Table 1.
S. cerevisiae strains used in this study
StrainGenotypeSource/reference
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Research Genetics 
yAM123A BY4741 Ypk1(L424A)::URA3 ypk2Δ::KanMX4 Muir et al., 2014  
JTY6142 BY4741 ypk1Δ::KanMX4 Research Genetics 
yMLT42 BY4741 vps21Δ::NatMX ypt52Δ::KanMX ypt53Δ::HphMX This study 
yMLT60 BY4741 ypt7Δ::KanMX This study 
yMLT9 BY4741 vps9Δ::HphMX muk1Δ::NatMX This study 
yMLT3 BY4741 vps9Δ::HphMX This study 
yMLT5 BY4741 muk1Δ::NatMX This study 
yMLT78 BY4741 3xHA-TOR2 This study 
yMLT86 BY4741 mup1Δ::HisMX MET15 This study 
yMLT87 BY4741 MET15 This study 
yAEA343 BY4741 vps9Δ::HphMX muk1Δ::NatMX MET15 This study 
yAEA344 BY4741 vps9Δ::HphMX MET15 This study 
yMLT64 BY4741 AVO3-GFP::KAN This study 
yMLT66 BY4741 AVO3-GFP::KAN vps9Δ::HphMX muk1Δ::NatMX This study 
y2470 BY4741 tor1::HIS3 tor2::LEU2 [pRS314(CEN4 TRP1)-3xHA-TOR2Jiang and Broach, 1999  
yMLT85 BY4741 AVO3-3xFLAG::KanMX 3xHA-TOR2 vps21Δ::HphMX ypt52Δ::NatMX This study 
yMLT69 BY4741 AVO1-6xHA::HIS3MX6 This study 
yKL7 BY4741 AVO3-3xFLAG::KanMX TOR2(L2178A)::Hyg K. Leskoske, this laboratory 
yMLT70 BY4741 AVO1-6xHA::HIS3MX6 vps9Δ::HphMX muk1Δ::NatMX This study 
StrainGenotypeSource/reference
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Research Genetics 
yAM123A BY4741 Ypk1(L424A)::URA3 ypk2Δ::KanMX4 Muir et al., 2014  
JTY6142 BY4741 ypk1Δ::KanMX4 Research Genetics 
yMLT42 BY4741 vps21Δ::NatMX ypt52Δ::KanMX ypt53Δ::HphMX This study 
yMLT60 BY4741 ypt7Δ::KanMX This study 
yMLT9 BY4741 vps9Δ::HphMX muk1Δ::NatMX This study 
yMLT3 BY4741 vps9Δ::HphMX This study 
yMLT5 BY4741 muk1Δ::NatMX This study 
yMLT78 BY4741 3xHA-TOR2 This study 
yMLT86 BY4741 mup1Δ::HisMX MET15 This study 
yMLT87 BY4741 MET15 This study 
yAEA343 BY4741 vps9Δ::HphMX muk1Δ::NatMX MET15 This study 
yAEA344 BY4741 vps9Δ::HphMX MET15 This study 
yMLT64 BY4741 AVO3-GFP::KAN This study 
yMLT66 BY4741 AVO3-GFP::KAN vps9Δ::HphMX muk1Δ::NatMX This study 
y2470 BY4741 tor1::HIS3 tor2::LEU2 [pRS314(CEN4 TRP1)-3xHA-TOR2Jiang and Broach, 1999  
yMLT85 BY4741 AVO3-3xFLAG::KanMX 3xHA-TOR2 vps21Δ::HphMX ypt52Δ::NatMX This study 
yMLT69 BY4741 AVO1-6xHA::HIS3MX6 This study 
yKL7 BY4741 AVO3-3xFLAG::KanMX TOR2(L2178A)::Hyg K. Leskoske, this laboratory 
yMLT70 BY4741 AVO1-6xHA::HIS3MX6 vps9Δ::HphMX muk1Δ::NatMX This study 

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