| Name . | Description . | Source . | Used in figure . |
|---|---|---|---|
| pNVT-ST-149–238 | 6His-Ste5-RING-H2149–238 | Walczak et al., 2014 | Fig. 2, B–D and F; Fig. 4, D and E; and Fig. S4 |
| pNVT-ST-149–238 S185A | 6His-Ste5-RING-H2149–238 S185A | This study | Figs. 2 E, 4 E, and S4 |
| pNVT-ST-149–238 S185D | 6His-Ste5-RING-H2149–238 S185D | This study | Fig. 4, D and E |
| pNVT-FR-RING-H2 | 6His-Far1-RING-H2173-261 | This study | Fig. 2 B |
| pMG270 | pRD53 GAL1 PKCR398A | Collection | Fig. 5 B and Fig. S2, D–F |
| pMG269 | pRD53 GAL1 PKCK853R | Collection | Fig. 1 C |
| pGADXP | 2 µm LEU2 ADH1pr GAL4 AD | Lab collection | Fig. S5, A and B |
| pBH13 | lexA DBD HA-Far1174–285 | This study | Fig. S5, A and B |
| pBH14 | GAL4 AD HA-Ste4 | Fig. S5, A and B | |
| pBH65 | lexA DBD HA-Far1174–285 3A S208S210S211 | This study | Fig. S5, A and B |
| pBH67 | lexA DBD HA-Far1174–285 3D S208S210S211 | This study | Fig. S5, A and B |
| pSP151 | pRS316 endo-Ste5S185A-3xGFP | This study | Fig. 4, A and B; and Fig. 6, F and G |
| pSP167 | pRS303 pGAL1-Ste5S185D-TMD | This study | Fig. 4 C |
| pSP169 | pRS303 pGAL1-Ste5S185A-TMD | This study | Fig. 4 C |
| pSP174 | pRS316 endo-Ste5S185D-3xGFP | This study | Fig. 4, A and B |
| pSP175 | pRS316 endo-Ste5-3xGFP | This study | Fig. 4, A and B; and Fig. 6, E–G |
| pSP186 | pRS303 endo-Ste5-13myc | This study | Fig. 4 C |
| pSP188 | pRS303 endo-Ste5S185D-13myc | This study | Fig. 4 C |
| pSP193 | Msn2p-NLS-GFP (pPKI-NESMsn2p(567–704)-GFP) LEU2 | Görner et al., 2002 | Fig. S1 D |
| pFD344 | pRS303 pGAL1-Ste5-TMD | This study | Fig. 4 C |
| pFD532 | GAL1-GST-Pkc1 URA3 (YBL105C) | Collection | Fig. 2 B |
| pFD720 | pRS426 pGAL1-GST-Ste18–Ste4 | This study | Fig. 4, D and E |
| Name . | Description . | Source . | Used in figure . |
|---|---|---|---|
| pNVT-ST-149–238 | 6His-Ste5-RING-H2149–238 | Walczak et al., 2014 | Fig. 2, B–D and F; Fig. 4, D and E; and Fig. S4 |
| pNVT-ST-149–238 S185A | 6His-Ste5-RING-H2149–238 S185A | This study | Figs. 2 E, 4 E, and S4 |
| pNVT-ST-149–238 S185D | 6His-Ste5-RING-H2149–238 S185D | This study | Fig. 4, D and E |
| pNVT-FR-RING-H2 | 6His-Far1-RING-H2173-261 | This study | Fig. 2 B |
| pMG270 | pRD53 GAL1 PKCR398A | Collection | Fig. 5 B and Fig. S2, D–F |
| pMG269 | pRD53 GAL1 PKCK853R | Collection | Fig. 1 C |
| pGADXP | 2 µm LEU2 ADH1pr GAL4 AD | Lab collection | Fig. S5, A and B |
| pBH13 | lexA DBD HA-Far1174–285 | This study | Fig. S5, A and B |
| pBH14 | GAL4 AD HA-Ste4 | Fig. S5, A and B | |
| pBH65 | lexA DBD HA-Far1174–285 3A S208S210S211 | This study | Fig. S5, A and B |
| pBH67 | lexA DBD HA-Far1174–285 3D S208S210S211 | This study | Fig. S5, A and B |
| pSP151 | pRS316 endo-Ste5S185A-3xGFP | This study | Fig. 4, A and B; and Fig. 6, F and G |
| pSP167 | pRS303 pGAL1-Ste5S185D-TMD | This study | Fig. 4 C |
| pSP169 | pRS303 pGAL1-Ste5S185A-TMD | This study | Fig. 4 C |
| pSP174 | pRS316 endo-Ste5S185D-3xGFP | This study | Fig. 4, A and B |
| pSP175 | pRS316 endo-Ste5-3xGFP | This study | Fig. 4, A and B; and Fig. 6, E–G |
| pSP186 | pRS303 endo-Ste5-13myc | This study | Fig. 4 C |
| pSP188 | pRS303 endo-Ste5S185D-13myc | This study | Fig. 4 C |
| pSP193 | Msn2p-NLS-GFP (pPKI-NESMsn2p(567–704)-GFP) LEU2 | Görner et al., 2002 | Fig. S1 D |
| pFD344 | pRS303 pGAL1-Ste5-TMD | This study | Fig. 4 C |
| pFD532 | GAL1-GST-Pkc1 URA3 (YBL105C) | Collection | Fig. 2 B |
| pFD720 | pRS426 pGAL1-GST-Ste18–Ste4 | This study | Fig. 4, D and E |