Table 2.
Primers used for the assembly of the respective pCFD6 construct gRNAs
PrimerSequence (5′ to 3′)
sd_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAC​CGC​TGA​TGC​CGA​AGG​TGT​AGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
sd_PCR1R CAC​GGC​GGA​TAT​ATA​GAT​AAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
sd_PCR2F TTA​TCT​ATA​TAT​CCG​CCG​TGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
sd_PCR2R TAC​ATT​TTA​CCC​TCG​TCG​GAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
sd_PCR3F TCC​GAC​GAG​GGT​AAA​ATG​TAG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
sd_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​GCA​TTC​ACT​GGT​GTC​CAC​GGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAA​TTC​GAC​AGC​GTC​CTG​AAT​CGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
yki_PCR1R GAA​TCC​GGG​TGA​CGC​CAA​GCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR2F GCT​TGG​CGT​CAC​CCG​GAT​TCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
yki_PCR2R CGT​CGT​AGG​TGG​AGT​CGG​CGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR3F CGC​CGA​CTC​CAC​CTA​CGA​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
yki_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​CCT​ACG​ACG​CGG​GCT​CCC​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAA​CTG​AAA​GTC​CAG​TGA​CAT​GGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
Jra_PCR1R GAT​CTG​TCA​TCC​AAG​ACG​GTT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR2F ACC​GTC​TTG​GAT​GAC​AGA​TCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Jra_PCR2R CGA​ATC​CTA​ACA​AGC​GTC​CCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR3F GGG​ACG​CTT​GTT​AGG​ATT​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Jra_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​ACG​GGC​TCG​GTT​TTA​GGT​ATT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAT​CGT​CAG​TGT​GAG​AAC​ACT​CGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
kay_PCR1R CGC​GCA​ACA​TCG​AGG​ACA​CAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR2F TGT​GTC​CTC​GAT​GTT​GCG​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
kay_PCR2R CTT​GCT​CTC​GGA​CAC​GCA​GAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR3F TCT​GCG​TGT​CCG​AGA​GCA​AGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
kay_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​GCG​CAA​CCA​GCC​ACA​CGA​TCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAT​GGT​CGT​CCA​TTA​TGG​AAT​AGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
Myc_PCR1R TTC​AGA​GTG​ATC​TCG​AGA​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR2F CTT​CTC​GAG​ATC​ACT​CTG​AAG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Myc_PCR2R GGC​GGT​ATT​AAA​TGG​ACC​TCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR3F GAG​GTC​CAT​TTA​ATA​CCG​CCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Myc_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​CCG​ACG​ACC​GGC​TCT​GAT​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
PrimerSequence (5′ to 3′)
sd_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAC​CGC​TGA​TGC​CGA​AGG​TGT​AGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
sd_PCR1R CAC​GGC​GGA​TAT​ATA​GAT​AAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
sd_PCR2F TTA​TCT​ATA​TAT​CCG​CCG​TGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
sd_PCR2R TAC​ATT​TTA​CCC​TCG​TCG​GAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
sd_PCR3F TCC​GAC​GAG​GGT​AAA​ATG​TAG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
sd_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​GCA​TTC​ACT​GGT​GTC​CAC​GGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAA​TTC​GAC​AGC​GTC​CTG​AAT​CGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
yki_PCR1R GAA​TCC​GGG​TGA​CGC​CAA​GCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR2F GCT​TGG​CGT​CAC​CCG​GAT​TCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
yki_PCR2R CGT​CGT​AGG​TGG​AGT​CGG​CGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
yki_PCR3F CGC​CGA​CTC​CAC​CTA​CGA​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
yki_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​CCT​ACG​ACG​CGG​GCT​CCC​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAA​CTG​AAA​GTC​CAG​TGA​CAT​GGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
Jra_PCR1R GAT​CTG​TCA​TCC​AAG​ACG​GTT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR2F ACC​GTC​TTG​GAT​GAC​AGA​TCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Jra_PCR2R CGA​ATC​CTA​ACA​AGC​GTC​CCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Jra_PCR3F GGG​ACG​CTT​GTT​AGG​ATT​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Jra_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​ACG​GGC​TCG​GTT​TTA​GGT​ATT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAT​CGT​CAG​TGT​GAG​AAC​ACT​CGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
kay_PCR1R CGC​GCA​ACA​TCG​AGG​ACA​CAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR2F TGT​GTC​CTC​GAT​GTT​GCG​CGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
kay_PCR2R CTT​GCT​CTC​GGA​CAC​GCA​GAT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
kay_PCR3F TCT​GCG​TGT​CCG​AGA​GCA​AGG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
kay_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​GCG​CAA​CCA​GCC​ACA​CGA​TCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR1F CGG​CCC​GGG​TTC​GAT​TCC​CGG​CCG​ATG​CAT​GGT​CGT​CCA​TTA​TGG​AAT​AGT​TTC​AGA​GCT​ATG​CTG​GAA​AC 
Myc_PCR1R TTC​AGA​GTG​ATC​TCG​AGA​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR2F CTT​CTC​GAG​ATC​ACT​CTG​AAG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Myc_PCR2R GGC​GGT​ATT​AAA​TGG​ACC​TCT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
Myc_PCR3F GAG​GTC​CAT​TTA​ATA​CCG​CCG​TTT​CAG​AGC​TAT​GCT​GGA​AAC 
Myc_PCR3R ATT​TTA​ACT​TGC​TAT​TTC​TAG​CTC​TAA​AAC​CCG​ACG​ACC​GGC​TCT​GAT​AGT​GCA​CCA​GCC​GGG​AAT​CGA​ACC 
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