Table 1. Summary of motility behavior... | Rockefeller University Press

Table 1.

Summary of motility behavior and kinetics of kinesin-4 motors

Family	Motor	Single molecule assay (velocity)	Multimotor assay		*k_cat*	K_0.5_,MT	*K_m,ATP*
Family	Motor	Single molecule assay (velocity)	Microtubule gliding assay (velocity)	Cellular transport ability	*k_cat*	K_0.5_,MT	*K_m,ATP*
		nm/s	nm/s		s⁻¹site⁻¹	µM	µM
Kinesin-1	KIF5C^{^a}	664 ± 10	728.3 ± 6.4	Fast transport^{^b}	20 ± 2^{^c}	0.3 ± 0.1^{^c}	23 ± 9^{^c}
Kinesin-4
KIF7	MmKIF27	13.51 ± 0.17	10.8 ± 0.4	Slow transport	2.5	0.04	0.6
	MmKIF7	0	0	No transport	0.02	NA	NA
	DmCos2	0	ND	ND	ND	ND	ND
KIF21	MmKIF21A	391.3 ± 4.7	224.2 ± 4.3	Fast transport	ND	ND	ND
	DmKLP31E	375.3 ± 5.2	ND	ND	ND	ND	ND
	CeKLP12	623 ± 13	ND	ND	ND	ND	ND
KIF4	MmKIF4	908 ± 15	836 ± 13	Fast transport	ND	ND	ND

Family	Motor	Single molecule assay (velocity)	Multimotor assay		k_cat	K_0.5_,MT	K_m,ATP
Family	Motor	Single molecule assay (velocity)	Microtubule gliding assay (velocity)	Cellular transport ability	k_cat	K_0.5_,MT	K_m,ATP
		nm/s	nm/s		s⁻¹site⁻¹	µM	µM
Kinesin-1	KIF5C^a	664 ± 10	728.3 ± 6.4	Fast transport^b	20 ± 2^c	0.3 ± 0.1^c	23 ± 9^c
Kinesin-4
KIF7	MmKIF27	13.51 ± 0.17	10.8 ± 0.4	Slow transport	2.5	0.04	0.6
	MmKIF7	0	0	No transport	0.02	NA	NA
	DmCos2	0	ND	ND	ND	ND	ND
KIF21	MmKIF21A	391.3 ± 4.7	224.2 ± 4.3	Fast transport	ND	ND	ND
	DmKLP31E	375.3 ± 5.2	ND	ND	ND	ND	ND
	CeKLP12	623 ± 13	ND	ND	ND	ND	ND
KIF4	MmKIF4	908 ± 15	836 ± 13	Fast transport	ND	ND	ND

NA, not applicable (could not be determined due to low k_cat); ND, not determined.

For kinesin-1, the data in the table state motility behavior of RnKIF5C from this study.

Engelke et al. (2016).

Kinetics of HsKIF5C from Cochran (2015).

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