Table 1.
Plasmids used in this study
PlasmidBackboneInsertReference
NES-EGFP-cPHx1 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329) This study 
NES-EGFP-cPHx2 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329) This study 
NES-EGFP-cPHx3 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329) This study 
TagBFP2-CAAX pTagBFP2-C1 TagBFP2:HRAS(172-189) This study 
TagBFP2-INPP4B-CAAX pTagBFP2-C1 TagBFP2:INPP4B:HRAS(172-189) This study 
TagBFP2-INPP4BC842A-CAAX pPAmCherry-C1 TagBFP2:INPP4B(C842A):HRAS(172-189) This study 
NES-PAmCherry-cPHx1 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329) This study 
NES-PAmCherry-cPHx2 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329) This study 
NES-PAmCherry-cPHx3 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329): This study 
NES-mCherry-cPHx3 pNES-mCherry-C1 mCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329) This study 
pNES-iRFP-C1 piRFP-C1 X. laevis map2k1.L(32-44):iRFP This study 
Lyn11-FRB-iRFP piRFP-N1 LYN(1-11):MTOR(2021-2113):iRFP Hammond et al. (2014)  
LAMP1-FRB-iRFP piRFP-N1 LAMP1:MTOR(2021-2113):iRFP This study 
mCherry-FKBP-INPP5E pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP5E(214-644) Hammond et al. (2014)  
mCherry-FKBP-INPP5ED556A pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP5E(214-644) Hammond et al. (2014)  
mCherry-FKBP-INPP4B pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP4B This study 
mCherry-FKBP-INPP4BC842A pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP4B(C842A) This study 
mCherry-FKBP-PTEN pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:PTEN This study 
TagBFP2-FKBP-iSH2 pTagBFP2 TagBFP2:AAAGAGGAA:FKBP1A(3-108): [GGSA]4GG:Mus musculus Pik3r1(159-349) Suh et al. (2006)  
NES-EGFP-P4Mx1 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:L. pneumophila SidM(546-647) Zewe et al. (2018)  
PH-PLCD1-EGFP pEGFP-N1 PLCD1v2(1:170):EGFP Várnai and Balla (1998)  
PH-AKT-EGFP pEGFP-N1 AKT(1-164):EGFP Servant et al. (2000)  
NES-EGFP-PH-ARNO2Gx2 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:CYTH2(252-399):GGSGGVDM: CYTH2(252-399) This study 
NES-EGFP-PH-ARNO2G-I303Ex2 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:CYTH2(252-399)(I303E):GGSGGVDM: CYTH2(252-399)(I303E) This study 
EGF-FYVE-EEA1 pEGFP-C1 EGFP:EEA1(1253-1411) Balla et al. (2000)  
pcDNA3-U6H1-X1-HCKRS pcDNA3 pyrrolysyl-tRNA synthetase/tRNA Luo et al. (2014)  
TagBFP2-LepBK39TAG-iRFP pTagBFP2-C1 TagBFP2: L. pneumophila LepBK39TAG(1-311):GGSGG:iRFP This study 
mCherry-Rab5 pmCherry-C1 mCherry:Canis lupus RAB5A Hammond et al. (2014)  
mCherry-PI3K-C2α pmCherry-C1 mCherry:PIK3C2A Posor et al. (2013)  
mCherry-cPH-CTx2-Aux1 pmCherry-C1 mCherry:[GGS]5:PLEKHA1(180-404):EF:PLEKHA1(180-404):YRYFQAS:Bos Taurus DNAJC6(420-814) He et al. (2017)  
pCherry-cPHx3-Aux1 pmCherry-C1 mCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329):RVDGTAEAS: Bos Taurus DNAJC6(420-814) This study 
pmCherry-CTx2-Aux1 pmCherry-C1 mCherry:[GGS]3: PLEKHA1(329-404):GGSGGSGG: PLEKHA1(329-404):QAS: Bos Taurus DNAJC6(420-814) This study 
pmCherry-PH-PLCδ1-Aux1 pmCherry-C1 mCherry:[GGS]3:PLCD1v2(2-175):QAS: Bos Taurus DNAJC6(420-814) He et al. (2017)  
PlasmidBackboneInsertReference
NES-EGFP-cPHx1 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329) This study 
NES-EGFP-cPHx2 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329) This study 
NES-EGFP-cPHx3 pNES-EGFP-C1 X. laevis map2k1.L(32-44):EGFP:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329) This study 
TagBFP2-CAAX pTagBFP2-C1 TagBFP2:HRAS(172-189) This study 
TagBFP2-INPP4B-CAAX pTagBFP2-C1 TagBFP2:INPP4B:HRAS(172-189) This study 
TagBFP2-INPP4BC842A-CAAX pPAmCherry-C1 TagBFP2:INPP4B(C842A):HRAS(172-189) This study 
NES-PAmCherry-cPHx1 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329) This study 
NES-PAmCherry-cPHx2 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329) This study 
NES-PAmCherry-cPHx3 pPAmCherry-C1 PAmCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329): This study 
NES-mCherry-cPHx3 pNES-mCherry-C1 mCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329) This study 
pNES-iRFP-C1 piRFP-C1 X. laevis map2k1.L(32-44):iRFP This study 
Lyn11-FRB-iRFP piRFP-N1 LYN(1-11):MTOR(2021-2113):iRFP Hammond et al. (2014)  
LAMP1-FRB-iRFP piRFP-N1 LAMP1:MTOR(2021-2113):iRFP This study 
mCherry-FKBP-INPP5E pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP5E(214-644) Hammond et al. (2014)  
mCherry-FKBP-INPP5ED556A pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP5E(214-644) Hammond et al. (2014)  
mCherry-FKBP-INPP4B pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP4B This study 
mCherry-FKBP-INPP4BC842A pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:INPP4B(C842A) This study 
mCherry-FKBP-PTEN pmCherry-C1 mCherry:FKBP1A(3-108):[GGSA]4GG:PTEN This study 
TagBFP2-FKBP-iSH2 pTagBFP2 TagBFP2:AAAGAGGAA:FKBP1A(3-108): [GGSA]4GG:Mus musculus Pik3r1(159-349) Suh et al. (2006)  
NES-EGFP-P4Mx1 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:L. pneumophila SidM(546-647) Zewe et al. (2018)  
PH-PLCD1-EGFP pEGFP-N1 PLCD1v2(1:170):EGFP Várnai and Balla (1998)  
PH-AKT-EGFP pEGFP-N1 AKT(1-164):EGFP Servant et al. (2000)  
NES-EGFP-PH-ARNO2Gx2 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:CYTH2(252-399):GGSGGVDM: CYTH2(252-399) This study 
NES-EGFP-PH-ARNO2G-I303Ex2 pEGFP-C1 X. laevis map2k1.L(32-44):EGFP:CYTH2(252-399)(I303E):GGSGGVDM: CYTH2(252-399)(I303E) This study 
EGF-FYVE-EEA1 pEGFP-C1 EGFP:EEA1(1253-1411) Balla et al. (2000)  
pcDNA3-U6H1-X1-HCKRS pcDNA3 pyrrolysyl-tRNA synthetase/tRNA Luo et al. (2014)  
TagBFP2-LepBK39TAG-iRFP pTagBFP2-C1 TagBFP2: L. pneumophila LepBK39TAG(1-311):GGSGG:iRFP This study 
mCherry-Rab5 pmCherry-C1 mCherry:Canis lupus RAB5A Hammond et al. (2014)  
mCherry-PI3K-C2α pmCherry-C1 mCherry:PIK3C2A Posor et al. (2013)  
mCherry-cPH-CTx2-Aux1 pmCherry-C1 mCherry:[GGS]5:PLEKHA1(180-404):EF:PLEKHA1(180-404):YRYFQAS:Bos Taurus DNAJC6(420-814) He et al. (2017)  
pCherry-cPHx3-Aux1 pmCherry-C1 mCherry:PLEKHA1(169-329):GGSGGSGG: PLEKHA1(169-329): GGSGGSGG: PLEKHA1(169-329):RVDGTAEAS: Bos Taurus DNAJC6(420-814) This study 
pmCherry-CTx2-Aux1 pmCherry-C1 mCherry:[GGS]3: PLEKHA1(329-404):GGSGGSGG: PLEKHA1(329-404):QAS: Bos Taurus DNAJC6(420-814) This study 
pmCherry-PH-PLCδ1-Aux1 pmCherry-C1 mCherry:[GGS]3:PLCD1v2(2-175):QAS: Bos Taurus DNAJC6(420-814) He et al. (2017)  
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