2008 |
| SPT in rat hippocampal neurons | Aloisi et al., 2017 | |
| EphB2-GluN1 | CoIP of transfected HEK293 cells | Dalva et al., 2000 |
| CoIP of brain lysates; cultured neurons | Nolt et al., 2011; Hanamura et al., 2017 | |
| SPT in rat hippocampal neurons | Mikasova et al., 2012 | |
| ICC in synaptosomes from mouse brain (colocalization) and PLA | Washburn et al., 2020 | |
| µOR-GluN1 | CoIP assays with mice brain synaptosomes; BiFC approach in live transfected CHO cells | Rodríguez-Muñoz et al., 2012 |
| ApoE-GluN1 | CoIP of transfected COS7 cells and CoIP of mouse brain lysates | Hoe et al., 2006 |
| BKα R–GluN1 | CoIP of rodent brain | Zhang et al., 2018 |
| α7 nAchR-GluN2A | IP of rat hippocampal homogenate | Li et al., 2012 |
| Sig1R-GluN1 | CoIP, AFM, PLA | Balasuriya et al., 2013 |
| NL1–GluN1 | PLA in COS7 cells | Budreck et al., 2013 |
| H3R-GluN1, H3R-GluN2A | CoIP, PLA, BRET, BiFC in heterologous cells, and/or rodent brain tissue | Rodríguez-Ruiz et al., 2017 |
| IL1R-GluN1, IL1R-GluN2B | CoIP of rat hippocampi | Gardoni et al., 2011 |
| P2X-GluN1 | Electrophysiology experiments on exogenously expressed receptors in oocytes | Rodriguez et al., 2020 |
| TRPM4-GluN2A, TRPM4-GluN2B | CoIP of rodent brain tissue | Yan et al., 2020 |
CoIP, coimmunoprecipitation; BRET, bioluminescence resonance energy transfer; COS cells, CV-1 (simian) in Origin, and carrying the SV40 cells; SPT, single particle tracking; HEK293 cells, human embryonic kidney 293 cells; ICC, immunocytochemestry; PLA, proximity ligase assay; BiFC, bimolecular fluorescence complementation; AFM, atomic force microscopy.