| siRNA . | Defects observed in 3D cyst culture . | Defects observed in 2D cell culture . | Defects in polarized 2D cells . | ||
|---|---|---|---|---|---|
| Rab3A | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab3B | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab3D | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab4A/B | +/− | Some multiple lumens | − | − | |
| Rab5A | + | Some multiple lumens; reduced proliferation | + | Lateral lumens | + |
| Rab5B | + | PCX retention in large actin-rich vacuoles | + | Lateral lumens | + |
| Rab5C | − | − | − | ||
| Rab8A | + | Intracellular retention of PCX near the apical site | + | Intracellular retention of PCX | + |
| Rab10 | − | − | − | ||
| Rab11A | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Delayed vesicle docking | − |
| Rab12 | + | Retention of PCX in multiple large dots | + | Retention of PCX in multiple actin-free dots | − |
| Rab13 | − | + | Intracellular retention of PCX | + | |
| Rab14 | − | + | Delayed vesicle internalization | − | |
| Rab15 | + | Intracellular retention of PCX | − | Increased spreading | − |
| Rab17 | + | PCX retention in small intracellular vesicles | + | PCX retention near cell–cell contact sites | + |
| Rab19 | + | Intracellular retention of PCX | − | Increased spreading | − |
| Rab20 | − | − | − | ||
| Rab23 | − | − | − | ||
| Rab24 | − | Reduced proliferation | − | − | |
| Rab25 | + | Intracellular retention of PCX near the apical site | − | − | |
| Rab27A | + | Intracellular retention of PCX near the apical site | +/− | Slight intracellular retention of PCX | +/− |
| Rab32 | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Lateral lumens | + |
| Rab34 | − | − | − | ||
| Rab35 | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Intracellular retention of PCX in actin-rich clusters | + |
| siRNA . | Defects observed in 3D cyst culture . | Defects observed in 2D cell culture . | Defects in polarized 2D cells . | ||
|---|---|---|---|---|---|
| Rab3A | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab3B | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab3D | + | Intracellular retention of PCX near the apical site | +/− | PCX retention near cell–cell contact sites | − |
| Rab4A/B | +/− | Some multiple lumens | − | − | |
| Rab5A | + | Some multiple lumens; reduced proliferation | + | Lateral lumens | + |
| Rab5B | + | PCX retention in large actin-rich vacuoles | + | Lateral lumens | + |
| Rab5C | − | − | − | ||
| Rab8A | + | Intracellular retention of PCX near the apical site | + | Intracellular retention of PCX | + |
| Rab10 | − | − | − | ||
| Rab11A | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Delayed vesicle docking | − |
| Rab12 | + | Retention of PCX in multiple large dots | + | Retention of PCX in multiple actin-free dots | − |
| Rab13 | − | + | Intracellular retention of PCX | + | |
| Rab14 | − | + | Delayed vesicle internalization | − | |
| Rab15 | + | Intracellular retention of PCX | − | Increased spreading | − |
| Rab17 | + | PCX retention in small intracellular vesicles | + | PCX retention near cell–cell contact sites | + |
| Rab19 | + | Intracellular retention of PCX | − | Increased spreading | − |
| Rab20 | − | − | − | ||
| Rab23 | − | − | − | ||
| Rab24 | − | Reduced proliferation | − | − | |
| Rab25 | + | Intracellular retention of PCX near the apical site | − | − | |
| Rab27A | + | Intracellular retention of PCX near the apical site | +/− | Slight intracellular retention of PCX | +/− |
| Rab32 | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Lateral lumens | + |
| Rab34 | − | − | − | ||
| Rab35 | + | Intracellular retention of PCX near the apical site/scattered vesicles | + | Intracellular retention of PCX in actin-rich clusters | + |
Defects in PCX localization are shown by + (clear defect), +/− (mild defect), and − (no defect). Detailed image data are shown in Figs. 4, 5, S4, and S5.