Table 1. Genomic and physical DNA length... | Rockefeller University Press

Table 1.

Genomic and physical DNA length of an average HK cell, the absolute amount of Condensin subunits on mitotic chromosomes, and their linear spacing along the chromatid axis in late prometaphase, metaphase, and early anaphase

Mitotic phase/parameter	Genomic length	Physical length	Number of SMC4 (I + II)	Number of CAP-D2 (I)	Number of CAP-H (I)	Number of CAP-D3 (II)	Number of CAP-H2 (II)	Number of CAP-H (I) + CAP-H2 (II)
Prometaphase
Total	2× 7.9 Gb	1,311.7 ± 245.2 µm	247,952 ± 43,519	152,558 ± 20,965	145,478 ± 27,626	73,267 ± 14,861	36,451 ± 13,604	~181,929
Chromatid	123.4 Mb	~10.2 µm	~1,937	~1,192	~1,137	~572	~285	~1,421
1 µm	~12.0 Mb	1.0 µm	~189	~116	~111	~56	~28	~139
1 Mb	1.0 Mb	~83.0 nm	~16	~10	~9	~5	~2	~12
Genomic Condensin spacing			~63.7 kb	~103.6 kb	~108.6 kb	~215.6 kb	~433.5 kb	~86.8 kb
Physical Condensin spacing			~5.3 nm	~8.6 nm	~9.0 nm	~17.9 nm	~36.0 nm	~7.2 nm
Metaphase
Total	2× 7.9 Gb	1,162.5 ± 190.4 µm	252,652 ± 39,130	153,519 ± 22,952	140,965 ± 26,521	71,408 ± 14,999	34,543 ± 12,521	~175,509
Chromatid	123.4 Mb	~9.1 µm	~1,974	~1,199	~1,101	~558	~270	~1,371
1 µm	~13.6 Mb	1.0 µm	~217	~132	~121	~61	~30	~151
1 Mb	1.0 Mb	~73.6 nm	~16	~10	~9	~5	~2	~11
Genomic Condensin spacing			~62.5 kb	~102.9 kb	~112.1 kb	~221.3 kb	~457.4 kb	~90.0 kb
Physical Condensin spacing			~4.6 nm	~7.6 nm	~8.2 nm	~16.3 nm	~33.7 nm	~6.6 nm
Anaphase
Total	2× 7.9 Gb	923.1 ± 120.7 µm	325,522 ± 48,878	216,857 ± 32,900	194,427 ± 37,121	71,244 ± 16,174	34,677 ± 13,294	~229,104
Chromatid	123.4 Mb	~7.2 µm	~2,543	~1,694	~1,519	~557	~271	~1,790
1 µm	~17.1 Mb	1.0 µm	~353	~235	~211	~77	~38	~248
1 Mb	1.0 Mb	~58.4 nm	~21	~14	~12	~5	~2	~15
Genomic Condensin spacing			~48.5 kb	~72.9 kb	~81.3 kb	~221.8 kb	~455.6 kb	~69.0 kb
Physical Condensin spacing			~2.8 nm	~4.3 nm	~4.7 nm	~13.0 nm	~26.6 nm	~4.0 nm

Mitotic phase/parameter	Genomic length	Physical length	Number of SMC4 (I + II)	Number of CAP-D2 (I)	Number of CAP-H (I)	Number of CAP-D3 (II)	Number of CAP-H2 (II)	Number of CAP-H (I) + CAP-H2 (II)
Prometaphase
Total	2× 7.9 Gb	1,311.7 ± 245.2 µm	247,952 ± 43,519	152,558 ± 20,965	145,478 ± 27,626	73,267 ± 14,861	36,451 ± 13,604	~181,929
Chromatid	123.4 Mb	~10.2 µm	~1,937	~1,192	~1,137	~572	~285	~1,421
1 µm	~12.0 Mb	1.0 µm	~189	~116	~111	~56	~28	~139
1 Mb	1.0 Mb	~83.0 nm	~16	~10	~9	~5	~2	~12
Genomic Condensin spacing			~63.7 kb	~103.6 kb	~108.6 kb	~215.6 kb	~433.5 kb	~86.8 kb
Physical Condensin spacing			~5.3 nm	~8.6 nm	~9.0 nm	~17.9 nm	~36.0 nm	~7.2 nm
Metaphase
Total	2× 7.9 Gb	1,162.5 ± 190.4 µm	252,652 ± 39,130	153,519 ± 22,952	140,965 ± 26,521	71,408 ± 14,999	34,543 ± 12,521	~175,509
Chromatid	123.4 Mb	~9.1 µm	~1,974	~1,199	~1,101	~558	~270	~1,371
1 µm	~13.6 Mb	1.0 µm	~217	~132	~121	~61	~30	~151
1 Mb	1.0 Mb	~73.6 nm	~16	~10	~9	~5	~2	~11
Genomic Condensin spacing			~62.5 kb	~102.9 kb	~112.1 kb	~221.3 kb	~457.4 kb	~90.0 kb
Physical Condensin spacing			~4.6 nm	~7.6 nm	~8.2 nm	~16.3 nm	~33.7 nm	~6.6 nm
Anaphase
Total	2× 7.9 Gb	923.1 ± 120.7 µm	325,522 ± 48,878	216,857 ± 32,900	194,427 ± 37,121	71,244 ± 16,174	34,677 ± 13,294	~229,104
Chromatid	123.4 Mb	~7.2 µm	~2,543	~1,694	~1,519	~557	~271	~1,790
1 µm	~17.1 Mb	1.0 µm	~353	~235	~211	~77	~38	~248
1 Mb	1.0 Mb	~58.4 nm	~21	~14	~12	~5	~2	~15
Genomic Condensin spacing			~48.5 kb	~72.9 kb	~81.3 kb	~221.8 kb	~455.6 kb	~69.0 kb
Physical Condensin spacing			~2.8 nm	~4.3 nm	~4.7 nm	~13.0 nm	~26.6 nm	~4.0 nm

Calculations are based on the 7.9-billion-bp HK genome with on average 64 chromosomes (Landry et al., 2013), the whole-cell chromatid lengths for late prometaphase, metaphase, and early anaphase (Fig. 4 B), and the numbers of Condensin subunits bound to chromosomes as determined by FCS-calibrated imaging (Fig. 1, C and D). The genomic and physical Condensin spacing assumes an equidistant distribution of Condensins along the mitotic chromatid axis.

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