| Vector and insert . | Tag . | Remarks . |
|---|---|---|
| pEGFP-C2 | ||
| Full-length, truncated, and mutated human lyspersin (C17orf59) | N-EGFP | This study |
| pcDNA3.1 | ||
| Human myrlysin | N-OSF; C-FOS | Pu et al., 2015 |
| Human BLOS2 | N-OSF | Pu et al., 2015 |
| Human lyspersin | N-OSF; C-FOS | Pu et al., 2015 |
| Human pallidin | N-OSF | This study |
| Human LAMTOR1 | C-FOS | This study |
| pCI-neo | ||
| Human lyspersin | N-3myc | This study |
| p-mCherry-N1 | ||
| Human Arl8b | C-mCherry | This study |
| pLKO.5-puro | ||
| Nontargeting shRNA | Sigma-Aldrich (SHC216) | |
| Human LAMTOR1 shRNA | Sigma-Aldrich (SHCLNG-NM_017907, TRCN0000282725) | |
| pLKO.1 | ||
| Human RAPTOR shRNA | Addgene (1858) | |
| pSpCas9(BB)-2A-GFP (PX458) | ||
| Sequence from human LAMTOR2 | EGFP, FLAG | Addgene (48138) |
| pQCXIP | ||
| Human SLC38A9 | N-EGFP | This study |
| pGBT9 | ||
| Human lyspersin | This study | |
| Human snapin | This study | |
| Human MEF2BNB | This study | |
| Human myrlysin | This study | |
| Human BLOS1 | This study | |
| Human BLOS2 | This study | |
| Human KXD1 | This study | |
| Human diaskedin | This study | |
| pGADT7 | ||
| Human lyspersin | This study | |
| Human snapin | This study | |
| Human MEF2BNB | This study | |
| Human myrlysin | This study | |
| Human BLOS1 | This study | |
| Human BLOS2 | This study | |
| Human KXD1 | This study | |
| Human diaskedin | This study | |
| Human LAMTOR1 | This study | |
| Human LAMTOR2 and mutants | This study | |
| Human LAMTOR3 | This study | |
| Human LAMTOR4 | This study | |
| Human LAMTOR5 | This study | |
| pGBT9 | ||
| Human lyspersin 190-255 | This study | |
| Human lyspersin 256-357 | This study | |
| Human lyspersin 190-357 | This study | |
| Human lyspersin 190-end F216A | This study | |
| Human lyspersin 190-end L221A | This study | |
| Human lyspersin 190-end L349E, L352E | This study | |
| Human lyspersin 256-357 | This study | |
| Human lyspersin 209-357 | This study |
| Vector and insert . | Tag . | Remarks . |
|---|---|---|
| pEGFP-C2 | ||
| Full-length, truncated, and mutated human lyspersin (C17orf59) | N-EGFP | This study |
| pcDNA3.1 | ||
| Human myrlysin | N-OSF; C-FOS | Pu et al., 2015 |
| Human BLOS2 | N-OSF | Pu et al., 2015 |
| Human lyspersin | N-OSF; C-FOS | Pu et al., 2015 |
| Human pallidin | N-OSF | This study |
| Human LAMTOR1 | C-FOS | This study |
| pCI-neo | ||
| Human lyspersin | N-3myc | This study |
| p-mCherry-N1 | ||
| Human Arl8b | C-mCherry | This study |
| pLKO.5-puro | ||
| Nontargeting shRNA | Sigma-Aldrich (SHC216) | |
| Human LAMTOR1 shRNA | Sigma-Aldrich (SHCLNG-NM_017907, TRCN0000282725) | |
| pLKO.1 | ||
| Human RAPTOR shRNA | Addgene (1858) | |
| pSpCas9(BB)-2A-GFP (PX458) | ||
| Sequence from human LAMTOR2 | EGFP, FLAG | Addgene (48138) |
| pQCXIP | ||
| Human SLC38A9 | N-EGFP | This study |
| pGBT9 | ||
| Human lyspersin | This study | |
| Human snapin | This study | |
| Human MEF2BNB | This study | |
| Human myrlysin | This study | |
| Human BLOS1 | This study | |
| Human BLOS2 | This study | |
| Human KXD1 | This study | |
| Human diaskedin | This study | |
| pGADT7 | ||
| Human lyspersin | This study | |
| Human snapin | This study | |
| Human MEF2BNB | This study | |
| Human myrlysin | This study | |
| Human BLOS1 | This study | |
| Human BLOS2 | This study | |
| Human KXD1 | This study | |
| Human diaskedin | This study | |
| Human LAMTOR1 | This study | |
| Human LAMTOR2 and mutants | This study | |
| Human LAMTOR3 | This study | |
| Human LAMTOR4 | This study | |
| Human LAMTOR5 | This study | |
| pGBT9 | ||
| Human lyspersin 190-255 | This study | |
| Human lyspersin 256-357 | This study | |
| Human lyspersin 190-357 | This study | |
| Human lyspersin 190-end F216A | This study | |
| Human lyspersin 190-end L221A | This study | |
| Human lyspersin 190-end L349E, L352E | This study | |
| Human lyspersin 256-357 | This study | |
| Human lyspersin 209-357 | This study |