Table 1. Summary of observations... | Rockefeller University Press

Table 1.

Summary of observations

Condition	Spindle	Attach	Tension	SAC	Dam1C/DASH rings			kMT
					Complete	Partial	n	nm
Metaphase	Intact	+	+	OFF	39%	61%	46	50–200+
Nocodazole treatment	MT stubs	+	−	ON	86%	14%	7	20–50
Nocodazole treatment	Absent	−	−	ON	0%	100%	37	n/a
Metaphase, Scc1 cleaved; Scc1-TEV268	Distorted	+	−	ON	39%	61%	33	50–200+

Condition	Spindle	Attach	Tension	SAC	Dam1C/DASH rings			kMT
					Complete	Partial	n	nm
Metaphase	Intact	+	+	OFF	39%	61%	46	50–200+
Nocodazole treatment	MT stubs	+	−	ON	86%	14%	7	20–50
Nocodazole treatment	Absent	−	−	ON	0%	100%	37	n/a
Metaphase, Scc1 cleaved; Scc1-TEV268	Distorted	+	−	ON	39%	61%	33	50–200+

n/a: kMT length unknown because they were not within the same cryosection as the kinetochore. Note that out of 86 attached Dam1C/DASH rings, kMT-tip-to-Dam1C/DASH distances could be measured for only 56 of them. Also, note that, due to the flexibility of the rings and the low contrast of cryotomograms, not every ring could be used to make both diameter and circumference/curvature measurements.

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