Table 1.
Yeast strains
NameGenotypeSourceUsed in figure
BY4741 MATa his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 OpenBiosystems Parental 
w303 leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 Lab collection Parental 
yRM119 BY4741 Prps2-Ste7DS(1-30)-2xNLS(9SP)-Cherry::URA3 hta2Δ::Hta2-CFP::HIS3 Durandau et al., 2015  Fig. 1, D and E; and Fig. S1 A 
yRM120 BY4741 pkc1Δ::PKC1-GFP-HIS3MX6 ura3Δ::TMD-dCherry Mishra et al., 2017  Fig. S2 A 
yRM230 BY4741 Bni1-qV::URA3 TMD-dCherry::LEU2 fus3Δ::Fus3-as1::HIS3 Mishra et al., 2017  Fig. 1 F  
yRM242 w303 ste5Δ::ADE2, STE5-tV::LEU2 This study Fig. 1, A–C, G, and H; Fig. 5, C and D; and Fig. S2, C–F 
yRM243 w303 ste5Δ::ADE2, ste5Δ:: Ste5S185A-tV::LEU2 This study Fig. 5, C and D  
yRM244 yRM242 mpk1Δ::kanMX This study Fig. 1, G and H; and Fig. S2 C 
yRM251 yRM242 empty - TRP1 This study Fig. S2 G 
yRM252 yRM242 BCK1-20::TRP1 This study Fig. S2 G 
yRM253 BY4741 gat1Δ::GAT1-GFP-HIS3MX6 Collection Fig. S1 B 
yRM254 BY4741 sfp1Δ::SFP1-GFP-HIS3MX6 Collection Fig. S1 C 
WR232 w303 SILAC STE5-HTBn::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study, W303 SILAC background described in Reiter et al., 2012  Figs. 2 A and S3 
WR254 w303 SILAC FAR1-HTBeaq::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR484 w303 SILAC STE5-HTBn::hphMX, FAR1-HTBeaq::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR658 w303 SILAC Ste5HTBnp::hphMX, ste7Δ::HIS3, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR725 w303 Ste5-HTBn::hphMX, ste7Δ::HIS3, ste12Δ::kanMX, sst2Δ::natMX This study Figs. 2 A and S3 
WR800 w303 SILAC STE5-HTB::hphMX, stl2Δ::kanMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
JA596 w303 STE5-HKMT-myc::LEU2; PKC1-protA-H3-HA::URA3; bar1Δ This study Fig. 2 G  
YFD230 w303 ste5Δ::ADE2 This study Parental 
yFD702 w303 ste5Δ::ADE2 Ste5S185D-3GFP::URA3 This study Fig. 3, A and B  
ySP85 w303 ste5Δ::ADE2 Ste5-3GFP::URA3 This study Fig. 3, A and B  
ySP86 w303 ste5Δ::ADE2 Ste5S185A-3GFP::URA3 This study Fig. 3, A and B  
ySP296 w303 ste5Δ::ADE2 Fig1qV::LEU2 This study Parental 
ySP309 w303 Fig1qV::LEU2 ADGEV::TRP1 This study Parental 
ySP323 w303 ste5Δ::ADE2 pRPS2-mCherry-TMD::HIS3 This study Fig. 4 B and Fig. 6, B and E–G  
ySP331 w303 Hta2-mCherry::URA3 Mpk1-GFP::HIS3 Lab collection Fig. S2 B 
ySR8 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5::HIS3 This study Fig. 3 C; Fig. 5, A and B; and Fig. S5, A and C 
ySR112 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5S185A::HIS3 This study Fig. 3, C and B; Fig. 5, A and B; and Fig. S5, A and C 
ySR116 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5S185D::HIS3 This study Fig. 3 C; and Fig. 5, A and B  
yBH56 BY4741 far1Δ::Far1-HA-2xStrp::CaURA3 This study Figs. 3 D and S3 
yBH80 BY4741 far1Δ::Far13A S208S210S211-HA-2xStrp::CaURA3 This study Fig. 3 D  
yBH90 BY4741 far1Δ::Far13E S208S210S211-HA-2xStrp::CaURA3 This study Fig. 3 D  
L40 AOP-HIS3::LYS2 LexAOP-LacZ::URA3 his3Δ leu2Δ trp1Δ ade2Δ Lab collection Fig. S5, A and B 
yIS153 W303a TMD-cherry-pRPS2::LEU2 far1Δ::Far13A S208S210S211::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS155 w303a TMD-cherry-pRPS2::LEU2 far1Δ::Far1::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS156 w303a TMD-cherry-pRPS2::LEU2 far1Δ::Far1::URA3 ste5Δ::Ste5::HIS3 This study Fig. 6, C and D  
yIS158 w303alpha far1Δ::Far1::URA3 ste5Δ::Ste5::HIS3 This study Fig. 6, C and D  
yIS159 w303alpha far1Δ::Far13A S208S210S211::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS163 w303alpha far1Δ::Far1::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
NameGenotypeSourceUsed in figure
BY4741 MATa his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 OpenBiosystems Parental 
w303 leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 Lab collection Parental 
yRM119 BY4741 Prps2-Ste7DS(1-30)-2xNLS(9SP)-Cherry::URA3 hta2Δ::Hta2-CFP::HIS3 Durandau et al., 2015  Fig. 1, D and E; and Fig. S1 A 
yRM120 BY4741 pkc1Δ::PKC1-GFP-HIS3MX6 ura3Δ::TMD-dCherry Mishra et al., 2017  Fig. S2 A 
yRM230 BY4741 Bni1-qV::URA3 TMD-dCherry::LEU2 fus3Δ::Fus3-as1::HIS3 Mishra et al., 2017  Fig. 1 F  
yRM242 w303 ste5Δ::ADE2, STE5-tV::LEU2 This study Fig. 1, A–C, G, and H; Fig. 5, C and D; and Fig. S2, C–F 
yRM243 w303 ste5Δ::ADE2, ste5Δ:: Ste5S185A-tV::LEU2 This study Fig. 5, C and D  
yRM244 yRM242 mpk1Δ::kanMX This study Fig. 1, G and H; and Fig. S2 C 
yRM251 yRM242 empty - TRP1 This study Fig. S2 G 
yRM252 yRM242 BCK1-20::TRP1 This study Fig. S2 G 
yRM253 BY4741 gat1Δ::GAT1-GFP-HIS3MX6 Collection Fig. S1 B 
yRM254 BY4741 sfp1Δ::SFP1-GFP-HIS3MX6 Collection Fig. S1 C 
WR232 w303 SILAC STE5-HTBn::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study, W303 SILAC background described in Reiter et al., 2012  Figs. 2 A and S3 
WR254 w303 SILAC FAR1-HTBeaq::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR484 w303 SILAC STE5-HTBn::hphMX, FAR1-HTBeaq::hphMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR658 w303 SILAC Ste5HTBnp::hphMX, ste7Δ::HIS3, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
WR725 w303 Ste5-HTBn::hphMX, ste7Δ::HIS3, ste12Δ::kanMX, sst2Δ::natMX This study Figs. 2 A and S3 
WR800 w303 SILAC STE5-HTB::hphMX, stl2Δ::kanMX, lys1Δ::kanMX, arg4Δ::kanMX, CAN1 This study Figs. 2 A and S3 
JA596 w303 STE5-HKMT-myc::LEU2; PKC1-protA-H3-HA::URA3; bar1Δ This study Fig. 2 G  
YFD230 w303 ste5Δ::ADE2 This study Parental 
yFD702 w303 ste5Δ::ADE2 Ste5S185D-3GFP::URA3 This study Fig. 3, A and B  
ySP85 w303 ste5Δ::ADE2 Ste5-3GFP::URA3 This study Fig. 3, A and B  
ySP86 w303 ste5Δ::ADE2 Ste5S185A-3GFP::URA3 This study Fig. 3, A and B  
ySP296 w303 ste5Δ::ADE2 Fig1qV::LEU2 This study Parental 
ySP309 w303 Fig1qV::LEU2 ADGEV::TRP1 This study Parental 
ySP323 w303 ste5Δ::ADE2 pRPS2-mCherry-TMD::HIS3 This study Fig. 4 B and Fig. 6, B and E–G  
ySP331 w303 Hta2-mCherry::URA3 Mpk1-GFP::HIS3 Lab collection Fig. S2 B 
ySR8 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5::HIS3 This study Fig. 3 C; Fig. 5, A and B; and Fig. S5, A and C 
ySR112 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5S185A::HIS3 This study Fig. 3, C and B; Fig. 5, A and B; and Fig. S5, A and C 
ySR116 w303 ste5Δ::ADE2 Fig1qV::LEU2 Ste5S185D::HIS3 This study Fig. 3 C; and Fig. 5, A and B  
yBH56 BY4741 far1Δ::Far1-HA-2xStrp::CaURA3 This study Figs. 3 D and S3 
yBH80 BY4741 far1Δ::Far13A S208S210S211-HA-2xStrp::CaURA3 This study Fig. 3 D  
yBH90 BY4741 far1Δ::Far13E S208S210S211-HA-2xStrp::CaURA3 This study Fig. 3 D  
L40 AOP-HIS3::LYS2 LexAOP-LacZ::URA3 his3Δ leu2Δ trp1Δ ade2Δ Lab collection Fig. S5, A and B 
yIS153 W303a TMD-cherry-pRPS2::LEU2 far1Δ::Far13A S208S210S211::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS155 w303a TMD-cherry-pRPS2::LEU2 far1Δ::Far1::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS156 w303a TMD-cherry-pRPS2::LEU2 far1Δ::Far1::URA3 ste5Δ::Ste5::HIS3 This study Fig. 6, C and D  
yIS158 w303alpha far1Δ::Far1::URA3 ste5Δ::Ste5::HIS3 This study Fig. 6, C and D  
yIS159 w303alpha far1Δ::Far13A S208S210S211::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
yIS163 w303alpha far1Δ::Far1::URA3 ste5Δ::Ste5S185A::HIS3 This study Fig. 6, C and D  
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