Table 1. Neuro-c medium composition Item... | Rockefeller University Press

Table 1.

Neuro-c medium composition

Item	Concentration
	mM
Amino acids
Glycine	0.40
l-Alanine	0.02
l-Arginine	0.40
l-Asparagine	0.01
l-Cysteine	0.26
l-Histidine	0.20
l-Isoleucine	0.80^{^a}
l-Leucine	0.8–2.0^{^a}
l-Lysine	0.80
l-Methionine	0.20
l-Phenylalanine	0.40
l-Proline	0.07
l-Serine	0.40
l-Threonine	0.80
l-Tryptophan	0.08
l-Tyrosine	0.40
l-Valine	0.80^{^a}
Vitamins
Choline chloride	0.029
d-Calcium pantothenate	0.008
Folic acid	0.009
Niacinamide	0.033
Pyridoxal hydrochloride	0.02
Riboflavin	0.001
Thiamine hydrochloride	0.012
Vitamin B12	5.02 × 10⁻⁶
i-Inositol	0.04
Inorganic salts
Calcium chloride	1.801
Ferric nitrate	2.48 × 10⁻⁴
Magnesium chloride	0.814
Potassium chloride	5.333
Sodium chloride	51.724
Sodium phosphate (monobasic)	0.906
Zinc sulfate	6.74 × 10⁻⁴
Additives
Sodium bicarbonate	26.19 mM^{^a}
Hepes	5-10.9 mM^{^a}
Phenol red	0.02 mM
B27 supplements	2% (vol/vol)^{^a}
GlutaMAX	2 mM^{^a}
Penicillin	100 U/ml^{^a}
Streptomycin	100 µg/ml^{^a}
l-Glutamine	0.5–2.0 mM^{^a}
Glucose	8–10 mM^{^a}
Pyruvate	0.22–1.0 mM^{^a}
β-Hydroxybutyrate	2–3 mM^{^a}

Item	Concentration
	mM
Amino acids
Glycine	0.40
l-Alanine	0.02
l-Arginine	0.40
l-Asparagine	0.01
l-Cysteine	0.26
l-Histidine	0.20
l-Isoleucine	0.80^{^a}
l-Leucine	0.8–2.0^{^a}
l-Lysine	0.80
l-Methionine	0.20
l-Phenylalanine	0.40
l-Proline	0.07
l-Serine	0.40
l-Threonine	0.80
l-Tryptophan	0.08
l-Tyrosine	0.40
l-Valine	0.80^{^a}
Vitamins
Choline chloride	0.029
d-Calcium pantothenate	0.008
Folic acid	0.009
Niacinamide	0.033
Pyridoxal hydrochloride	0.02
Riboflavin	0.001
Thiamine hydrochloride	0.012
Vitamin B12	5.02 × 10⁻⁶
i-Inositol	0.04
Inorganic salts
Calcium chloride	1.801
Ferric nitrate	2.48 × 10⁻⁴
Magnesium chloride	0.814
Potassium chloride	5.333
Sodium chloride	51.724
Sodium phosphate (monobasic)	0.906
Zinc sulfate	6.74 × 10⁻⁴
Additives
Sodium bicarbonate	26.19 mM^{^a}
Hepes	5-10.9 mM^{^a}
Phenol red	0.02 mM
B27 supplements	2% (vol/vol)^{^a}
GlutaMAX	2 mM^{^a}
Penicillin	100 U/ml^{^a}
Streptomycin	100 µg/ml^{^a}
l-Glutamine	0.5–2.0 mM^{^a}
Glucose	8–10 mM^{^a}
Pyruvate	0.22–1.0 mM^{^a}
β-Hydroxybutyrate	2–3 mM^{^a}

Added as indicated further in the text.