| Injured . | 12 d after injury . | 28 d after injury . | ||
|---|---|---|---|---|
| Ctrl . | cRalA/B−/− . | Ctrl . | cRalA/B−/− . | |
| Myelinated axons × 1,000/mm2 | 7.15 ± 0.72 | 7.65 ± 0.26 | 19.10 ± 1.70 | 16.28 ± 1.60 |
| Unmyelinated axons × 1,000/mm2 | 46.78 ± 8.66 | 48.68 ± 4.18 | 50.33 ± 12.81 | 30.15 ± 6.73 |
| % nude axons >1 µm | 12.55 ± 1.21 | 25.71 ± 2.30* | 4.57 ± 0.64 | 9.56 ± 1.18* |
| g-ratio | 0.845 ± 0.005 | 0.879 ± 0.006* | 0.657 ± 0.009 | 0.693 ± 0.008* |
| Remak bundles × 1,000/mm2 | 6.25 ± 1.84 | 5.35 ± 0.81 | 6.63 ± 0.87 | 6.85 ± 0.30 |
| % bundles with polyaxonal pockets | 39.45 ± 2.91 | 50.98 ± 3.83 | 14.75 ± 3.55 | 15.92 ± 2.47 |
| C-fibers/Remak bundle | 8.39 ± 0.85 | 7.91 ± 0.42 | 5.8 ± 0.30 | 6.12 ± 0.60 |
| Injured . | 12 d after injury . | 28 d after injury . | ||
|---|---|---|---|---|
| Ctrl . | cRalA/B−/− . | Ctrl . | cRalA/B−/− . | |
| Myelinated axons × 1,000/mm2 | 7.15 ± 0.72 | 7.65 ± 0.26 | 19.10 ± 1.70 | 16.28 ± 1.60 |
| Unmyelinated axons × 1,000/mm2 | 46.78 ± 8.66 | 48.68 ± 4.18 | 50.33 ± 12.81 | 30.15 ± 6.73 |
| % nude axons >1 µm | 12.55 ± 1.21 | 25.71 ± 2.30* | 4.57 ± 0.64 | 9.56 ± 1.18* |
| g-ratio | 0.845 ± 0.005 | 0.879 ± 0.006* | 0.657 ± 0.009 | 0.693 ± 0.008* |
| Remak bundles × 1,000/mm2 | 6.25 ± 1.84 | 5.35 ± 0.81 | 6.63 ± 0.87 | 6.85 ± 0.30 |
| % bundles with polyaxonal pockets | 39.45 ± 2.91 | 50.98 ± 3.83 | 14.75 ± 3.55 | 15.92 ± 2.47 |
| C-fibers/Remak bundle | 8.39 ± 0.85 | 7.91 ± 0.42 | 5.8 ± 0.30 | 6.12 ± 0.60 |
Quantification of the total number of myelinated and non-myelinated axons per sciatic nerve cross-section at 12 and 28 d after crush does not show any difference between mice. Quantification of non-myelinated axons of >1 µm diameter at 12 and 30 d after injury shows a clear increase in the percentage of non-myelinated axons of >1 µm diameter at both time points (*, P < 0.05, Student’s t test). Number of animals = 4. Number of quantified fibers per animal at 12 d = 50. Number of quantified fibers per animal at 1 mo = 150. Quantification of g-ratios 12 and 28 d after crush shows a significant increase in the g-ratio of the cRalA/B−/− mice compared to control littermates at both time points (*, P < 0.05, Student’s t test, n = 4). Remak bundles, percentage of Remak bundles with polyaxonal pockets and number of C-fibers per Remak bundle, 12 d and 1 mo after injury, did not show any difference.