Table 1. Speed and frequency of... | Rockefeller University Press

Table 1.

Speed and frequency of anterograde IFT trafficking

	Speed (µm/s)	Frequency (trains/s)	n trains
mNG::IFT81
Left	2.43 ± 0.68	0.52 ± 0.22	284
Right	2.27 ± 0.65	0.45 ± 0.18	245
Left + right	2.35 ± 0.62	0.98 ± 0.33	529
Unresolved^{^a}	2.60 ± 0.46	0.76 ± 0.11	389
GFP::IFT52
Left	2.56 ± 0.26	0.43 ± 0.10	138
Right	2.18 ± 0.29	0.34 ± 0.17	110
Left + right	2.37 ± 0.26	0.77 ± 0.15	248

	Speed (µm/s)	Frequency (trains/s)	n trains
mNG::IFT81
Left	2.43 ± 0.68	0.52 ± 0.22	284
Right	2.27 ± 0.65	0.45 ± 0.18	245
Left + right	2.35 ± 0.62	0.98 ± 0.33	529
Unresolved^{^a}	2.60 ± 0.46	0.76 ± 0.11	389
GFP::IFT52
Left	2.56 ± 0.26	0.43 ± 0.10	138
Right	2.18 ± 0.29	0.34 ± 0.17	110
Left + right	2.37 ± 0.26	0.77 ± 0.15	248

Data were obtained from 18 and 10 cells for mNG::IFT81 and GFP::IFT52, respectively.

Corresponds with videos acquired in high-resolution conditions but where only one track could be visualized, presumably due to the orientation of the flagellum during the acquisition. Indeed, doublets 3–4 and 7–8 are on opposing sides of the axoneme. According to the orientation of the flagellum on the slide, they can be parallel to the glass slide, and two tracks are visible. However, if the flagellum is twisted by 90°, the two sets of doublets will be on top of each other and will not be resolved even when using high-resolution conditions.

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