Table 1.
Quantification of mitotic cells after colchicine treatment
WTmad2ΔaldG4422
pH3-positive cells per intestinal region Sucrose 0.29 ± 0.85 3.11 ± 3.90 5.73 ± 4.82 
Colchicine 34.81 ± 30.46**** 12.62 ± 12.60 34.27 ± 19.74 
Mitotic index fold change (sucrose vs. colchicine)  ×120 ×4 ×6 
WTmad2ΔaldG4422
pH3-positive cells per intestinal region Sucrose 0.29 ± 0.85 3.11 ± 3.90 5.73 ± 4.82 
Colchicine 34.81 ± 30.46**** 12.62 ± 12.60 34.27 ± 19.74 
Mitotic index fold change (sucrose vs. colchicine)  ×120 ×4 ×6 

Quantification of pH3-positive cells present in the first two fields of view of the posterior midgut after the pyloric ring (40× objective) of WT and homozygous mutants for mad2 or mps1. n (intestines) = 24 for WT on sucrose; n (intestines) = 47 for WT on sucrose + colchicine; n (intestines) = 22 for mad2 mutants on sucrose; n (intestines) = 29 for mad2 mutants on sucrose + colchicine; n (intestines) = 11 for mps1 mutants on sucrose; n (intestines) = 11 for mps1 mutants on sucrose + colchicine. ****, P < 0.0001; Mann–Whitney U test.

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