| . | GFP-actin . | F(0) . | F(e) . | c . | τ1 (sec) . | τ2 (sec) . |
|---|---|---|---|---|---|---|
| Control | WT | 0.0960 | 0.8797 | 0.4161 | 1.5112 | 44.0287 |
| G13R | 0.1041 | 1.0019 | 0.4637 | 1.4744 | 69.3191 | |
| R62D | 0.1021 | 1.0002 | 0.4506 | 1.5724 | 70.2468 | |
| DMSO | G13R | 0.10126 | 1.0006 | 0.45883 | 1.5190 | 69.4147 |
| R62D | 0.098472 | 1.0035 | 0.45535 | 1.5174 | 72.7302 | |
| LY294002 | G13R | 0.1274 | 1.0004 | 0.34306↓ | 1.3793 | 69.8583 |
| R62D | 0.16293 | 0.99987 | 0.3464↓ | 1.4974 | 68.949 | |
| SF1670 | G13R | 0.087312 | 0.9997 | 0.57153↑ | 1.6203 | 70.3258 |
| R62D | 0.081741 | 1.0016 | 0.57138↑ | 1.5003 | 70.0223 | |
| Scramble RNAi | G13R | 0.099384 | 0.99966 | 0.45277 | 1.5389 | 70.3558 |
| R62D | 0.098478 | 1.0030 | 0.45301 | 1.5687 | 72.6469 | |
| Profilin 1/2 RNAi | G13R | 0.16664 | 0.9999 | 0.17228↓ | 1.5483 | 72.8719 |
| R62D | 0.16208 | 1.0016 | 0.17482↓ | 1.5657 | 74.7082 |
| . | GFP-actin . | F(0) . | F(e) . | c . | τ1 (sec) . | τ2 (sec) . |
|---|---|---|---|---|---|---|
| Control | WT | 0.0960 | 0.8797 | 0.4161 | 1.5112 | 44.0287 |
| G13R | 0.1041 | 1.0019 | 0.4637 | 1.4744 | 69.3191 | |
| R62D | 0.1021 | 1.0002 | 0.4506 | 1.5724 | 70.2468 | |
| DMSO | G13R | 0.10126 | 1.0006 | 0.45883 | 1.5190 | 69.4147 |
| R62D | 0.098472 | 1.0035 | 0.45535 | 1.5174 | 72.7302 | |
| LY294002 | G13R | 0.1274 | 1.0004 | 0.34306↓ | 1.3793 | 69.8583 |
| R62D | 0.16293 | 0.99987 | 0.3464↓ | 1.4974 | 68.949 | |
| SF1670 | G13R | 0.087312 | 0.9997 | 0.57153↑ | 1.6203 | 70.3258 |
| R62D | 0.081741 | 1.0016 | 0.57138↑ | 1.5003 | 70.0223 | |
| Scramble RNAi | G13R | 0.099384 | 0.99966 | 0.45277 | 1.5389 | 70.3558 |
| R62D | 0.098478 | 1.0030 | 0.45301 | 1.5687 | 72.6469 | |
| Profilin 1/2 RNAi | G13R | 0.16664 | 0.9999 | 0.17228↓ | 1.5483 | 72.8719 |
| R62D | 0.16208 | 1.0016 | 0.17482↓ | 1.5657 | 74.7082 |
The comprehensive table summarizes all five parameters (F[0], F[e], c, τ1, and τ2) of the double exponential function used to fit the FRAP curves of WT actin or actin mutants. Note that τ1 and τ2 are similar among actin mutants under different conditions. Arrows indicate the changes with bold numbers depicting an increase over the control.