| Plasmid cloning . | Primer . | Sequence . |
|---|---|---|
| GFP-Ub-VPS13D | EGFP-Ub forward | 5′-TCTAGATAACTGATCATAATCAGCCATAC-3′ |
| GFP-Ub-VPS13D | EGFPC1 forward | 5′-GATCACTCTCGGCATGGAC-3′ |
| GFP-Ub-VPS13D | EGFPC1 reverse | 5′-CATTTTATGTTTCAGGTTCAGGG-3′ |
| GFP-Ub-VPS13D | Ub-Vps13D forward | 5′-TACTCCGTCTCAGAGGTGGGATGTTGGAAGGCCTTGTAGC-3′ |
| GFP-Ub-VPS13D | Ub-Vps13D reverse | 5′-ATTATGATCAGTTATCTAGATCAGGAGTCCAGCTCCAGCTG-3′ |
| GFP-Ub-VPS13DΔC | Ub-Vps13D-dC reverse | 5′-ATTATGATCAGTTATCTAGATCAAATTGGTGTTCCACCTTG-3′ |
| GFP-Ub-VPS13DΔN | Ub-Vps13D-dN forward | 5′-TACTCCGTCTCAGAGGTGGGATGGAGGAGAAATGGAAGAATGACC-3′ |
| GFP-Ub-VPS13DΔN | Ub-Vps13D-dN reverse | 5′-ATTATGATCAGTTATCTAGATCAGGAGTCCAGCTCCAGCTG-3′ |
| GFP-Ub-VPS13DmutG1200D | Vps13D-G1200D forward | 5′-AGTATAGGTGACACCAAAGTTAATGTCTCAATG-3′ |
| GFP-Ub-VPS13DmutG1200D | Vps13D-G1200D reverse | 5′-ACTTTGGTGTCACCTATACTTGCAGTTG-3′ |
| GFP-Ub-VPS13DmutG4149S | Vps13D-G4149S forward | 5′-CCTTGTAGCCAGCATCCATGGCCTGGCTC-3′ |
| GFP-Ub-VPS13DmutG4149S | Vps13D-G4149S reverse | 5′-CATGGATGCTGGCTACAAGGTGTTCACC-3′ |
| GFP-Ub-VPS13DmutL2900S | Vps13D-L2900S forward | 5′-GGGTGCACTTCGTGGTTTGCCACCCTGAC-3′ |
| GFP-Ub-VPS13DmutL2900S | Vps13D-L2900S reverse | 5′-GCAAACCACGAAGTGCACCCCGTGTGGT-3′ |
| GFP-Ub-VPS13DmutN34107I | Vps13D-N4107I forward | 5′-GGAGTATCAATCTCTGCTGCCAAGTTTGC-3′ |
| GFP-Ub-VPS13DmutN34107I | Vps13D-N4107I reverse | 5′-TTGGCAGCAGAGATTGATACTCCGTGTGTAACATTTC-3′ |
| GFP-Ub-VPS13DmutN3521S | Vps13D-N3521S forward | 5′-CGAATTGACAGCTTTTCTAAGGTCCCGGTTG-3′ |
| GFP-Ub-VPS13DmutN3521S | Vps13D-N3521S reverse | 5′-TTAGAAAAGCTGTCAATTCGGAAAGGAG-3′ |
| GFP-Ub-VPS13DmutR4228Q | Vps13D reverse4228Q forward | 5′-CAGAGGGTTCAGAAACCGCGTTGCTGCAC-3′ |
| GFP-Ub-VPS13DmutR4228Q | Vps13D reverse4228Q reverse | 5′-CGCGGTTTCTGAACCCTCTGTGCTTGAG-3′ |
| GFP-Ub-VPS13DmutS405R | Vps13D_S405R forward | 5′-TTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCAT-3′ |
| GFP-Ub-VPS13DmutS405R | Vps13D_S405R reverse | 5′-AGACATGATCTCGCTTTGTATACGTGTTCATACACGAGGCATC-3′ |
| GFP-Ub-VPS13DmutT1118M | Vps13D-T1118M forward | 5′-AATCCAGAGATGATTGTGGAGCTAATTGG-3′ |
| GFP-Ub-VPS13DmutT1118M | Vps13D-T1118M reverse | 5′-TCCACAATCATCTCTGGATTGAGGATAATATC-3′ |
| GFP-Ub-VPS13DmutT865A | Vps13D-T865A forward | 5′-GATTGATTTATGCTTCAGATCCCAAATATCCAG-3′ |
| GFP-Ub-VPS13DmutT865A | Vps13D-T865A reverse | 5′-GATCTGAAGCATAAATCAATCGACGCTC-3′ |
| pBMNz-GFP-CAT | pBMN-CAT_reverse | 5′-CGCGGTACCGTCGACTGCAGAATTCTCACAGATTTGCCTTCTCCCTTGC-3′ |
| pBMNz-GFP-CAT | pBMN-GFP reverse | 5′-ATAAAATCTTTTATTTTATCGTCGACTTACTTGTACAGCTCGTC-3′ |
| pBMNz-GFP-CAT | pBMNeGFP-CAT_forward | 5′-TACAAGTCCGGACTCAGATCTCGAGGGATGGCTGACAGCCGGGATC-3′ |
| pCDNA-VPS13A/C-mycHis | pcDNA-3xHA-C forward | 5′-ATCCAGCACAGTGGCGGCCGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGG-3′ |
| pCDNA-VPS13A/C-mycHis | pcDNA-3xHA-C reverse | 5′-GGTTTAAACGGGCCCTCTAGACTAAGCGTAATCTGGAACGTCATATGGATAGGAGCCTGCATAGTCCGGGACGTCATAGG-3′ |
| pCDNA-VPS13C-mycHis | Vps13A forward | 5′-TACTCCGTCTCAGAGGTGGGATGGTTTTCGAGTCGGTGGT-3′ |
| pCDNA-VPS13C-mycHis | Vps13A reverse | 5′-ATTATGATCAGTTATCTAGATCAGAGGCTCGGAGAAGGTTCTC-3′ |
| pCDNA-VPS13C-mycHis | Vps13C forward | 5′-TACTCCGTCTCAGAGGTGGGATGGTGCTGGAGTCGGT-3′ |
| pCDNA-VPS13C-mycHis | Vps13C reverse | 5′-ATTATGATCAGTTATCTAGATTTAAGATGGCAATTGGGGTCTGA-3′ |
| pHAGE-IRES-GFP-SKL | IRES-GFP forward | 5′-TGAAAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAG-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-GFP-SKL reverse | 5′-GTAATCCAGAGGTTGATTAGGATCCCTACAGCTTGGAACCGGACTTGTACAGCTCGTCCATGC-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-linker forward | 5′-CCTCCATAGAAGACACCGGCGGCCGCGCTAGCCTCGAGTCTAGAGTCGACACGCGTGAATTCCGCCCCCCCCCCCTAACGT-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-linker reverse | 5′-ACGTTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCTAGACTCGAGGCTAGCGCGGCCGCCGGTGTCTTCTATGGAGG-3′ |
| pHAGE-PEX14-IRES-GFPSKL | pHAGE-PEX14 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGCGTCCTCGGAGCAG-3′ |
| pHAGE-PEX14-IRES-GFPSKL | pHAGE-PEX14 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACCTAGTCCCGCTCACTCTCG-3′ |
| pHAGE-PEX16-IRES-GFPSKL | pHAGE-PEX16 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGAGAAGCTGCGGCTC-3′ |
| pHAGE-PEX16-IRES-GFPSKL | pHAGE-PEX16 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCAGCCCCAACTGTAGAAG-3′ |
| pHAGE-PEX19-IRES-GFPSKL | pHAGE-PEX19 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGCCGCCGCTGAGGAA-3′ |
| pHAGE-PEX19-IRES-GFPSKL | pHAGE-PEX19 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCACATGATCAGACACTGTTCACCAC |
| pHAGE-PEX26-IRES-GFPSKL | pHAGE-PEX26 forward | CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGAAGAGCGATTCTTCGACC-3′ |
| pHAGE-PEX26-IRES-GFPSKL | pHAGE-PEX26 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCAGTCACGGATGCGGAG-3′ |
| pHAGE-PEX3-IRES-GFPSKL | PEX3-IRES reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCATTTCTCCAGTTGCTG-3′ |
| pHAGE-PEX3-IRES-GFPSKL | pHAGE-PEX3 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGCTGAGGTCTGTATGG-3′ |
| Plasmid cloning . | Primer . | Sequence . |
|---|---|---|
| GFP-Ub-VPS13D | EGFP-Ub forward | 5′-TCTAGATAACTGATCATAATCAGCCATAC-3′ |
| GFP-Ub-VPS13D | EGFPC1 forward | 5′-GATCACTCTCGGCATGGAC-3′ |
| GFP-Ub-VPS13D | EGFPC1 reverse | 5′-CATTTTATGTTTCAGGTTCAGGG-3′ |
| GFP-Ub-VPS13D | Ub-Vps13D forward | 5′-TACTCCGTCTCAGAGGTGGGATGTTGGAAGGCCTTGTAGC-3′ |
| GFP-Ub-VPS13D | Ub-Vps13D reverse | 5′-ATTATGATCAGTTATCTAGATCAGGAGTCCAGCTCCAGCTG-3′ |
| GFP-Ub-VPS13DΔC | Ub-Vps13D-dC reverse | 5′-ATTATGATCAGTTATCTAGATCAAATTGGTGTTCCACCTTG-3′ |
| GFP-Ub-VPS13DΔN | Ub-Vps13D-dN forward | 5′-TACTCCGTCTCAGAGGTGGGATGGAGGAGAAATGGAAGAATGACC-3′ |
| GFP-Ub-VPS13DΔN | Ub-Vps13D-dN reverse | 5′-ATTATGATCAGTTATCTAGATCAGGAGTCCAGCTCCAGCTG-3′ |
| GFP-Ub-VPS13DmutG1200D | Vps13D-G1200D forward | 5′-AGTATAGGTGACACCAAAGTTAATGTCTCAATG-3′ |
| GFP-Ub-VPS13DmutG1200D | Vps13D-G1200D reverse | 5′-ACTTTGGTGTCACCTATACTTGCAGTTG-3′ |
| GFP-Ub-VPS13DmutG4149S | Vps13D-G4149S forward | 5′-CCTTGTAGCCAGCATCCATGGCCTGGCTC-3′ |
| GFP-Ub-VPS13DmutG4149S | Vps13D-G4149S reverse | 5′-CATGGATGCTGGCTACAAGGTGTTCACC-3′ |
| GFP-Ub-VPS13DmutL2900S | Vps13D-L2900S forward | 5′-GGGTGCACTTCGTGGTTTGCCACCCTGAC-3′ |
| GFP-Ub-VPS13DmutL2900S | Vps13D-L2900S reverse | 5′-GCAAACCACGAAGTGCACCCCGTGTGGT-3′ |
| GFP-Ub-VPS13DmutN34107I | Vps13D-N4107I forward | 5′-GGAGTATCAATCTCTGCTGCCAAGTTTGC-3′ |
| GFP-Ub-VPS13DmutN34107I | Vps13D-N4107I reverse | 5′-TTGGCAGCAGAGATTGATACTCCGTGTGTAACATTTC-3′ |
| GFP-Ub-VPS13DmutN3521S | Vps13D-N3521S forward | 5′-CGAATTGACAGCTTTTCTAAGGTCCCGGTTG-3′ |
| GFP-Ub-VPS13DmutN3521S | Vps13D-N3521S reverse | 5′-TTAGAAAAGCTGTCAATTCGGAAAGGAG-3′ |
| GFP-Ub-VPS13DmutR4228Q | Vps13D reverse4228Q forward | 5′-CAGAGGGTTCAGAAACCGCGTTGCTGCAC-3′ |
| GFP-Ub-VPS13DmutR4228Q | Vps13D reverse4228Q reverse | 5′-CGCGGTTTCTGAACCCTCTGTGCTTGAG-3′ |
| GFP-Ub-VPS13DmutS405R | Vps13D_S405R forward | 5′-TTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCAT-3′ |
| GFP-Ub-VPS13DmutS405R | Vps13D_S405R reverse | 5′-AGACATGATCTCGCTTTGTATACGTGTTCATACACGAGGCATC-3′ |
| GFP-Ub-VPS13DmutT1118M | Vps13D-T1118M forward | 5′-AATCCAGAGATGATTGTGGAGCTAATTGG-3′ |
| GFP-Ub-VPS13DmutT1118M | Vps13D-T1118M reverse | 5′-TCCACAATCATCTCTGGATTGAGGATAATATC-3′ |
| GFP-Ub-VPS13DmutT865A | Vps13D-T865A forward | 5′-GATTGATTTATGCTTCAGATCCCAAATATCCAG-3′ |
| GFP-Ub-VPS13DmutT865A | Vps13D-T865A reverse | 5′-GATCTGAAGCATAAATCAATCGACGCTC-3′ |
| pBMNz-GFP-CAT | pBMN-CAT_reverse | 5′-CGCGGTACCGTCGACTGCAGAATTCTCACAGATTTGCCTTCTCCCTTGC-3′ |
| pBMNz-GFP-CAT | pBMN-GFP reverse | 5′-ATAAAATCTTTTATTTTATCGTCGACTTACTTGTACAGCTCGTC-3′ |
| pBMNz-GFP-CAT | pBMNeGFP-CAT_forward | 5′-TACAAGTCCGGACTCAGATCTCGAGGGATGGCTGACAGCCGGGATC-3′ |
| pCDNA-VPS13A/C-mycHis | pcDNA-3xHA-C forward | 5′-ATCCAGCACAGTGGCGGCCGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGG-3′ |
| pCDNA-VPS13A/C-mycHis | pcDNA-3xHA-C reverse | 5′-GGTTTAAACGGGCCCTCTAGACTAAGCGTAATCTGGAACGTCATATGGATAGGAGCCTGCATAGTCCGGGACGTCATAGG-3′ |
| pCDNA-VPS13C-mycHis | Vps13A forward | 5′-TACTCCGTCTCAGAGGTGGGATGGTTTTCGAGTCGGTGGT-3′ |
| pCDNA-VPS13C-mycHis | Vps13A reverse | 5′-ATTATGATCAGTTATCTAGATCAGAGGCTCGGAGAAGGTTCTC-3′ |
| pCDNA-VPS13C-mycHis | Vps13C forward | 5′-TACTCCGTCTCAGAGGTGGGATGGTGCTGGAGTCGGT-3′ |
| pCDNA-VPS13C-mycHis | Vps13C reverse | 5′-ATTATGATCAGTTATCTAGATTTAAGATGGCAATTGGGGTCTGA-3′ |
| pHAGE-IRES-GFP-SKL | IRES-GFP forward | 5′-TGAAAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAG-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-GFP-SKL reverse | 5′-GTAATCCAGAGGTTGATTAGGATCCCTACAGCTTGGAACCGGACTTGTACAGCTCGTCCATGC-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-linker forward | 5′-CCTCCATAGAAGACACCGGCGGCCGCGCTAGCCTCGAGTCTAGAGTCGACACGCGTGAATTCCGCCCCCCCCCCCTAACGT-3′ |
| pHAGE-IRES-GFP-SKL | pHAGE-linker reverse | 5′-ACGTTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCTAGACTCGAGGCTAGCGCGGCCGCCGGTGTCTTCTATGGAGG-3′ |
| pHAGE-PEX14-IRES-GFPSKL | pHAGE-PEX14 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGCGTCCTCGGAGCAG-3′ |
| pHAGE-PEX14-IRES-GFPSKL | pHAGE-PEX14 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACCTAGTCCCGCTCACTCTCG-3′ |
| pHAGE-PEX16-IRES-GFPSKL | pHAGE-PEX16 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGAGAAGCTGCGGCTC-3′ |
| pHAGE-PEX16-IRES-GFPSKL | pHAGE-PEX16 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCAGCCCCAACTGTAGAAG-3′ |
| pHAGE-PEX19-IRES-GFPSKL | pHAGE-PEX19 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGGCCGCCGCTGAGGAA-3′ |
| pHAGE-PEX19-IRES-GFPSKL | pHAGE-PEX19 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCACATGATCAGACACTGTTCACCAC |
| pHAGE-PEX26-IRES-GFPSKL | pHAGE-PEX26 forward | CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGAAGAGCGATTCTTCGACC-3′ |
| pHAGE-PEX26-IRES-GFPSKL | pHAGE-PEX26 reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCAGTCACGGATGCGGAG-3′ |
| pHAGE-PEX3-IRES-GFPSKL | PEX3-IRES reverse | 5′-TTAGGGGGGGGGGGCGGAATTCACGCGTGTCGACTCATTTCTCCAGTTGCTG-3′ |
| pHAGE-PEX3-IRES-GFPSKL | pHAGE-PEX3 forward | 5′-CATAGAAGACACCGGCGGCCGCGCTAGCCACCATGCTGAGGTCTGTATGG-3′ |