| Sample (n = 3) . | Cells with >10-μm protrusions (%) . | Cells with TNT-like protrusions (%) . | Cells with one TNT-like protrusion (%) . | Cells with more than one TNT-like protrusion (%) . |
|---|---|---|---|---|
| GFP | 24.23 ± 1.93 | 8.81 ± 2.92 | 8.36 ± 2.14 | 0.45 ± 0.78 |
| Rhes WT | 71.01 ± 6.49 | 36.29 ± 1.64 | 19.66 ± 1.72 | 16.93 ± 2.97 |
| Rhes S33N | 49.21 ± 1.16 | 10.25 ± 1.97 | 8.91 ± 1.76 | 1.33 ± 0.23 |
| Rhes C263S | 34.94 ± 5.48 | 10.78 ± 2.93 | 8.85 ± 2.68 | 1.92 ± 0.82 |
| Rhes 1–171 | 48.64 ± 5.35 | 13.87 ± 0.75 | 10.65 ± 0.28 | 3.22 ± 0.60 |
| Rhes 171–266 | 50.63 ± 7 0.69 | 27.29 ± 1.75 | 20.88 ± 2.07 | 6.30 ± 0.72 |
| Sample (n = 3) . | Cells with >10-μm protrusions (%) . | Cells with TNT-like protrusions (%) . | Cells with one TNT-like protrusion (%) . | Cells with more than one TNT-like protrusion (%) . |
|---|---|---|---|---|
| GFP | 24.23 ± 1.93 | 8.81 ± 2.92 | 8.36 ± 2.14 | 0.45 ± 0.78 |
| Rhes WT | 71.01 ± 6.49 | 36.29 ± 1.64 | 19.66 ± 1.72 | 16.93 ± 2.97 |
| Rhes S33N | 49.21 ± 1.16 | 10.25 ± 1.97 | 8.91 ± 1.76 | 1.33 ± 0.23 |
| Rhes C263S | 34.94 ± 5.48 | 10.78 ± 2.93 | 8.85 ± 2.68 | 1.92 ± 0.82 |
| Rhes 1–171 | 48.64 ± 5.35 | 13.87 ± 0.75 | 10.65 ± 0.28 | 3.22 ± 0.60 |
| Rhes 171–266 | 50.63 ± 7 0.69 | 27.29 ± 1.75 | 20.88 ± 2.07 | 6.30 ± 0.72 |