Table 2.
List of antibodies used
AntibodyReferenceWorking dilution
Mouse anti-γH2AX Abcam (ab22551) Western blot and IF (1:1,000) 
Rabbit anti-GANP Abcam (113295) Western blot 1:500 
Rabbit anti 53BP1 Novus Biologicals (NB100-304) IF (1:1,000) 
Rabbit anti-Phospho RPA32 (S4/8) Bethyl (A300-245A) IF (1:500) 
Rabbit anti-Phospho RPA 32 (S33) Bethyl (A300-246A) IF (1:1,000) 
Rabbit anti-RAD51 EMD Millipore (PC130) IF (1:500) Western blot (1:1,000) 
Mouse anti-ATM pS1981 Rockland (200-301-400) Western blot (1:2,000) 
Rabbit anti-ENY2 Santa Cruz Biotechnology (sc-87712) Western blot (1:200) 
Mouse anti-γTUBULIN Sigma-Aldrich (T6557) Western blot (1:1,000) 
Rabbit anti-phospho KAP1 Bethyl (A300-767A) Western blot (1:500) 
Mouse anti-GAPDH EMD Millipore (MAB374) Western blot (1:1,000) 
Rabbit anti-BRCA1 Santa Cruz Biotechnology(C-20 SC642) IF (1:100) 
Rabbit anti-CtIP Bethyl (A300-488A) IF (1:100) 
Rabbit anti-H2Bub1 Cell Signaling Technology (CS55465) Western blot (1:10,000) 
Mouse anti-H2B H2-2A4 (Bonnet et al., 2010) Western blot (1:10,000) 
Mouse anti-TBP 3G3 (Brou et al., 1993) Western blot (1:1,000) 
Mouse anti-αTUBULIN Sigma-Aldrich (T9026) Western blot (1:1,000) 
Mouse anti-γH2AX EMD Millipore (05-636) ChIP (2 µg/50 µg of chromatin) 
Mouse anti-H2Bub1 Medimab ChIP (4 µg/50 µg of chromatin) 
Mouse anti-RPA Genetex (GTX22175) IF (1:500) 
AntibodyReferenceWorking dilution
Mouse anti-γH2AX Abcam (ab22551) Western blot and IF (1:1,000) 
Rabbit anti-GANP Abcam (113295) Western blot 1:500 
Rabbit anti 53BP1 Novus Biologicals (NB100-304) IF (1:1,000) 
Rabbit anti-Phospho RPA32 (S4/8) Bethyl (A300-245A) IF (1:500) 
Rabbit anti-Phospho RPA 32 (S33) Bethyl (A300-246A) IF (1:1,000) 
Rabbit anti-RAD51 EMD Millipore (PC130) IF (1:500) Western blot (1:1,000) 
Mouse anti-ATM pS1981 Rockland (200-301-400) Western blot (1:2,000) 
Rabbit anti-ENY2 Santa Cruz Biotechnology (sc-87712) Western blot (1:200) 
Mouse anti-γTUBULIN Sigma-Aldrich (T6557) Western blot (1:1,000) 
Rabbit anti-phospho KAP1 Bethyl (A300-767A) Western blot (1:500) 
Mouse anti-GAPDH EMD Millipore (MAB374) Western blot (1:1,000) 
Rabbit anti-BRCA1 Santa Cruz Biotechnology(C-20 SC642) IF (1:100) 
Rabbit anti-CtIP Bethyl (A300-488A) IF (1:100) 
Rabbit anti-H2Bub1 Cell Signaling Technology (CS55465) Western blot (1:10,000) 
Mouse anti-H2B H2-2A4 (Bonnet et al., 2010) Western blot (1:10,000) 
Mouse anti-TBP 3G3 (Brou et al., 1993) Western blot (1:1,000) 
Mouse anti-αTUBULIN Sigma-Aldrich (T9026) Western blot (1:1,000) 
Mouse anti-γH2AX EMD Millipore (05-636) ChIP (2 µg/50 µg of chromatin) 
Mouse anti-H2Bub1 Medimab ChIP (4 µg/50 µg of chromatin) 
Mouse anti-RPA Genetex (GTX22175) IF (1:500) 

IF, immunofluorescence.

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