| . | GFP-actin . | 50% Prebleaching . | 50% plateau . | 90% plateau . | 99% plateau . | Plateau at 300 s (%) . |
|---|---|---|---|---|---|---|
| DMSO | G13R | 3.1 ± 0.3 | 3.0 ± 0.3 | 100 ± 5.5 | 249 ± 16 | 99 ± 0.62 |
| R62D | 3.0 ± 0.4 | 3.0 ± 0.3 | 101 ± 5.8 | 248 ± 15 | 99 ± 0.84 | |
| LY294002 | G13R | 1.7 ± 0.3 | 1.7 ± 0.2 | 85 ± 4.3 | 221 ± 13 | 99 ± 0.82 |
| R62D | 1.8 ± 0.2 | 1.8 ± 0.2 | 84 ± 4.7 | 223 ± 14 | 99 ± 0.99 | |
| SF1670 | G13R | 10.0 ± 0.3 | 9.4 ± 0.3 | 116 ± 6.9 | 274 ± 16 | 99 ± 0.51 |
| R62D | 9.4 ± 0.4 | 9.2 ± 0.3 | 115 ± 7.2 | 272 ± 17 | 99 ± 0.56 | |
| Scramble RNAi | G13R | 3.1 ± 0.3 | 3.1 ± 0.2 | 99 ± 6.0 | 253 ± 16 | 99 ± 0.53 |
| R62D | 3.1 ± 0.2 | 3.1 ± 0.2 | 100 ± 5.7 | 260 ± 15 | 99 ± 0.56 | |
| Profilin 1/2 RNAi | G13R | 1.2 ± 0.3 | 1.1 ± 0.3 | 40 ± 4.2 | 173 ± 14 | 99 ± 0.95 |
| R62D | 1.2 ± 0.3 | 1.2 ± 0.2 | 38 ± 4.5 | 169 ± 13 | 99 ± 1.00 |
| . | GFP-actin . | 50% Prebleaching . | 50% plateau . | 90% plateau . | 99% plateau . | Plateau at 300 s (%) . |
|---|---|---|---|---|---|---|
| DMSO | G13R | 3.1 ± 0.3 | 3.0 ± 0.3 | 100 ± 5.5 | 249 ± 16 | 99 ± 0.62 |
| R62D | 3.0 ± 0.4 | 3.0 ± 0.3 | 101 ± 5.8 | 248 ± 15 | 99 ± 0.84 | |
| LY294002 | G13R | 1.7 ± 0.3 | 1.7 ± 0.2 | 85 ± 4.3 | 221 ± 13 | 99 ± 0.82 |
| R62D | 1.8 ± 0.2 | 1.8 ± 0.2 | 84 ± 4.7 | 223 ± 14 | 99 ± 0.99 | |
| SF1670 | G13R | 10.0 ± 0.3 | 9.4 ± 0.3 | 116 ± 6.9 | 274 ± 16 | 99 ± 0.51 |
| R62D | 9.4 ± 0.4 | 9.2 ± 0.3 | 115 ± 7.2 | 272 ± 17 | 99 ± 0.56 | |
| Scramble RNAi | G13R | 3.1 ± 0.3 | 3.1 ± 0.2 | 99 ± 6.0 | 253 ± 16 | 99 ± 0.53 |
| R62D | 3.1 ± 0.2 | 3.1 ± 0.2 | 100 ± 5.7 | 260 ± 15 | 99 ± 0.56 | |
| Profilin 1/2 RNAi | G13R | 1.2 ± 0.3 | 1.1 ± 0.3 | 40 ± 4.2 | 173 ± 14 | 99 ± 0.95 |
| R62D | 1.2 ± 0.3 | 1.2 ± 0.2 | 38 ± 4.5 | 169 ± 13 | 99 ± 1.00 |
Quantification table showing recovery time for 50% prebleaching levels, 50% plateau levels, 90% and 99% plateau levels, and the final recovery percentages of EGFP-R62D/G13R in dendritic spines under different conditions. All numbers are presented in seconds as mean ± SEM.