Table 2.
Target gRNA sequences and PCR primers used for KO cell generation in this study
GeneExonTarget sequenceForward primerReverse primer
PEX5 6  5′-AGG​GAA​CAG​CCA​CCG​ATC​GC-3′ 5′-GTG​TGG​CTA​AGA​GGG​TCA​GT-3′ 5′-AAG​TTG​GTT​CGA​GAA​CGT​GGA​T-3′ 
PEX14 3  5′-TGT​AGA​AAC​TTC​ACT​GCC​G-3′ 5′-CTG​CCG​GGG​AGT​ATG​AAG​AA-3′ 5′-GTC​ATC​TTG​CCG​CTC​CAT​TG-3′ 
PEX19 4  5′-TGC​CAC​TGA​CCT​TCA​GGT​G-3′ 5′-TGA​AAG​GTC​ACA​GTG​TTA​TTG​T-3′ 5′-ACA​GCA​CAT​CCT​TGG​AGA​GT-3′ 
VPS13A 3 5′ 5′-CTT​CTT​TTT​CTT​AAA​ATA​A-3′ 5′-TGG​GGA​GAA​CGT​TTT​GAA​AG-3′ 5′-TTC​AAA​TCG​GCC​CCT​AGT​CA-3′ 
3′ 5′-CCT​TCC​TGG​ACA​TGC​AGC​C-3′ 
VPS13B 8 5′ 5′-TAT​CTT​TCT​CCT​TTA​GTA​G-3′ 5′-GGG​TGC​TGA​TCA​GGA​AAT​GAA​G-3′ 5′-ACG​AAG​GAG​AGC​ACA​GAA​AGG​A-3′ 
3′ 5′-CCA​CTT​GAA​ATC​TTT​GAA​T-3′ 
VPS13C 8 5′ 5′-TTG​AGT​AGT​ACC​TTA​GCT​A-3′ 5′-GCT​TCC​ACT​GTG​AAG​GAC​GTA-3′ 5′-TGA​GAC​TAC​AGT​CAA​GTG​ACC​A-3′ 
3′ 5′-CTT​GTG​TTA​GCC​TCG​TTT​T-3′ 
VPS13D 13  5′-CAT​ACA​CGA​GGC​ATC​TGC​AG-3′ 5′-GCT​CCT​CCT​GGC​GGA​ATT​TA-3′ 5′-GAC​ATT​GGC​CCA​CAG​AGA​GA-3′ 
VPS13D 19  5′-CAT​ACA​CGA​GGC​ATC​TGC​AG-3′ 5′-GCT​CCT​CCT​GGC​GGA​ATT​TA-3′ 5′-ACG​TGC​TAC​CCA​TTG​AGA​CA-3′ 
VPS13D 21  5′-AAG​AAC​CTG​ATG​GTG​TCT​CG-3′ 5′-CTC​CCC​TTG​CCC​TGA​TTC​TC-3′ 5′-AAC​CCA​GGT​TTG​CAG​TGT​GA-3′ 
FIP200 4–5 5' 5′-ACT​ATG​TAA​AAA​CAC​CTT​AG-3′ 5′-AGA​CCT​GAT​AAC​CAG​TTT​GAG​CAT-3′ 5′-TGT​CAA​ACT​TTT​TGC​ATA​CTT​CCT-3′ 
3' 5'-GTA​GTT​TTA​GGA​ATA​GCA​GG-3' 
GeneExonTarget sequenceForward primerReverse primer
PEX5 6  5′-AGG​GAA​CAG​CCA​CCG​ATC​GC-3′ 5′-GTG​TGG​CTA​AGA​GGG​TCA​GT-3′ 5′-AAG​TTG​GTT​CGA​GAA​CGT​GGA​T-3′ 
PEX14 3  5′-TGT​AGA​AAC​TTC​ACT​GCC​G-3′ 5′-CTG​CCG​GGG​AGT​ATG​AAG​AA-3′ 5′-GTC​ATC​TTG​CCG​CTC​CAT​TG-3′ 
PEX19 4  5′-TGC​CAC​TGA​CCT​TCA​GGT​G-3′ 5′-TGA​AAG​GTC​ACA​GTG​TTA​TTG​T-3′ 5′-ACA​GCA​CAT​CCT​TGG​AGA​GT-3′ 
VPS13A 3 5′ 5′-CTT​CTT​TTT​CTT​AAA​ATA​A-3′ 5′-TGG​GGA​GAA​CGT​TTT​GAA​AG-3′ 5′-TTC​AAA​TCG​GCC​CCT​AGT​CA-3′ 
3′ 5′-CCT​TCC​TGG​ACA​TGC​AGC​C-3′ 
VPS13B 8 5′ 5′-TAT​CTT​TCT​CCT​TTA​GTA​G-3′ 5′-GGG​TGC​TGA​TCA​GGA​AAT​GAA​G-3′ 5′-ACG​AAG​GAG​AGC​ACA​GAA​AGG​A-3′ 
3′ 5′-CCA​CTT​GAA​ATC​TTT​GAA​T-3′ 
VPS13C 8 5′ 5′-TTG​AGT​AGT​ACC​TTA​GCT​A-3′ 5′-GCT​TCC​ACT​GTG​AAG​GAC​GTA-3′ 5′-TGA​GAC​TAC​AGT​CAA​GTG​ACC​A-3′ 
3′ 5′-CTT​GTG​TTA​GCC​TCG​TTT​T-3′ 
VPS13D 13  5′-CAT​ACA​CGA​GGC​ATC​TGC​AG-3′ 5′-GCT​CCT​CCT​GGC​GGA​ATT​TA-3′ 5′-GAC​ATT​GGC​CCA​CAG​AGA​GA-3′ 
VPS13D 19  5′-CAT​ACA​CGA​GGC​ATC​TGC​AG-3′ 5′-GCT​CCT​CCT​GGC​GGA​ATT​TA-3′ 5′-ACG​TGC​TAC​CCA​TTG​AGA​CA-3′ 
VPS13D 21  5′-AAG​AAC​CTG​ATG​GTG​TCT​CG-3′ 5′-CTC​CCC​TTG​CCC​TGA​TTC​TC-3′ 5′-AAC​CCA​GGT​TTG​CAG​TGT​GA-3′ 
FIP200 4–5 5' 5′-ACT​ATG​TAA​AAA​CAC​CTT​AG-3′ 5′-AGA​CCT​GAT​AAC​CAG​TTT​GAG​CAT-3′ 5′-TGT​CAA​ACT​TTT​TGC​ATA​CTT​CCT-3′ 
3' 5'-GTA​GTT​TTA​GGA​ATA​GCA​GG-3' 
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