| Reagent or resource . | Source . |
|---|---|
| Plasmid DNA | |
| pMXs-puro retroviral vector | Cell Biolabs Inc. (RTV-012) |
| pBABE-puro retroviral vector | Addgene (#1764) |
| pcDNA3.1(+) | Invitrogen (V79020) |
| pBluescriptII SK(-) | Stratagene |
| pMXs-puro_YFP | This study |
| pMXs-puro_3FLAG-TEV | This study |
| pMXs-puro_3HA-TEV | This study |
| pMXs-puro_TEV-3FLAG | This study |
| pMXs-puro_TEV-3HA | This study |
| pBABE-puro_3FLAG-TEV | This study |
| pBABE-puro_3HA-TEV | This study |
| pBABE-puro_TEV-3FLAG | This study |
| pBABE-puro_TEV-3HA | This study |
| pcDNA3.1(+)_3FLAG-TEV | This study |
| pcDNA3.1(+)_3HA-TEV | This study |
| pcDNA3.1(+)_TEV-3FLAG | This study |
| pcDNA3.1(+)_TEV-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(K0)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(K0)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(K0)-3HA | This study |
| pMXs-puro_3FLAG-OPTN | This study |
| pMXs-puro_3FLAG-NDP52 | This study |
| pMXs-puro_3FLAG-p62 | This study |
| pMXs-puro_3FLAG-NBR1 | This study |
| pAsh-MCL | MBL (AM-8011M) |
| pHA-Ash-MCL | This study |
| pHA-Ash_6Ub | This study |
| pHA-Ash_6Ub(K0) | This study |
| phAG-MCL | MBL (AM-8011M) |
| phAG_OPTN | This study |
| phAG_OPTN (S177D) | This study |
| phAG_NDP52 | This study |
| phAG_p62 | This study |
| phAG_NBR1 | This study |
| pEYFP-C1 | Clontech (6006-1) |
| pUMVC (Gag-Pol) | Gift from Chunxin Wang |
| pCMV-VSV-G (VSV-G) | Gift from Chunxin Wang |
| pCHAC/EYFP-LC3B-IRES-MCS2 | Yamano et al., 2014 |
| pMXs-puro_3FLAG-LC3A | This study |
| pMXs-puro_3FLAG-LC3B | This study |
| pMXs-puro_3FLAG-LC3C | This study |
| pMXs-puro_3FLAG-GABARAP | This study |
| pMXs-puro_3FLAG-GABARAPL1 | This study |
| pMXs-puro_3FLAG-GABARAPL2 | This study |
| pWZL Neo Myr Flag TBK1 | Addgene (#20648) |
| pMXs-puro_3FLAG-TBK1 | This study |
| pMXS-IP GFP-Atg14 | Addgene (#38264) |
| pMXs-puro_3FLAG-ATG14 | This study |
| phAG_OPTN(S473E) | This study |
| phAG_OPTN(128-577, S473E) | This study |
| phAG_OPTN(150-577, S473E) | This study |
| phAG_OPTN(170-577, S473E) | This study |
| phAG_OPTN(190-577, S473E) | This study |
| phAG_OPTN(210-577, S473E) | This study |
| phAG_OPTN(301-577, S473E) | This study |
| phAG_OPTN(401-577, S473E) | This study |
| phAG_OPTN(1-530, S473E) | This study |
| phAG_OPTN(128-5A-132, S473E) | This study |
| phAG_OPTN(133-5A-137, S473E) | This study |
| phAG_OPTN(138-5A-142, S473E) | This study |
| phAG_OPTN(143-5A-147, S473E) | This study |
| phAG_OPTN(148-5A-152, S473E) | This study |
| phAG_OPTN(L143A, S473E) | This study |
| phAG_OPTN(L150A, S473E) | This study |
| phAG_OPTN(L157A, S473E) | This study |
| phAG_OPTN(L164A, S473E) | This study |
| phAG_OPTN(4LA, S473E) | This study |
| phAG_OPTN(M44Q/L54Q, S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(M44Q/L54Q, S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(4LA, S473E) | This study |
| pEF1-hspCas9-H1-gRNA TBK1-ex6-3 | This study |
| pUC57-Amp_TBK1-ex6 donor | This study |
| pUC57-Amp_TBK1-ex6 loxP-NeoR-donor | This study |
| pUC57-Amp_TBK1-ex6 loxP-HygroR-donor | This study |
| FRB-Fis1 | Lazarou et al., 2012 |
| PINK1Δ110-YFP-FKBP | Lazarou et al., 2012 |
| pOPTN(1-444)-2FKBP-HA | This study |
| pOPTN(1-444, 4LA)-2FKBP-HA | This study |
| pBMNz-YFP-Parkin | Lazarou et al., 2015 |
| pRetroQ-mt-Keima | Gift from Chunxin Wang |
| pBABE-puro_Untag-Parkin | This study |
| pMXs-puro_GFP-Parkin | Matsuda et al., 2010 |
| pMXs-puro_3FLAG-OPTN(4LA) | This study |
| pMXs-puro_3FLAG-OPTN(F178A) | This study |
| pMXs-puro_3FLAG-OPTN(4LA, F178A) | This study |
| p3xFLAG-CMV-10_CRABP-II | Okuhira et al., 2017 |
| pMXs-puro_3HA-TEV-CRABP-II | This study |
| pMXs-puro_CRABP-II-TEV-3HA | This study |
| pcDNA3.1-HA_HK1 | Okatsu et al., 2012 |
| pMXs-puro_HK1-CRABP-II-TEV-3HA | This study |
| pMXs-puro_TOMM20-CRABP-II-TEV-3HA | This study |
| Oligonucleotides | |
| siRNA PINK1 (GGGUCAGCACGUUCAGUUAdTdT) | Sigma-Aldrich |
| Reagent or resource . | Source . |
|---|---|
| Plasmid DNA | |
| pMXs-puro retroviral vector | Cell Biolabs Inc. (RTV-012) |
| pBABE-puro retroviral vector | Addgene (#1764) |
| pcDNA3.1(+) | Invitrogen (V79020) |
| pBluescriptII SK(-) | Stratagene |
| pMXs-puro_YFP | This study |
| pMXs-puro_3FLAG-TEV | This study |
| pMXs-puro_3HA-TEV | This study |
| pMXs-puro_TEV-3FLAG | This study |
| pMXs-puro_TEV-3HA | This study |
| pBABE-puro_3FLAG-TEV | This study |
| pBABE-puro_3HA-TEV | This study |
| pBABE-puro_TEV-3FLAG | This study |
| pBABE-puro_TEV-3HA | This study |
| pcDNA3.1(+)_3FLAG-TEV | This study |
| pcDNA3.1(+)_3HA-TEV | This study |
| pcDNA3.1(+)_TEV-3FLAG | This study |
| pcDNA3.1(+)_TEV-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-2Ub(K0)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-4Ub(K0)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(WT)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(K48R)-3HA | This study |
| pcDNA3.1(+)_T20(1-49)-YFP-6Ub(K0)-3HA | This study |
| pMXs-puro_3FLAG-OPTN | This study |
| pMXs-puro_3FLAG-NDP52 | This study |
| pMXs-puro_3FLAG-p62 | This study |
| pMXs-puro_3FLAG-NBR1 | This study |
| pAsh-MCL | MBL (AM-8011M) |
| pHA-Ash-MCL | This study |
| pHA-Ash_6Ub | This study |
| pHA-Ash_6Ub(K0) | This study |
| phAG-MCL | MBL (AM-8011M) |
| phAG_OPTN | This study |
| phAG_OPTN (S177D) | This study |
| phAG_NDP52 | This study |
| phAG_p62 | This study |
| phAG_NBR1 | This study |
| pEYFP-C1 | Clontech (6006-1) |
| pUMVC (Gag-Pol) | Gift from Chunxin Wang |
| pCMV-VSV-G (VSV-G) | Gift from Chunxin Wang |
| pCHAC/EYFP-LC3B-IRES-MCS2 | Yamano et al., 2014 |
| pMXs-puro_3FLAG-LC3A | This study |
| pMXs-puro_3FLAG-LC3B | This study |
| pMXs-puro_3FLAG-LC3C | This study |
| pMXs-puro_3FLAG-GABARAP | This study |
| pMXs-puro_3FLAG-GABARAPL1 | This study |
| pMXs-puro_3FLAG-GABARAPL2 | This study |
| pWZL Neo Myr Flag TBK1 | Addgene (#20648) |
| pMXs-puro_3FLAG-TBK1 | This study |
| pMXS-IP GFP-Atg14 | Addgene (#38264) |
| pMXs-puro_3FLAG-ATG14 | This study |
| phAG_OPTN(S473E) | This study |
| phAG_OPTN(128-577, S473E) | This study |
| phAG_OPTN(150-577, S473E) | This study |
| phAG_OPTN(170-577, S473E) | This study |
| phAG_OPTN(190-577, S473E) | This study |
| phAG_OPTN(210-577, S473E) | This study |
| phAG_OPTN(301-577, S473E) | This study |
| phAG_OPTN(401-577, S473E) | This study |
| phAG_OPTN(1-530, S473E) | This study |
| phAG_OPTN(128-5A-132, S473E) | This study |
| phAG_OPTN(133-5A-137, S473E) | This study |
| phAG_OPTN(138-5A-142, S473E) | This study |
| phAG_OPTN(143-5A-147, S473E) | This study |
| phAG_OPTN(148-5A-152, S473E) | This study |
| phAG_OPTN(L143A, S473E) | This study |
| phAG_OPTN(L150A, S473E) | This study |
| phAG_OPTN(L157A, S473E) | This study |
| phAG_OPTN(L164A, S473E) | This study |
| phAG_OPTN(4LA, S473E) | This study |
| phAG_OPTN(M44Q/L54Q, S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(M44Q/L54Q, S473E) | This study |
| pcDNA3.1(+)_3FLAG-hAG-OPTN(4LA, S473E) | This study |
| pEF1-hspCas9-H1-gRNA TBK1-ex6-3 | This study |
| pUC57-Amp_TBK1-ex6 donor | This study |
| pUC57-Amp_TBK1-ex6 loxP-NeoR-donor | This study |
| pUC57-Amp_TBK1-ex6 loxP-HygroR-donor | This study |
| FRB-Fis1 | Lazarou et al., 2012 |
| PINK1Δ110-YFP-FKBP | Lazarou et al., 2012 |
| pOPTN(1-444)-2FKBP-HA | This study |
| pOPTN(1-444, 4LA)-2FKBP-HA | This study |
| pBMNz-YFP-Parkin | Lazarou et al., 2015 |
| pRetroQ-mt-Keima | Gift from Chunxin Wang |
| pBABE-puro_Untag-Parkin | This study |
| pMXs-puro_GFP-Parkin | Matsuda et al., 2010 |
| pMXs-puro_3FLAG-OPTN(4LA) | This study |
| pMXs-puro_3FLAG-OPTN(F178A) | This study |
| pMXs-puro_3FLAG-OPTN(4LA, F178A) | This study |
| p3xFLAG-CMV-10_CRABP-II | Okuhira et al., 2017 |
| pMXs-puro_3HA-TEV-CRABP-II | This study |
| pMXs-puro_CRABP-II-TEV-3HA | This study |
| pcDNA3.1-HA_HK1 | Okatsu et al., 2012 |
| pMXs-puro_HK1-CRABP-II-TEV-3HA | This study |
| pMXs-puro_TOMM20-CRABP-II-TEV-3HA | This study |
| Oligonucleotides | |
| siRNA PINK1 (GGGUCAGCACGUUCAGUUAdTdT) | Sigma-Aldrich |