| Crosses . | Experiment . |
|---|---|
| Rab2-Gal4-KO (#53) × Bsc260 (Rab2 deficiency) | Rab2 knockout (null mutant) (rab2−/−) |
| w1118 | As wild-type control for mutant |
| Rab2-Gal4-KO (#53) × Bsc260; UAS-Rab2-YFP (wild-type) | Rab2 rescue (description, see below under "Rescue experiment") |
| Ok6-Gal4, UAS-dicer2 × UAS-Rab2-RNAi105358; UAS-Rab2-RNAi34767 Ok6-Gal4, UAS-dicer2 × w1118 | Rab2 knockdown (RNAi) and control |
| Genomic Rab2-GFP | Rab2 localization (Figs. 3, 4, and S5) |
| Ok6-Gal4 × UAS-Rab2-YFP (wild-type) | In vivo (Fig. 3) Rab2 localization (Fig. S5) |
| Ok6-Gal4 × UAS-Rab2Q65L- YFP | In vivo (Fig. S4) Rab2 localization (Fig. S6) |
| Ok6-Gal4, UAS-BRP-D3-Straw × UAS-Rab2-YFP (wild-type) | In vivo (Fig. 3) |
| Ok6-Gal4, UAS-Rab2-YFP (wild-type) × UAS-Spinster-RFP | In vivo (Fig. 3) |
| Ok6-Gal4, UAS-BRP-D3-Straw × UAS-Rab2Q65L-YFP | In vivo (Fig. S5) |
| Ok6-Gal4, UAS-Rab2Q65L-YFP × UAS-Spinster-RFP | In vivo (Fig. S5) |
| UAS-dicer2 × UAS-Sec23-RNAi UAS-dicer2 × UAS-Arf79F-RNAi UAS-dicer2 × w1118 | Golgi disruption and control (Figs. 4 and S6) |
| rab2−/−, tub-GFP-LAMP1 | Fig. 7 |
| PBac(RB)Gie e00336/PBac(RB)Gie e00336 | arl8−/− mutant |
| arl8−/−, tub-GFP-LAMP1 | Fig. 7 |
| Bsc260; UAS-Arl8-GFP × Ok6-Gal4, Rab2-Gal4-KO (#53) | Fig. S8 |
| Rab2-Gal4-KO (#53); PBac(RB)Gie e00336 × Bsc260; PBac(RB)Gie e00336 | rab2−/−; arl8−/− double mutant |
| Crosses . | Experiment . |
|---|---|
| Rab2-Gal4-KO (#53) × Bsc260 (Rab2 deficiency) | Rab2 knockout (null mutant) (rab2−/−) |
| w1118 | As wild-type control for mutant |
| Rab2-Gal4-KO (#53) × Bsc260; UAS-Rab2-YFP (wild-type) | Rab2 rescue (description, see below under "Rescue experiment") |
| Ok6-Gal4, UAS-dicer2 × UAS-Rab2-RNAi105358; UAS-Rab2-RNAi34767 Ok6-Gal4, UAS-dicer2 × w1118 | Rab2 knockdown (RNAi) and control |
| Genomic Rab2-GFP | Rab2 localization (Figs. 3, 4, and S5) |
| Ok6-Gal4 × UAS-Rab2-YFP (wild-type) | In vivo (Fig. 3) Rab2 localization (Fig. S5) |
| Ok6-Gal4 × UAS-Rab2Q65L- YFP | In vivo (Fig. S4) Rab2 localization (Fig. S6) |
| Ok6-Gal4, UAS-BRP-D3-Straw × UAS-Rab2-YFP (wild-type) | In vivo (Fig. 3) |
| Ok6-Gal4, UAS-Rab2-YFP (wild-type) × UAS-Spinster-RFP | In vivo (Fig. 3) |
| Ok6-Gal4, UAS-BRP-D3-Straw × UAS-Rab2Q65L-YFP | In vivo (Fig. S5) |
| Ok6-Gal4, UAS-Rab2Q65L-YFP × UAS-Spinster-RFP | In vivo (Fig. S5) |
| UAS-dicer2 × UAS-Sec23-RNAi UAS-dicer2 × UAS-Arf79F-RNAi UAS-dicer2 × w1118 | Golgi disruption and control (Figs. 4 and S6) |
| rab2−/−, tub-GFP-LAMP1 | Fig. 7 |
| PBac(RB)Gie e00336/PBac(RB)Gie e00336 | arl8−/− mutant |
| arl8−/−, tub-GFP-LAMP1 | Fig. 7 |
| Bsc260; UAS-Arl8-GFP × Ok6-Gal4, Rab2-Gal4-KO (#53) | Fig. S8 |
| Rab2-Gal4-KO (#53); PBac(RB)Gie e00336 × Bsc260; PBac(RB)Gie e00336 | rab2−/−; arl8−/− double mutant |