| Plasmid . | Description . | Source/reference . |
|---|---|---|
| BG1805 | 2 µm, URA3, GAL1prom, C-terminal tandem affinity (TAP) tag vector | Open Biosystems |
| pMAL-c5X | Bacterial expression vector for production of MBP (MalE) fusion proteins | New England BioLabs |
| pESC-Leu | 2 µm, GAL1/10prom, LEU2 | Agilent |
| YCpLG | CEN, LEU2, GAL1/10prom vector | Bardwell et al., 1998 |
| pRS425 | 2 µm, LEU2 vector | Sikorski and Hieter, 1989 |
| pRS426 | 2 µm, URA3 vector | Sikorski and Hieter, 1989 |
| pRS315 | CEN, LEU2 vector | Sikorski and Hieter, 1989 |
| pET30a | Bacterial expression vector | Novagen |
| pMLT35 | pMAL-c5X MBP-MUK1-(His)6 | This study |
| pMLT74 | pMAL-c5X MBP-muk1(S171A S172A S173A S183A S184A S185A)-(His)6 | This study |
| pMLT22 | pESC-LEU GAL1prom-MUK1-Myc | This study |
| pMLT56 | pESC-LEU GAL1prom-muk1(1-220)-Myc | This study |
| pMLT57 | pESC-LEU GAL1prom-muk1(1-220; S171A S172A S173A S183A S184A S185A)-Myc | This study |
| pAX50 | BG1805 YPK1(L424A)-TAP | Muir et al., 2014 |
| pFR252 | pRS315 ypk1(S51A T57A S71A T504A S644A S653A T662A)-Myc | Muir et al., 2015 |
| pAM20 | pRS315 Ypk1-myc | Roelants et al., 2011 |
| pMLT101 | pRS426 GAL1prom-FLAG-VPS21 | This study |
| pMLT102 | pRS426 GAL1prom-FLAG-vps21(S21L) | This study |
| pMLT103 | pRS426 GAL1prom-FLAG-vps21(Q66L) | This study |
| pMLT110 | pRS426 GAL1prom-FLAG-YPT7 | This study |
| pMLT114 | pRS426 GAL1prom-FLAG-mNG | This study |
| pMLT111 | pRS426 MSB3-FLAG | This study |
| pMLT83 | pRS315 MUK1-3xFLAG | This study |
| pMLT84 | pRS315 muk1(S171A S172A S173A S183A S184A S185A)-3xFLAG | This study |
| pMLT85 | pRS315 muk1(D353A)-3xFLAG | This study |
| pMLT92 | pRS315 MUK1(S171E S172E S173E S183E S184E S185E)-3xFLAG | This study |
| pMLT132 | pET30a ypk1(D242 D470A)-(His)6 | This study |
| pMLT135 | pMAL-c5X MBP-Ypk1(603-680) [MBP-Ypk1CT] | This study |
| pMLT138 | pMAL-c5X MBP-Ypk1(603-680; S644 S653A T662A S671A S672A S678) [MBP-Ypk1CT (6A)] | This study |
| pMLT88 | pET30a VPS21-(His)6 | This study |
| pMLT141 | pET30a YPT7-(His)6 | This study |
| pPL495 | pRS315 PHSlm1-YPK1-3xHA | Niles et al., 2012 |
| pMLT115 | pRS425 GAL1prom-GST | This study |
| pML116 | pRS425 GAL1prom-GST-Ypk1 | This study |
| Plasmid . | Description . | Source/reference . |
|---|---|---|
| BG1805 | 2 µm, URA3, GAL1prom, C-terminal tandem affinity (TAP) tag vector | Open Biosystems |
| pMAL-c5X | Bacterial expression vector for production of MBP (MalE) fusion proteins | New England BioLabs |
| pESC-Leu | 2 µm, GAL1/10prom, LEU2 | Agilent |
| YCpLG | CEN, LEU2, GAL1/10prom vector | Bardwell et al., 1998 |
| pRS425 | 2 µm, LEU2 vector | Sikorski and Hieter, 1989 |
| pRS426 | 2 µm, URA3 vector | Sikorski and Hieter, 1989 |
| pRS315 | CEN, LEU2 vector | Sikorski and Hieter, 1989 |
| pET30a | Bacterial expression vector | Novagen |
| pMLT35 | pMAL-c5X MBP-MUK1-(His)6 | This study |
| pMLT74 | pMAL-c5X MBP-muk1(S171A S172A S173A S183A S184A S185A)-(His)6 | This study |
| pMLT22 | pESC-LEU GAL1prom-MUK1-Myc | This study |
| pMLT56 | pESC-LEU GAL1prom-muk1(1-220)-Myc | This study |
| pMLT57 | pESC-LEU GAL1prom-muk1(1-220; S171A S172A S173A S183A S184A S185A)-Myc | This study |
| pAX50 | BG1805 YPK1(L424A)-TAP | Muir et al., 2014 |
| pFR252 | pRS315 ypk1(S51A T57A S71A T504A S644A S653A T662A)-Myc | Muir et al., 2015 |
| pAM20 | pRS315 Ypk1-myc | Roelants et al., 2011 |
| pMLT101 | pRS426 GAL1prom-FLAG-VPS21 | This study |
| pMLT102 | pRS426 GAL1prom-FLAG-vps21(S21L) | This study |
| pMLT103 | pRS426 GAL1prom-FLAG-vps21(Q66L) | This study |
| pMLT110 | pRS426 GAL1prom-FLAG-YPT7 | This study |
| pMLT114 | pRS426 GAL1prom-FLAG-mNG | This study |
| pMLT111 | pRS426 MSB3-FLAG | This study |
| pMLT83 | pRS315 MUK1-3xFLAG | This study |
| pMLT84 | pRS315 muk1(S171A S172A S173A S183A S184A S185A)-3xFLAG | This study |
| pMLT85 | pRS315 muk1(D353A)-3xFLAG | This study |
| pMLT92 | pRS315 MUK1(S171E S172E S173E S183E S184E S185E)-3xFLAG | This study |
| pMLT132 | pET30a ypk1(D242 D470A)-(His)6 | This study |
| pMLT135 | pMAL-c5X MBP-Ypk1(603-680) [MBP-Ypk1CT] | This study |
| pMLT138 | pMAL-c5X MBP-Ypk1(603-680; S644 S653A T662A S671A S672A S678) [MBP-Ypk1CT (6A)] | This study |
| pMLT88 | pET30a VPS21-(His)6 | This study |
| pMLT141 | pET30a YPT7-(His)6 | This study |
| pPL495 | pRS315 PHSlm1-YPK1-3xHA | Niles et al., 2012 |
| pMLT115 | pRS425 GAL1prom-GST | This study |
| pML116 | pRS425 GAL1prom-GST-Ypk1 | This study |