| . | FiloDetect (Nilufar et al., 2013 ) . | CellGeo (Tsygankov et al., 2014 ) . | ADAPT (Barry et al., 2015 ) . | FiloQuant (this study) . |
|---|---|---|---|---|
| Requirement for proprietary software | Yes (MATLAB) | Yes (MATLAB) | No (ImageJ) | No (ImageJ) |
| Analysis of single images | Yes | No | No | Yes |
| Analysis of movies/filopodia tracking | No | Yes | Yes | Yes in combination with a freely available ImageJ plugin |
| Optimized for single cells | Yes | Yes | Yes | Yes |
| Optimized for cell sheets | No | No | No | Yes |
| Quantification of filopodia length and number | Yes | Yes | Yes | Yes |
| Quantification of edge length to measure filopodia density | No | Yes (indirectly) | No | Yes |
| Options to improve faint filopodia detection | No | No | No | Yes |
| Option to filter filopodia in function of size | No | Yes | Yes | Yes |
| Option to filter filopodia in function of proximity to the cell or colony edge | No | No | No | Yes |
| Batch mode available | No | Yes | Yes | Yes |
| Customizable code (easily) | No | No | No | Yes |
| Easy access to update | No | No | Yes | Yes |
| Analysis of lamellipodia and/or blebs | No | Yes | Yes | No |
| . | FiloDetect (Nilufar et al., 2013 ) . | CellGeo (Tsygankov et al., 2014 ) . | ADAPT (Barry et al., 2015 ) . | FiloQuant (this study) . |
|---|---|---|---|---|
| Requirement for proprietary software | Yes (MATLAB) | Yes (MATLAB) | No (ImageJ) | No (ImageJ) |
| Analysis of single images | Yes | No | No | Yes |
| Analysis of movies/filopodia tracking | No | Yes | Yes | Yes in combination with a freely available ImageJ plugin |
| Optimized for single cells | Yes | Yes | Yes | Yes |
| Optimized for cell sheets | No | No | No | Yes |
| Quantification of filopodia length and number | Yes | Yes | Yes | Yes |
| Quantification of edge length to measure filopodia density | No | Yes (indirectly) | No | Yes |
| Options to improve faint filopodia detection | No | No | No | Yes |
| Option to filter filopodia in function of size | No | Yes | Yes | Yes |
| Option to filter filopodia in function of proximity to the cell or colony edge | No | No | No | Yes |
| Batch mode available | No | Yes | Yes | Yes |
| Customizable code (easily) | No | No | No | Yes |
| Easy access to update | No | No | Yes | Yes |
| Analysis of lamellipodia and/or blebs | No | Yes | Yes | No |