Table 1. The guide sequences used for... | Rockefeller University Press

Table 1.

The guide sequences used for gene editing by CRISPR-Cas9

Gene	CRISPR-Cas9 targets (PAM)	Allele
vps-34	5′-CCAGGTATGCGGGCGACACCGAC-3′	qx477
	5′-ACCTGTTCAGATATGATTCCAGG-3′	qx467
	5′-ATATCTACGATCAGTTAATTGGG-3′	qx456
bec-1	5′-ATCCCGTGAAATCACTAAATAGG-3′	qx512
ubq-2	5′-AATCTTCGTCAAGACTCTGACGG-3′	qx473
vps-15	5′-ACTTTTCACAACGAATCCGTCGG-3′	qx523
	5′-CTCGTCTCTTCTGGCTACGATGG-3′	qx525

Gene	CRISPR-Cas9 targets (PAM)	Allele
vps-34	5′-CCAGGTATGCGGGCGACACCGAC-3′	qx477
	5′-ACCTGTTCAGATATGATTCCAGG-3′	qx467
	5′-ATATCTACGATCAGTTAATTGGG-3′	qx456
bec-1	5′-ATCCCGTGAAATCACTAAATAGG-3′	qx512
ubq-2	5′-AATCTTCGTCAAGACTCTGACGG-3′	qx473
vps-15	5′-ACTTTTCACAACGAATCCGTCGG-3′	qx523
	5′-CTCGTCTCTTCTGGCTACGATGG-3′	qx525

The protospacer adjacent motif (PAM) sites are in bold.