Table 1.
PLEKHA7 IP and proteomics reveal association with the RISC
Gene IDGene nameExclusive unique spectrum countProtein identification probability
Adherens junctions controls    
  CDH1 Cadherin-1; E-cadherin 100.00% 
  CTNND1 Catenin δ-1; p120 catenin 100.00% 
RISC-related hits    
Core RISC proteins    
  EIF2C2 Protein Argonaute-2 Ago2 18 100.00% 
  PABPC1 Polyadenylate-binding protein 1 20 100.00% 
  TNRC6A Trinucleotide repeat-containing gene 6A protein (GW182) 100.00% 
  TNRC6B Trinucleotide repeat-containing gene 6B 16 100.00% 
Accessory proteins    
  DDX5 Probable ATP-dependent RNA helicase DDX5 100.00% 
  DDX6 Probable ATP-dependent RNA helicase DDX6 100.00% 
  DDX17 Probable ATP-dependent RNA helicase DDX17 20 100.00% 
  DDX20 Probable ATP-dependent RNA helicase DDX20 14 100.00% 
  ELAVL1 ELAV-like protein 1 100.00% 
  GEMIN4 Gem-associated protein 4 100.00% 
  HNRNPA2B1 Heterogeneous nuclear ribonucleoprotein A2/B1 31 100.00% 
  HNRNPC Heterogeneous nuclear ribonucleoprotein C1/C2 100.00% 
  HNRNPF Heterogeneous nuclear ribonucleoprotein F 17 100.00% 
  HNRNPH3 Heterogeneous nuclear ribonucleoprotein H3 100.00% 
  HNRNPM Heterogeneous nuclear ribonucleoprotein M 21 100.00% 
  HSP90AB1 HSP 90-β 21 100.00% 
  HSP90AA1 HSP 90-α 10 100.00% 
  HSPA1A Heat shock 70-kD protein 1A/1B 14 100.00% 
  HSPA8 Heat shock cognate, 71 kD 40 100.00% 
  IGF2BP1 Insulin-like growth factor 2 mRNA-binding protein 1 28 100.00% 
  IGF2BP2 Insulin-like growth factor 2 mRNA-binding protein 2 100.00% 
  IGF2BP3 Insulin-like growth factor 2 mRNA-binding protein 3 10 100.00% 
  MOV10 Putative helicase MOV-10 12 100.00% 
  RALY RNA-binding protein Raly 100.00% 
  RBM4 RNA-binding protein 4 12 100.00% 
  YBX1 Nuclease-sensitive element-binding protein 1 100.00% 
miRLC-related hits    
  DICER Isoform 2 of endoribonuclease Dicer 100.00% 
  TRBP Isoform 2 of TAR DNA-binding protein 12 100.00% 
Gene IDGene nameExclusive unique spectrum countProtein identification probability
Adherens junctions controls    
  CDH1 Cadherin-1; E-cadherin 100.00% 
  CTNND1 Catenin δ-1; p120 catenin 100.00% 
RISC-related hits    
Core RISC proteins    
  EIF2C2 Protein Argonaute-2 Ago2 18 100.00% 
  PABPC1 Polyadenylate-binding protein 1 20 100.00% 
  TNRC6A Trinucleotide repeat-containing gene 6A protein (GW182) 100.00% 
  TNRC6B Trinucleotide repeat-containing gene 6B 16 100.00% 
Accessory proteins    
  DDX5 Probable ATP-dependent RNA helicase DDX5 100.00% 
  DDX6 Probable ATP-dependent RNA helicase DDX6 100.00% 
  DDX17 Probable ATP-dependent RNA helicase DDX17 20 100.00% 
  DDX20 Probable ATP-dependent RNA helicase DDX20 14 100.00% 
  ELAVL1 ELAV-like protein 1 100.00% 
  GEMIN4 Gem-associated protein 4 100.00% 
  HNRNPA2B1 Heterogeneous nuclear ribonucleoprotein A2/B1 31 100.00% 
  HNRNPC Heterogeneous nuclear ribonucleoprotein C1/C2 100.00% 
  HNRNPF Heterogeneous nuclear ribonucleoprotein F 17 100.00% 
  HNRNPH3 Heterogeneous nuclear ribonucleoprotein H3 100.00% 
  HNRNPM Heterogeneous nuclear ribonucleoprotein M 21 100.00% 
  HSP90AB1 HSP 90-β 21 100.00% 
  HSP90AA1 HSP 90-α 10 100.00% 
  HSPA1A Heat shock 70-kD protein 1A/1B 14 100.00% 
  HSPA8 Heat shock cognate, 71 kD 40 100.00% 
  IGF2BP1 Insulin-like growth factor 2 mRNA-binding protein 1 28 100.00% 
  IGF2BP2 Insulin-like growth factor 2 mRNA-binding protein 2 100.00% 
  IGF2BP3 Insulin-like growth factor 2 mRNA-binding protein 3 10 100.00% 
  MOV10 Putative helicase MOV-10 12 100.00% 
  RALY RNA-binding protein Raly 100.00% 
  RBM4 RNA-binding protein 4 12 100.00% 
  YBX1 Nuclease-sensitive element-binding protein 1 100.00% 
miRLC-related hits    
  DICER Isoform 2 of endoribonuclease Dicer 100.00% 
  TRBP Isoform 2 of TAR DNA-binding protein 12 100.00% 

Hits are in alphabetical order for each category. HSP, heat shock protein.

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