Table 1. Lesion and amino acid sequences... | Rockefeller University Press

Table 1.

Lesion and amino acid sequences used to generate the MyD88^CR2.8 allele

Allele	Sequence (5′–3′)
Lesion
MyD88^WT	GTCAGTTATCGGCGTTATCGCACCGCTGGCATGGTGGTGGCCGAGGGAGTTATGGACTCC
MyD88^CR2.8	GTCAGTTATCGGCGTTATCGCACCGCTGGCATGGTGGTGGCCGAGGGAGTTATG
MyD88^WT	GGGTCGGGATCGGGCACGGGAACGGGCTTGGGGCACTTCAACGAGACCCCATTATCCGCA
MyD88^CR2.8	GGTCGGGATCGGGCACGGGAACGGGCTTGGGGCACTTCAACGAGACCCCATTATCCGCA
Amino acid
MyD88^WT	MRPRFVCHQQHSVAHSHYQPHSHFHHHTHRHPNPPHHHHIYGATDVSYRRYRTAGMVVAE
MyD88^CR2.8	MRPRFVCHQQHSVAHSHYQPHSHFHHHTHRHPNPPHHHHIYGATDVSYRRYRTAGMVVAE
MyD88^WT	GVMDSGSGSGTGTGL------GHFNETPLSALGIETRTQLSRMLNRKKVLRSEEGYQRDW
MyD88^CR2.8	GVMGRDRARERAWGTSTRPHYPHWASRPAPSCPACSTOP

Allele	Sequence (5′–3′)
Lesion
MyD88^WT	GTCAGTTATCGGCGTTATCGCACCGCTGGCATGGTGGTGGCCGAGGGAGTTATGGACTCC
MyD88^CR2.8	GTCAGTTATCGGCGTTATCGCACCGCTGGCATGGTGGTGGCCGAGGGAGTTATG
MyD88^WT	GGGTCGGGATCGGGCACGGGAACGGGCTTGGGGCACTTCAACGAGACCCCATTATCCGCA
MyD88^CR2.8	GGTCGGGATCGGGCACGGGAACGGGCTTGGGGCACTTCAACGAGACCCCATTATCCGCA
Amino acid
MyD88^WT	MRPRFVCHQQHSVAHSHYQPHSHFHHHTHRHPNPPHHHHIYGATDVSYRRYRTAGMVVAE
MyD88^CR2.8	MRPRFVCHQQHSVAHSHYQPHSHFHHHTHRHPNPPHHHHIYGATDVSYRRYRTAGMVVAE
MyD88^WT	GVMDSGSGSGTGTGL------GHFNETPLSALGIETRTQLSRMLNRKKVLRSEEGYQRDW
MyD88^CR2.8	GVMGRDRARERAWGTSTRPHYPHWASRPAPSCPACSTOP

Guide RNA sequences are given in bold.