Table 1.
Comparison of timings in human and mouse preimplantation development.
HumanMouse
First cleavage division 27 to 30 hpf (Mio and Maeda, 2008; Wong et al., 2010; Kirkegaard et al., 2012) 16 to 20 hpf (Arav et al., 2008; Kirkegaard et al., 2012) 
Blastocyst implantation 6 to 8 dpf (Hertig et al., 1956) 4 to 4.5 dpf (Finn and McLaren, 1967) 
Totipotent embryo Up to four-cell (Van de Velde et al., 2008) Up to two-cell (Tarkowski, 1959; Rossant, 1976) 
Major ZGA Four- to eight-cell (Tesarík et al., 1987; Braude et al., 1988; Dobson et al., 2004; Vassena et al., 2011; Yan et al., 2013) Two-cell (Flach et al., 1982; Aoki et al., 1997; Hamatani et al., 2004) 
DNA demethylation Before two-cell (Guo et al., 2014b) In early one-cell (Mayer et al., 2000; Oswald et al., 2000) 
Chromatin accessibility Increases during cleavage stages; detectable promoter accessibility correlates with timing of ZGA (Gao et al., 2018; Li et al., 2018) Increases during cleavage stages; detectable promoter accessibility correlates with timing of ZGA (Lu et al., 2016; Wu et al., 2016) 
Higher-order chromatin structure No data available Gradual establishment during cleavage stages (Du et al., 2017; Flyamer et al., 2017; Gassler et al., 2017; Ke et al., 2017) 
HumanMouse
First cleavage division 27 to 30 hpf (Mio and Maeda, 2008; Wong et al., 2010; Kirkegaard et al., 2012) 16 to 20 hpf (Arav et al., 2008; Kirkegaard et al., 2012) 
Blastocyst implantation 6 to 8 dpf (Hertig et al., 1956) 4 to 4.5 dpf (Finn and McLaren, 1967) 
Totipotent embryo Up to four-cell (Van de Velde et al., 2008) Up to two-cell (Tarkowski, 1959; Rossant, 1976) 
Major ZGA Four- to eight-cell (Tesarík et al., 1987; Braude et al., 1988; Dobson et al., 2004; Vassena et al., 2011; Yan et al., 2013) Two-cell (Flach et al., 1982; Aoki et al., 1997; Hamatani et al., 2004) 
DNA demethylation Before two-cell (Guo et al., 2014b) In early one-cell (Mayer et al., 2000; Oswald et al., 2000) 
Chromatin accessibility Increases during cleavage stages; detectable promoter accessibility correlates with timing of ZGA (Gao et al., 2018; Li et al., 2018) Increases during cleavage stages; detectable promoter accessibility correlates with timing of ZGA (Lu et al., 2016; Wu et al., 2016) 
Higher-order chromatin structure No data available Gradual establishment during cleavage stages (Du et al., 2017; Flyamer et al., 2017; Gassler et al., 2017; Ke et al., 2017) 

dpf, days post-fertilization; hpf, hours post-fertilization.

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