Table 2. Curve fitting of FRAP data... | Rockefeller University Press

Table 2.

Curve fitting of FRAP data

	GFP-actin	F(0)	F(e)	c	τ1 (sec)	τ2 (sec)
Control	WT	0.0960	0.8797	0.4161	1.5112	44.0287
	G13R	0.1041	1.0019	0.4637	1.4744	69.3191
	R62D	0.1021	1.0002	0.4506	1.5724	70.2468
DMSO	G13R	0.10126	1.0006	0.45883	1.5190	69.4147
	R62D	0.098472	1.0035	0.45535	1.5174	72.7302
LY294002	G13R	0.1274	1.0004	0.34306↓	1.3793	69.8583
	R62D	0.16293	0.99987	0.3464↓	1.4974	68.949
SF1670	G13R	0.087312	0.9997	0.57153↑	1.6203	70.3258
	R62D	0.081741	1.0016	0.57138↑	1.5003	70.0223
Scramble RNAi	G13R	0.099384	0.99966	0.45277	1.5389	70.3558
	R62D	0.098478	1.0030	0.45301	1.5687	72.6469
Profilin 1/2 RNAi	G13R	0.16664	0.9999	0.17228↓	1.5483	72.8719
	R62D	0.16208	1.0016	0.17482↓	1.5657	74.7082

	GFP-actin	F(0)	F(e)	c	τ1 (sec)	τ2 (sec)
Control	WT	0.0960	0.8797	0.4161	1.5112	44.0287
	G13R	0.1041	1.0019	0.4637	1.4744	69.3191
	R62D	0.1021	1.0002	0.4506	1.5724	70.2468
DMSO	G13R	0.10126	1.0006	0.45883	1.5190	69.4147
	R62D	0.098472	1.0035	0.45535	1.5174	72.7302
LY294002	G13R	0.1274	1.0004	0.34306↓	1.3793	69.8583
	R62D	0.16293	0.99987	0.3464↓	1.4974	68.949
SF1670	G13R	0.087312	0.9997	0.57153↑	1.6203	70.3258
	R62D	0.081741	1.0016	0.57138↑	1.5003	70.0223
Scramble RNAi	G13R	0.099384	0.99966	0.45277	1.5389	70.3558
	R62D	0.098478	1.0030	0.45301	1.5687	72.6469
Profilin 1/2 RNAi	G13R	0.16664	0.9999	0.17228↓	1.5483	72.8719
	R62D	0.16208	1.0016	0.17482↓	1.5657	74.7082

The comprehensive table summarizes all five parameters (F[0], F[e], c, τ1, and τ2) of the double exponential function used to fit the FRAP curves of WT actin or actin mutants. Note that τ1 and τ2 are similar among actin mutants under different conditions. Arrows indicate the changes with bold numbers depicting an increase over the control.

[ViewLarge]

Sharing Unavailable