| Mitotic phase/parameter . | Genomic length . | Physical length . | Number of SMC4 (I + II) . | Number of CAP-D2 (I) . | Number of CAP-H (I) . | Number of CAP-D3 (II) . | Number of CAP-H2 (II) . | Number of CAP-H (I) + CAP-H2 (II) . |
|---|---|---|---|---|---|---|---|---|
| Prometaphase | ||||||||
| Total | 2× 7.9 Gb | 1,311.7 ± 245.2 µm | 247,952 ± 43,519 | 152,558 ± 20,965 | 145,478 ± 27,626 | 73,267 ± 14,861 | 36,451 ± 13,604 | ~181,929 |
| Chromatid | 123.4 Mb | ~10.2 µm | ~1,937 | ~1,192 | ~1,137 | ~572 | ~285 | ~1,421 |
| 1 µm | ~12.0 Mb | 1.0 µm | ~189 | ~116 | ~111 | ~56 | ~28 | ~139 |
| 1 Mb | 1.0 Mb | ~83.0 nm | ~16 | ~10 | ~9 | ~5 | ~2 | ~12 |
| Genomic Condensin spacing | ~63.7 kb | ~103.6 kb | ~108.6 kb | ~215.6 kb | ~433.5 kb | ~86.8 kb | ||
| Physical Condensin spacing | ~5.3 nm | ~8.6 nm | ~9.0 nm | ~17.9 nm | ~36.0 nm | ~7.2 nm | ||
| Metaphase | ||||||||
| Total | 2× 7.9 Gb | 1,162.5 ± 190.4 µm | 252,652 ± 39,130 | 153,519 ± 22,952 | 140,965 ± 26,521 | 71,408 ± 14,999 | 34,543 ± 12,521 | ~175,509 |
| Chromatid | 123.4 Mb | ~9.1 µm | ~1,974 | ~1,199 | ~1,101 | ~558 | ~270 | ~1,371 |
| 1 µm | ~13.6 Mb | 1.0 µm | ~217 | ~132 | ~121 | ~61 | ~30 | ~151 |
| 1 Mb | 1.0 Mb | ~73.6 nm | ~16 | ~10 | ~9 | ~5 | ~2 | ~11 |
| Genomic Condensin spacing | ~62.5 kb | ~102.9 kb | ~112.1 kb | ~221.3 kb | ~457.4 kb | ~90.0 kb | ||
| Physical Condensin spacing | ~4.6 nm | ~7.6 nm | ~8.2 nm | ~16.3 nm | ~33.7 nm | ~6.6 nm | ||
| Anaphase | ||||||||
| Total | 2× 7.9 Gb | 923.1 ± 120.7 µm | 325,522 ± 48,878 | 216,857 ± 32,900 | 194,427 ± 37,121 | 71,244 ± 16,174 | 34,677 ± 13,294 | ~229,104 |
| Chromatid | 123.4 Mb | ~7.2 µm | ~2,543 | ~1,694 | ~1,519 | ~557 | ~271 | ~1,790 |
| 1 µm | ~17.1 Mb | 1.0 µm | ~353 | ~235 | ~211 | ~77 | ~38 | ~248 |
| 1 Mb | 1.0 Mb | ~58.4 nm | ~21 | ~14 | ~12 | ~5 | ~2 | ~15 |
| Genomic Condensin spacing | ~48.5 kb | ~72.9 kb | ~81.3 kb | ~221.8 kb | ~455.6 kb | ~69.0 kb | ||
| Physical Condensin spacing | ~2.8 nm | ~4.3 nm | ~4.7 nm | ~13.0 nm | ~26.6 nm | ~4.0 nm |
| Mitotic phase/parameter . | Genomic length . | Physical length . | Number of SMC4 (I + II) . | Number of CAP-D2 (I) . | Number of CAP-H (I) . | Number of CAP-D3 (II) . | Number of CAP-H2 (II) . | Number of CAP-H (I) + CAP-H2 (II) . |
|---|---|---|---|---|---|---|---|---|
| Prometaphase | ||||||||
| Total | 2× 7.9 Gb | 1,311.7 ± 245.2 µm | 247,952 ± 43,519 | 152,558 ± 20,965 | 145,478 ± 27,626 | 73,267 ± 14,861 | 36,451 ± 13,604 | ~181,929 |
| Chromatid | 123.4 Mb | ~10.2 µm | ~1,937 | ~1,192 | ~1,137 | ~572 | ~285 | ~1,421 |
| 1 µm | ~12.0 Mb | 1.0 µm | ~189 | ~116 | ~111 | ~56 | ~28 | ~139 |
| 1 Mb | 1.0 Mb | ~83.0 nm | ~16 | ~10 | ~9 | ~5 | ~2 | ~12 |
| Genomic Condensin spacing | ~63.7 kb | ~103.6 kb | ~108.6 kb | ~215.6 kb | ~433.5 kb | ~86.8 kb | ||
| Physical Condensin spacing | ~5.3 nm | ~8.6 nm | ~9.0 nm | ~17.9 nm | ~36.0 nm | ~7.2 nm | ||
| Metaphase | ||||||||
| Total | 2× 7.9 Gb | 1,162.5 ± 190.4 µm | 252,652 ± 39,130 | 153,519 ± 22,952 | 140,965 ± 26,521 | 71,408 ± 14,999 | 34,543 ± 12,521 | ~175,509 |
| Chromatid | 123.4 Mb | ~9.1 µm | ~1,974 | ~1,199 | ~1,101 | ~558 | ~270 | ~1,371 |
| 1 µm | ~13.6 Mb | 1.0 µm | ~217 | ~132 | ~121 | ~61 | ~30 | ~151 |
| 1 Mb | 1.0 Mb | ~73.6 nm | ~16 | ~10 | ~9 | ~5 | ~2 | ~11 |
| Genomic Condensin spacing | ~62.5 kb | ~102.9 kb | ~112.1 kb | ~221.3 kb | ~457.4 kb | ~90.0 kb | ||
| Physical Condensin spacing | ~4.6 nm | ~7.6 nm | ~8.2 nm | ~16.3 nm | ~33.7 nm | ~6.6 nm | ||
| Anaphase | ||||||||
| Total | 2× 7.9 Gb | 923.1 ± 120.7 µm | 325,522 ± 48,878 | 216,857 ± 32,900 | 194,427 ± 37,121 | 71,244 ± 16,174 | 34,677 ± 13,294 | ~229,104 |
| Chromatid | 123.4 Mb | ~7.2 µm | ~2,543 | ~1,694 | ~1,519 | ~557 | ~271 | ~1,790 |
| 1 µm | ~17.1 Mb | 1.0 µm | ~353 | ~235 | ~211 | ~77 | ~38 | ~248 |
| 1 Mb | 1.0 Mb | ~58.4 nm | ~21 | ~14 | ~12 | ~5 | ~2 | ~15 |
| Genomic Condensin spacing | ~48.5 kb | ~72.9 kb | ~81.3 kb | ~221.8 kb | ~455.6 kb | ~69.0 kb | ||
| Physical Condensin spacing | ~2.8 nm | ~4.3 nm | ~4.7 nm | ~13.0 nm | ~26.6 nm | ~4.0 nm |
Calculations are based on the 7.9-billion-bp HK genome with on average 64 chromosomes (Landry et al., 2013), the whole-cell chromatid lengths for late prometaphase, metaphase, and early anaphase (Fig. 4 B), and the numbers of Condensin subunits bound to chromosomes as determined by FCS-calibrated imaging (Fig. 1, C and D). The genomic and physical Condensin spacing assumes an equidistant distribution of Condensins along the mitotic chromatid axis.