| Topic . | Resources . |
|---|---|
| Microscopy texts | • Book: Video Microscopy, Inoué and Spring, 1997 |
| • Book: Handbook of Biological Confocal Microscopy, Pawley, 2006b | |
| • Book: Fundamentals of Light Microscopy and Electronic Imaging, Murphy and Davidson, 2012 | |
| Microscopy courses | • A comprehensive listing of microscopy courses can be found at https://www.microlist.org |
| • iBiology Microscopy Series (https://www.ibiology.org/online-biology-courses/microscopy-series/; a free online microscopy course) | |
| • Microcourses YouTube channel: Short educational videos on microscopy | |
| • Course: Quantitative Imaging: From Acquisition to Analysis, Cold Spring Harbor Laboratory, NY | |
| • Courses and workshops offered by the European Molecular Biology Organization in Europe | |
| • Courses and workshops offered by the Royal Microscopical Society in the United Kingdom | |
| • Course: Bangalore Microscopy Course, National Centre For Biological Sciences, Bangalore, India | |
| Quantitative microscopy | • Review: Waters, 2009. An introduction to sources of error in microscopy |
| • Review: Wolf et al., 2013. Protocols for error detection and correction | |
| • Book: Quantitative Imaging in Cell Biology, Waters and Wittmann, 2014 | |
| Live-cell imaging | • Review: Ettinger and Wittmann, 2014. A practical introduction to fluorescence live cell imaging |
| • Review: Magidson and Khodjakov, 2013. Strategies for reducing photodamage | |
| • Book: Live Cell Imaging: A Laboratory Manual, Goldman et al., 2010 | |
| Colocalization | • Review: Bolte and Cordelières, 2006. Validation and error correction in colocalization image acquisition and analysis |
| Ratiometric imaging, including Förster Resonance Energy Transfer (FRET) | • Reviews: Hodgson et al., 2010; O’Connor and Silver, 2013; Spiering et al., 2013; Grillo-Hill et al., 2014. Validation and error correction in image acquisition and analysis of ratiometric probes |
| Fluorescence Recovery After Photobleaching (FRAP) | • Reviews: Phair et al., 2003; Bancaud et al., 2010. Protocols for acquisition, correction, and analysis of FRAP data |
| Single-molecule imaging | • Book: Single-Molecule Techniques: A Laboratory Manual, Selvin and Ha, 2008 |
| Specimen preparation | • Book: Protein Localization by Fluorescence Microscopy: A Practical Approach, Allan, 2000 |
| • Review: Shaner, 2014. Protocol for testing FPs in mammalian cells | |
| • Review: Rodriguez et al., 2017. Summary of recent developments and new directions in the FP field | |
| • Website: http://fpbase.org. For choosing and comparing FPs | |
| Image analysis | • Review: Eliceiri et al., 2012. A general review of steps in image analysis; also includes options for free and open-source image analysis software |
| • Website: https://image.sc. An online forum for questions and discussions on image analysis and open source software | |
| • eBook: Analyzing Fluorescence Microscopy Images With ImageJ, Bankhead, 2016 | |
| Experimental design | • Book: Experimental Design for Laboratory Biologists: Maximizing Information and Improving Reproducibility, Lazic, 2016 |
| • Book: Experimental Design for Biologists, Glass, 2014 |
| Topic . | Resources . |
|---|---|
| Microscopy texts | • Book: Video Microscopy, Inoué and Spring, 1997 |
| • Book: Handbook of Biological Confocal Microscopy, Pawley, 2006b | |
| • Book: Fundamentals of Light Microscopy and Electronic Imaging, Murphy and Davidson, 2012 | |
| Microscopy courses | • A comprehensive listing of microscopy courses can be found at https://www.microlist.org |
| • iBiology Microscopy Series (https://www.ibiology.org/online-biology-courses/microscopy-series/; a free online microscopy course) | |
| • Microcourses YouTube channel: Short educational videos on microscopy | |
| • Course: Quantitative Imaging: From Acquisition to Analysis, Cold Spring Harbor Laboratory, NY | |
| • Courses and workshops offered by the European Molecular Biology Organization in Europe | |
| • Courses and workshops offered by the Royal Microscopical Society in the United Kingdom | |
| • Course: Bangalore Microscopy Course, National Centre For Biological Sciences, Bangalore, India | |
| Quantitative microscopy | • Review: Waters, 2009. An introduction to sources of error in microscopy |
| • Review: Wolf et al., 2013. Protocols for error detection and correction | |
| • Book: Quantitative Imaging in Cell Biology, Waters and Wittmann, 2014 | |
| Live-cell imaging | • Review: Ettinger and Wittmann, 2014. A practical introduction to fluorescence live cell imaging |
| • Review: Magidson and Khodjakov, 2013. Strategies for reducing photodamage | |
| • Book: Live Cell Imaging: A Laboratory Manual, Goldman et al., 2010 | |
| Colocalization | • Review: Bolte and Cordelières, 2006. Validation and error correction in colocalization image acquisition and analysis |
| Ratiometric imaging, including Förster Resonance Energy Transfer (FRET) | • Reviews: Hodgson et al., 2010; O’Connor and Silver, 2013; Spiering et al., 2013; Grillo-Hill et al., 2014. Validation and error correction in image acquisition and analysis of ratiometric probes |
| Fluorescence Recovery After Photobleaching (FRAP) | • Reviews: Phair et al., 2003; Bancaud et al., 2010. Protocols for acquisition, correction, and analysis of FRAP data |
| Single-molecule imaging | • Book: Single-Molecule Techniques: A Laboratory Manual, Selvin and Ha, 2008 |
| Specimen preparation | • Book: Protein Localization by Fluorescence Microscopy: A Practical Approach, Allan, 2000 |
| • Review: Shaner, 2014. Protocol for testing FPs in mammalian cells | |
| • Review: Rodriguez et al., 2017. Summary of recent developments and new directions in the FP field | |
| • Website: http://fpbase.org. For choosing and comparing FPs | |
| Image analysis | • Review: Eliceiri et al., 2012. A general review of steps in image analysis; also includes options for free and open-source image analysis software |
| • Website: https://image.sc. An online forum for questions and discussions on image analysis and open source software | |
| • eBook: Analyzing Fluorescence Microscopy Images With ImageJ, Bankhead, 2016 | |
| Experimental design | • Book: Experimental Design for Laboratory Biologists: Maximizing Information and Improving Reproducibility, Lazic, 2016 |
| • Book: Experimental Design for Biologists, Glass, 2014 |