| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Reagents | ||
| Antimycin A | Sigma-Aldrich | Catalog number A8674 |
| Oligomycin | Calbiochem | Catalog number 495455-10MGCN |
| Valinomycin | Sigma-Aldrich | Catalog number V0627 |
| A/C heterodimerizer | Clontech | Catalog number 635057 |
| Q-VD-OPH | SM Biochemicals | Catalog number SMPH001 |
| Bafilomycin A1 | Calbiochem | Catalog number 196000 |
| SNIPER(CRABP)-11 | This study | N/A |
| DMEM | Sigma-Aldrich | Catalog number D5796 |
| McCoy’s 5A | Gibco | Catalog number 16600-082 |
| FBS | Biowest | Catalog number S1820 |
| MEM nonessential amino acids solution | Gibco | Catalog number 11140-050 |
| Sodium pyruvate | Gibco | Catalog number 11360-070 |
| Penicillin–Streptomycin–Glutamine | Gibco | Catalog number 10378-016 |
| GlutaMAX supplement | Gibco | Catalog number 35050-061 |
| Hepes | Gibco | Catalog number 15630-080 |
| Opti-MEM | Gibco | Catalog number 31985-070 |
| Trypsin-EDTA (0.25%) | Gibco | Catalog number 25200-056 |
| Puromycin | Sigma-Aldrich | Catalog number P8833 |
| G418 | Sigma-Aldrich | Catalog number G8168-10ML |
| Hygromycin B | Invitrogen | Catalog number 10687010 |
| EF1-hspCas9-H1-gRNA linearized SmartNuclease vector | System Biosciences | Catalog number CAS900A-1 |
| FuGENE6 | Promega | Catalog number E269A |
| Lipofectamine LTX | Invitrogen | Catalog number 15338100 |
| Lipofectamine RNAiMAX | Invitrogen | Catalog number 13778150 |
| Polybrene | Sigma-Aldrich | Catalog number H9268 |
| Dithiothreitol | Roche | Catalog number 10708984001 |
| CHAPS | Sigma-Aldrich | Catalog number C3023 |
| NP-40 | Nacalai Tesque | Catalog number 25223-75 |
| cOmplete, EDTA-free protease inhibitor cocktail | Roche | Catalog number 11873580001 |
| PhosSTOP, phosphatase inhibitor cocktail | Roche | Catalog number 04906845001 |
| MLN-7243 (E1 inhibitor) | Active Biochem | Catalog number A-1384 |
| BX-795 (TBK1 inhibitor) | Abcam | Catalog number ab142016 |
| NEM | Wako Pure Chemical | Catalog number 058-02061 |
| DDDDK-tagged Protein PURIFICATION GEL | MBL | Catalog number 3329 |
| DSP | Thermo Fisher Scientific | Catalog number 22585 |
| 4% PFA solution | Wako Pure Chemical | Catalog number 163-20145 |
| Gelatin | Sigma-Aldrich | Catalog number G9391 |
| Triton X-100 | MP Biomedicals | Catalog number 807426 |
| Digitonin | Wako Pure Chemical | Catalog number 043-21371 |
| Tween 20 | Nacalai Tesque | Catalog number 35624-15 |
| NuPAGE 4–12% Bis-Tris gel | Invitrogen | Catalog number NP0323BOX |
| NuPAGE MOPS SDS running buffer | Invitrogen | Catalog number NP0001 |
| NuPAGE MOPS SDS running buffer | Invitrogen | Catalog number NP0002 |
| Difco skim milk | BD Biosciences | Catalog number 232100 |
| BSA (fatty acid free) | Sigma-Aldrich | Catalog number A8806 |
| Phusion high-fidelity DNA polymerase | Thermo Fisher Scientific | Catalog number F-530L |
| DNA ligation kit | TaKaRa | Catalog number 6023 |
| Western Lightning Plus-ECL | PerkinElmer | Catalog number NEL105001EA |
| ECL Prime Western blotting detection reagents | GE Healthcare | Catalog number RPN2232 |
| Cell lines | ||
| HeLa (corresponds to WT for PINK1 KO HeLa) | Okatsu et al., 2015 | |
| PINK1 KO HeLa | Okatsu et al., 2015 | |
| HeLa (corresponds to WT for FIP200, ATG5, ATG9A, Penta KO HeLa) | ATCC | CCL-2,2 |
| FIP200 KO HeLa | Vargas et al., 2019 | |
| ATG5 KO HeLa | Nezich et al., 2015 | |
| ATG9A KO HeLa | Nezich et al., 2015 | |
| Penta (OPTN/NDP52/TAX1BP1/p62/NBR1) KO HeLa | Lazarou et al., 2015 | |
| HEK293T | ATCC | CRL-3216 |
| HT1080 | Itoh et al., 2012 | |
| HCT116 | Yamano et al., 2018 | |
| TBK1−/− HCT116 | This study | |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Reagents | ||
| Antimycin A | Sigma-Aldrich | Catalog number A8674 |
| Oligomycin | Calbiochem | Catalog number 495455-10MGCN |
| Valinomycin | Sigma-Aldrich | Catalog number V0627 |
| A/C heterodimerizer | Clontech | Catalog number 635057 |
| Q-VD-OPH | SM Biochemicals | Catalog number SMPH001 |
| Bafilomycin A1 | Calbiochem | Catalog number 196000 |
| SNIPER(CRABP)-11 | This study | N/A |
| DMEM | Sigma-Aldrich | Catalog number D5796 |
| McCoy’s 5A | Gibco | Catalog number 16600-082 |
| FBS | Biowest | Catalog number S1820 |
| MEM nonessential amino acids solution | Gibco | Catalog number 11140-050 |
| Sodium pyruvate | Gibco | Catalog number 11360-070 |
| Penicillin–Streptomycin–Glutamine | Gibco | Catalog number 10378-016 |
| GlutaMAX supplement | Gibco | Catalog number 35050-061 |
| Hepes | Gibco | Catalog number 15630-080 |
| Opti-MEM | Gibco | Catalog number 31985-070 |
| Trypsin-EDTA (0.25%) | Gibco | Catalog number 25200-056 |
| Puromycin | Sigma-Aldrich | Catalog number P8833 |
| G418 | Sigma-Aldrich | Catalog number G8168-10ML |
| Hygromycin B | Invitrogen | Catalog number 10687010 |
| EF1-hspCas9-H1-gRNA linearized SmartNuclease vector | System Biosciences | Catalog number CAS900A-1 |
| FuGENE6 | Promega | Catalog number E269A |
| Lipofectamine LTX | Invitrogen | Catalog number 15338100 |
| Lipofectamine RNAiMAX | Invitrogen | Catalog number 13778150 |
| Polybrene | Sigma-Aldrich | Catalog number H9268 |
| Dithiothreitol | Roche | Catalog number 10708984001 |
| CHAPS | Sigma-Aldrich | Catalog number C3023 |
| NP-40 | Nacalai Tesque | Catalog number 25223-75 |
| cOmplete, EDTA-free protease inhibitor cocktail | Roche | Catalog number 11873580001 |
| PhosSTOP, phosphatase inhibitor cocktail | Roche | Catalog number 04906845001 |
| MLN-7243 (E1 inhibitor) | Active Biochem | Catalog number A-1384 |
| BX-795 (TBK1 inhibitor) | Abcam | Catalog number ab142016 |
| NEM | Wako Pure Chemical | Catalog number 058-02061 |
| DDDDK-tagged Protein PURIFICATION GEL | MBL | Catalog number 3329 |
| DSP | Thermo Fisher Scientific | Catalog number 22585 |
| 4% PFA solution | Wako Pure Chemical | Catalog number 163-20145 |
| Gelatin | Sigma-Aldrich | Catalog number G9391 |
| Triton X-100 | MP Biomedicals | Catalog number 807426 |
| Digitonin | Wako Pure Chemical | Catalog number 043-21371 |
| Tween 20 | Nacalai Tesque | Catalog number 35624-15 |
| NuPAGE 4–12% Bis-Tris gel | Invitrogen | Catalog number NP0323BOX |
| NuPAGE MOPS SDS running buffer | Invitrogen | Catalog number NP0001 |
| NuPAGE MOPS SDS running buffer | Invitrogen | Catalog number NP0002 |
| Difco skim milk | BD Biosciences | Catalog number 232100 |
| BSA (fatty acid free) | Sigma-Aldrich | Catalog number A8806 |
| Phusion high-fidelity DNA polymerase | Thermo Fisher Scientific | Catalog number F-530L |
| DNA ligation kit | TaKaRa | Catalog number 6023 |
| Western Lightning Plus-ECL | PerkinElmer | Catalog number NEL105001EA |
| ECL Prime Western blotting detection reagents | GE Healthcare | Catalog number RPN2232 |
| Cell lines | ||
| HeLa (corresponds to WT for PINK1 KO HeLa) | Okatsu et al., 2015 | |
| PINK1 KO HeLa | Okatsu et al., 2015 | |
| HeLa (corresponds to WT for FIP200, ATG5, ATG9A, Penta KO HeLa) | ATCC | CCL-2,2 |
| FIP200 KO HeLa | Vargas et al., 2019 | |
| ATG5 KO HeLa | Nezich et al., 2015 | |
| ATG9A KO HeLa | Nezich et al., 2015 | |
| Penta (OPTN/NDP52/TAX1BP1/p62/NBR1) KO HeLa | Lazarou et al., 2015 | |
| HEK293T | ATCC | CRL-3216 |
| HT1080 | Itoh et al., 2012 | |
| HCT116 | Yamano et al., 2018 | |
| TBK1−/− HCT116 | This study | |